Výsledky vyhledávání - "Margaret T. Butko"

Second-harmonic generation-based methods to detect and characterize ligand-induced RNA conformational changes

Autor: Yuliya Birman, Sina Khorsand, Erick Tu, Margaret T. Butko, Richard B. Mortensen

Publikováno v: Methods. 167:92-104

Second-harmonic generation (SHG) is a biophysical tool that senses ligand-induced conformational changes in biomolecules. The Biodesy Delta™ has been developed as a high-throughput screening platform to monitor conformational changes in proteins an

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f224ff892a0781608c478f4068db59d6
https://doi.org/10.1016/j.ymeth.2019.05.012

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Discovery of fragments inducing conformational effects in dynamic proteins using a second-harmonic generation biosensor

Autor: Edward A, FitzGerald, Margaret T, Butko, Pierre, Boronat, Daniela, Cederfelt, Mia, Abramsson, Hildur, Ludviksdottir, Jacqueline E, van Muijlwijk-Koezen, Iwan J P, de Esch, Doreen, Dobritzsch, Tracy, Young, U Helena, Danielson

Publikováno v: RSC advances. 11(13)

Biophysical screening of compound libraries for the identification of ligands that interact with a protein is efficient, but does typically not reveal if (or how) ligands may interfere with its functional properties. For this a biochemical/functional

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::3ffad0a15c8299db2e7bd6cbc46c99bd
https://pubmed.ncbi.nlm.nih.gov/35423271

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Multicolor Electron Microscopy for Simultaneous Visualization of Multiple Molecular Species

Autor: Eric A. Bushong, Roger Y. Tsien, Sakina F Palida Lemieux, Stephen R. Adams, Mark H. Ellisman, Paul Steinbach, Mason R. Mackey, Ranjan Ramachandra, Margaret T. Butko, Ben N G Giepmans

Publikováno v: Cell chemical biology, vol 23, iss 11
Cell Chemical Biology, 23(11), 1417-1427. CELL PRESS

Electron microscopy (EM) remains the primary method for imaging cellular and tissue ultrastructure, although simultaneous localization of multiple specific molecules continues to be a challenge for EM. We present a method for obtaining multicolor EM

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::902dc68568afd8faad46a672ef934c63
https://doi.org/10.1016/j.chembiol.2016.10.006

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Discovery of Selective RNA-Binding Small Molecules by Affinity-Selection Mass Spectrometry

Autor: John A. Howe, Julja Burchard, Gabriel Mercado, Graham Smith, Daniel J. Klein, Margaret T. Butko, Scott S. Walker, Thierry O. Fischmann, Corey Strickland, Hai-Young Kim, Elliott B. Nickbarg, Alan Hruza, Ali Nahvi, Noreen F. Rizvi, Matthew Richards, Mark A. McCoy, Peter J. Dandliker, Chad Chamberlin, Peter Saradjian

Publikováno v: ACS chemical biology. 13(3)

Recent advances in understanding the relevance of noncoding RNA (ncRNA) to disease have increased interest in drugging ncRNA with small molecules. The recent discovery of ribocil, a structurally distinct synthetic mimic of the natural ligand of the f

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2b49ae92950caad1b013beb70995111b
https://pubmed.ncbi.nlm.nih.gov/29412640

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Second-Harmonic Generation (SHG) for Conformational Measurements: Assay Development, Optimization, and Screening

Autor: Juan J. Carrillo, Ben Moree, John Strelow, Mary Geck Do, Joshua Salafsky, Tracy A. Young, Bason Clancy, Tarun Gheyi, Margaret T. Butko

Second-harmonic generation (SHG) has recently emerged as a biophysical tool for conformational sensing of a target biomolecule upon binding to ligands such as small molecules, fragments, proteins, peptides, and oligonucleotides. To date, SHG has been

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::bd85075312fdb7e806432d39d62ac79a
https://doi.org/10.1016/bs.mie.2018.09.017

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Recombinant Antibody Color Resulting from Advanced Glycation End Product Modifications

Autor: Hilary Pallat, X. Christopher Yu, Armando J. Cordoba, Margaret T. Butko

Publikováno v: Analytical Chemistry. 86:9816-9823

Recombinant antibodies produced in Chinese hamster ovary (CHO) cells often exhibit a slight yellow-brown color, but the molecular basis for this color has remained elusive. We predicted that the color results from post-translational modifications on

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6efa6a42b058cf92aef3f0013e06e7ee
https://doi.org/10.1021/ac5024099

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Detection of Ligand-Induced Conformational Changes in Oligonucleotides by Second-Harmonic Generation at a Supported Lipid Bilayer Interface

Autor: Margaret T. Butko, Ben Moree, Richard B. Mortensen, Joshua Salafsky

Publikováno v: Analytical chemistry. 88(21)

There is a high demand for characterizing oligonucleotide structural changes associated with binding interactions as well as identifying novel binders that modulate their structure and function. In this study, second-harmonic generation (SHG) was use

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7f112677942f2aac68207a0c61271362
https://pubmed.ncbi.nlm.nih.gov/27696827

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ATP-Competitive MLKL Binders Have No Functional Impact on Necroptosis

Autor: Joshua Salafsky, Ti Wang, Xin E. Sun, Bin Ma, Klaus Michelsen, Laura Silvian, Timothy D. McKee, Murugan Paramasivam, John Howard Jones, Margaret T. Butko, Andrea Bertolotti-Ciarlet, Douglas Marcotte, Ben Moree

Publikováno v: PLoS ONE
PLoS ONE, Vol 11, Iss 11, p e0165983 (2016)

MLKL is a pore forming pseudokinase involved in the final stage of necroptosis, a form of programmed cell death. Its phosphorylation by RIPK3 is necessary for triggering necroptosis but not for triggering apoptosis, which makes it a unique target for

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::37384f1d47e19dfb65e28d90cd0edd90
http://europepmc.org/articles/PMC5104457

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Fluorescent and photo-oxidizing TimeSTAMP tags track protein fates in light and electron microscopy

Autor: Jin Yang, Mason R. Mackey, Michael Z. Lin, Hyung J. Kim, Xiaokun Shu, Yang Geng, Margaret T. Butko, Roger Y. Tsien, Noo Li Jeon, Mark H. Ellisman

Publikováno v: Nature Neuroscience. 15:1742-1751

Protein synthesis is highly regulated throughout nervous system development, plasticity and regeneration. However, tracking the distributions of specific new protein species has not been possible in living neurons or at the ultrastructural level. Pre

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::144e2bea0517002530e8ff2bcea70329
https://doi.org/10.1038/nn.3246

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PKMζ, but not PKCλ, is rapidly synthesized and degraded at the neuronal synapse

Autor: Mason R. Mackey, Larry A. Gross, Sakina F. Palida, Roger Y. Tsien, John T. Ngo, Margaret T. Butko, Mark H. Ellisman

Publikováno v: The Journal of neuroscience : the official journal of the Society for Neuroscience, vol 35, iss 20

Synthesizing, localizing, and stabilizing new protein copies at synapses are crucial factors in maintaining the synaptic changes required for storing long-term memories. PKMζ recently emerged as a molecule putatively responsible for maintaining enco

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::277451b6886fc3ff549233bb356815a1
https://pubmed.ncbi.nlm.nih.gov/25995463

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