Repression of the lysogenic PR promoter in bacteriophage TP901-1 through binding of a CI-MOR complex to a composite OM-OR operator

Autor: K.K. Rasmussen, Kim Sneppen, Karin Hammer, Mogens Kilstrup, Jesper Tvenge Neergaard, Margit Pedersen, Leila Lo Leggio, Johan Cassias

Publikováno v: Scientific Reports, Vol 10, Iss 1, Pp 1-13 (2020)
Pedersen, M, Neergaard, J T, Cassias, J, Rasmussen, K K, Lo Leggio, L, Sneppen, K, Hammer, K & Kilstrup, M 2020, ' Repression of the lysogenic P R promoter in bacteriophage TP901-1 through binding of a CI-MOR complex to a composite O M-O R operator ', Scientific Reports, vol. 10, no. 1, 8659 . https://doi.org/10.1038/s41598-020-65493-0
Scientific Reports

A functional genetic switch from the lactococcal bacteriophage TP901-1, deciding which of two divergently transcribing promoters becomes most active and allows this bi-stable decision to be inherited in future generations requires a DNA region of les

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e968d35c2920a81be0a2037b2a0c291f
http://link.springer.com/article/10.1038/s41598-020-65493-0

Predictable tuning of protein expression in bacteria

Autor: Scott James Harrison, Margit Pedersen, Alex Toftgaard Nielsen, Mads Bonde, Markus J. Herrgård, Sheila Ingemann Jensen, Morten Otto Alexander Sommer, Tune Wulff, Michael Schantz Klausen

Publikováno v: Nature Methods. 13:233-236

We comprehensively assessed the contribution of the Shine-Dalgarno sequence to protein expression and used the data to develop EMOPEC (Empirical Model and Oligos for Protein Expression Changes; http://emopec.biosustain.dtu.dk). EMOPEC is a free tool

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::442c68fdb2c97d28e8879ff41788ed05
https://doi.org/10.1038/nmeth.3727

Structural and dynamics studies of a truncated variant of CI repressor from bacteriophage TP901-1

Autor: Karin Hammer, Malene Ringkjøbing Jensen, Elisabetta Boeri Erba, K.K. Rasmussen, Martin Blackledge, Mogens Kilstrup, Margit Pedersen, Anders K. Varming, Leila Lo Leggio, Peter W. Thulstrup, Kristian E. H. Frandsen

Publikováno v: Rasmussen, K K, Frandsen, K E H, Erba, E B, Pedersen, M, Varming, A K, Hammer, K, Kilstrup, M, Thulstrup, P W, Blackledge, M, Jensen, M R & Lo Leggio, L 2016, ' Structural and dynamics studies of a truncated variant of CI repressor from bacteriophage TP901-1 ', Scientific Reports, vol. 6, 29574 . https://doi.org/10.1038/srep29574
'Scientific Reports ', vol: 6, pages: 29574-1-29574-12 (2016)
Scientific Reports
Scientific Reports, 2016, 6, pp.29574. ⟨10.1038/srep29574⟩
Scientific Reports, Nature Publishing Group, 2016, 6, pp.29574. ⟨10.1038/srep29574⟩
Scientific reports 6, 29574 (2016). doi:10.1038/srep29574
Rasmussen, K K, Frandsen, K E H, Boeri Erba, E, Pedersen, M, Varming, A K, Hammer, K, Kilstrup, M, Thulstrup, P W, Blackledge, M, Jensen, M R & Lo Leggio, L 2016, ' Structural and dynamics studies of a truncated variant of CI repressor from bacteriophage TP901-1 ', Scientific Reports, vol. 6, 29574 . https://doi.org/10.1038/srep29574

The CI repressor from the temperate bacteriophage TP901-1 consists of two folded domains, an N-terminal helix-turn-helix DNA-binding domain (NTD) and a C-terminal oligomerization domain (CTD), which we here suggest to be further divided into CTD1 and

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::01248eec78272bc1e77779c3863f7bca

Modeling of the Genetic Switch of Bacteriophage TP901-1: A Heteromer of CI and MOR Ensures Robust Bistability

Autor: Margit Pedersen, Anne K. Alsing, Kim Sneppen, Hiizu Nakanishi

Publikováno v: Journal of Molecular Biology. 394:15-28

The lytic-lysogenic switch of the temperate lactococcal phage TP901-1 is fundamentally different from that of phage lambda. In phage TP901-1, the lytic promoter PL is repressed by CI whereas repression of the lysogenic promoter PR requires the presen

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::77ad4247a6abf5d1a3734ec921187a5d
https://doi.org/10.1016/j.jmb.2009.08.075

Mutational analysis of the activator of late transcription, Alt, in the lactococcal bacteriophage TP901-1

Autor: Karin Hammer, Margit Pedersen

Publikováno v: Archives of Virology. 152:305-320

An activator protein, Alt, synthesized during the early state of lytic infection is required for transcription of the late operon in the lactococcal phage TP901-1. In order to identify amino acid residues in the Alt protein required for activation of

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a0cbd95309c5d19f07f90162b7009e10
https://doi.org/10.1007/s00705-006-0851-7