Zobrazeno 1 - 10
of 35
pro vyhledávání: '"Margaret Nutley"'
The negative transcriptional regulator NmrA discriminates between oxidized and reduced dinucleotides
Autor:
Kris Leslie, Paul Thompson, Alan Cooper, Margaret Nutley, Alastair R. Hawkins, A. Dodds, David K. Stammers, Christopher L. Johnson, Heather K. Lamb
NmrA, a transcription repressor involved in the regulation of nitrogen metabolism in Aspergillus nidulans,is a member of the short-chain dehydrogenase reductase superfamily. Isothermal titration calorimetry and differential scanning calorimetry have
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8799892ffbf4622153a0783addc1a50c
https://ora.ox.ac.uk/objects/uuid:883dcf17-13a6-41a3-91d3-833df6971a11
https://ora.ox.ac.uk/objects/uuid:883dcf17-13a6-41a3-91d3-833df6971a11
Autor:
Ana Cristina Sarmento, T. Cardoso, Anabela Pereira, Euclides Pires, Marlene Barros, Nicholas C. Price, Alan Cooper, Sharon M. Kelly, Cláudia S. Oliveira, Margaret Nutley, Thomas J. Jess
Publikováno v:
International Journal of Biological Macromolecules. 45:213-220
In order to increase understanding of the basis of the stability of the native conformational state of porcine pepsin A, a strategy based on induction and monitoring of protein denaturation was developed. Structural perturbation was achieved by addin
Autor:
Gareth A. Roberts, C. Logan Mackay, Margaret Nutley, Augoustinos S. Stephanou, Andrew R. Thomson, David T. F. Dryden, Alan Cooper, Laurie P. Cooper, David Clarke
Publikováno v:
Journal of Molecular Biology
Stephanou, A S, Roberts, G A, Cooper, L P, Clarke, D J, Thomson, A R, MacKay, C L, Nutley, M, Cooper, A & Dryden, D T F 2009, ' Dissection of the DNA Mimicry of the Bacteriophage T7 Ocr Protein using Chemical Modification ', Journal of Molecular Biology, vol. 391, no. 3, pp. 565-576 . https://doi.org/10.1016/j.jmb.2009.06.020
Stephanou, A S, Roberts, G A, Cooper, L P, Clarke, D J, Thomson, A R, MacKay, C L, Nutley, M, Cooper, A & Dryden, D T F 2009, ' Dissection of the DNA Mimicry of the Bacteriophage T7 Ocr Protein using Chemical Modification ', Journal of Molecular Biology, vol. 391, no. 3, pp. 565-576 . https://doi.org/10.1016/j.jmb.2009.06.020
The homodimeric Ocr (overcome classical restriction) protein of bacteriophage T7 is a molecular mimic of double-stranded DNA and a highly effective competitive inhibitor of the bacterial type I restriction/modification system. The surface of Ocr is r
Autor:
Margaret Nutley, Malcolm D. Walkinshaw, Cécile Tron, Alan Cooper, David Clarke, Iain W. McNae, Dominic J. Campopiano, Robert Baxter
Publikováno v:
Tron, C M, McNae, I W, Nutley, M, Clarke, D J, Cooper, A, Walkinshaw, M D, Baxter, R L & Campopiano, D J 2009, ' Structural and functional studies of the biotin protein ligase from Aquifex aeolicus reveal a critical role for a conserved residue in target specificity ', Journal of Molecular Biology, vol. 387, no. 1, pp. 129-46 . https://doi.org/10.1016/j.jmb.2008.12.086
Biotin protein ligase (BPL; EC 6.3.4.15) catalyses the formation of biotinyl-5'-AMP from biotin and ATP, and the succeeding biotinylation of the biotin carboxyl carrier protein. We describe the crystal structures, at 2.4 A resolution, of the class I
Autor:
Alan Cooper, Rachel Turkington, David T. F. Dryden, Margaret Nutley, Gareth A. Roberts, Emily H. Pritchard, Augoustinos S. Stephanou, Mark R. Tock
Publikováno v:
Biochemical and Biophysical Research Communications. 378:129-132
The ocr protein of bacteriophage T7 is a structural and electrostatic mimic of approximately 24 base pairs of double-stranded B-form DNA. As such, it inhibits all Type I restriction and modification (R/M) enzymes by blocking their DNA binding grooves
Autor:
Laurie P. Cooper, Margaret Nutley, Alan Cooper, Garry W. Blakely, John H. White, Gareth A. Roberts, Dimitra Serfiotis-Mitsa, David T. F. Dryden
Publikováno v:
Journal of Molecular Biology. 383:970-981
Gene orf18, which is situated within the intercellular transposition region of the conjugative transposon Tn916 from the bacterial pathogen Enterococcus faecalis, encodes a putative ArdA (alleviation of restriction of DNA A) protein. Conjugative tran
Autor:
Patrice Woisel, Gouher Rabani, Marc Bria, Alan Cooper, Graeme Cooke, Shanika Gunatilaka Hewage, François Delattre, Margaret Nutley
Publikováno v:
Tetrahedron Letters. 48:8430-8433
This Letter, describes the complexation of α-, β- and γ-cyclodextrins (1–3) with TTF derivative 4 in water. In particular, we show using 1H, 13C NMR, UV–vis spectroscopy and isothermal titration calorimetry that β-cyclodextrin 2 forms an effe
Autor:
James F. Garety, Marc Bria, Shanika Gunatilaka Hewage, Margaret Nutley, Graeme Cooke, Patrice Woisel, Alan Cooper, Gouher Rabani
Publikováno v:
Tetrahedron Letters. 48:301-304
This article reports the characterization of pseudorotaxanes fabricated from cyclobis(paraquat-p-phenylene) 1, and 1,4-dialkyloxyphenyl derivative 2 or 1,5-dialkyloxynaphthalene derivative 3 in water. Addition of competing guest 3 to 1.2 or the elect
Autor:
Patrick T. Gunning, Arkady Ellern, Margaret Nutley, Paul Kögerler, John Fielden, De-Liang Long, Leroy Cronin
Publikováno v:
Polyhedron. 25:3474-3480
The coordination chemistry of the tetradentate pyridyl N-donor ligand cis-3,5-bis-[2-pyridinyleneamin]-trans-hydroxycyclohexane (DDOP) has been investigated with zinc(II) nitrate and triflate. The resulting complexes, [Zn(DDOP)(H2O)(NO3)](NO3) (1), a
Autor:
Neil Errington, Stephen E. Harding, Alan Cooper, Margaret Nutley, Celia F. Goodhew, Françoise Guerlesquin, Graham W. Pettigrew, José J. G. Moura, Sofia R. Pauleta, Isabel Moura
Publikováno v:
Biochemistry. 43:14566-14576
Pseudoazurin binds at a single site on cytochrome c peroxidase from Paracoccus pantotrophus with a K(d) of 16.4 microM at 25 degrees C, pH 6.0, in an endothermic reaction that is driven by a large entropy change. Sedimentation velocity experiments co