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Autor:
François Daigle, Ngiep Man Hua, Dimitri Vzdornov, George Szatmari, Margaret D. Mamet-Bratley, Barbara Karska-Wysocki, Wanda Smoragiewicz
Publikováno v:
Journal of Biotechnology. 121:144-153
A new restriction endonuclease LlaMI has been characterized in Lactococcus lactis subsp. cremoris M19. LlaMI recognizes the sequence 5'-CCNGG-3' and cuts after the second cytosine. This restriction endonuclease is related to commercially available Sc
Publikováno v:
Journal of Photochemistry and Photobiology B: Biology. 38:48-53
Psoralens react photochemically with DNA to form interstrand crosslinks as well as two types of monoadduct (furan-side and pyrone-side adducts). To investigate the relative roles of these adducts in toxicity, we have studied the interaction of 4,5′
Publikováno v:
Mutation Research Letters. 325:39-45
We have examined protein synthesis directed by bacteriophage T7 which had been alkylated with methyl methanesulfonate so as to produce apurinic sites in its DNA in vivo. Both repair-proficient and repair-deficient (xth nfo mutant) strains of Escheric
Publikováno v:
Environmental and Molecular Mutagenesis. 23:32-36
The effects of abasic (AP) sites on RNA synthesis were studied in vitro, using T7 RNA polymerase and a plasmid template containing a T7 promoter. The presence of increasing numbers of AP sites caused a progressive decline in RNA synthesis. The averag
Publikováno v:
Mutation Research/DNA Repair. 294:285-298
Treatment of bacteriophage T7 with methyl methanesulfonate perturbed phage-specific genetic expression in both repair-proficient and repair-deficient Escherichia coli cells. In wild-type cells (AB1157), the time course of protein synthesis was slowed
Publikováno v:
Biochemistry and Cell Biology. 70:605-608
The development of bacteriophage T7 was examined in an Escherichia coli double mutant defective for the two major apurinic, apyrimidinic endonucleases (exonuclease III and endonuclease IV, xth nfo). In cells infected with phages containing apurinic s
Autor:
Guy Czaika, Margaret D. Mamet-Bratley
Publikováno v:
Biochimica et biophysica acta. 1130(1)
DNA injection by alkylated and nonalkylated bacteriophage T7 has been analyzed by a physical method which involved Southern hybridization to identify noninjected regions of DNA. Treatment of phage with methyl methanesulfonate reduced the amount of DN
Autor:
Margaret D. Mamet-Bratley
Publikováno v:
Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis. 340:237-243
T7 DNA containing a limited number of apurinic sites shows reduced template activity for RNA synthesis in vitro. Initiation specificity remains unchanged by the presence of apurinic sites. Quantitative analysis shows that depurination is much more de
Publikováno v:
Mutation Research/DNA Repair Reports. 166:1-8
Quantitative analysis of DNA replication, in E. coli B cells infected by methyl methanesulfonate-treated bacteriophage T7, showed that production of phage DNA was delayed and decreased. The cause of the delay appeared to be a delay in host-DNA breakd