Zobrazeno 1 - 10
of 20
pro vyhledávání: '"Maren Schiwon"'
Publikováno v:
Molecular Therapy: Nucleic Acids, Vol 14, Iss , Pp 364-376 (2019)
Gene therapy represents an attractive alternative to treat hemophilia B. Here we established three hepatocyte-derived cell lines based on Huh7, PLC/PRF/5, and Hep3B cells stably carrying a mutated canine FIX (cFIXmut) transgene containing a single po
Externí odkaz:
https://doaj.org/article/287c72adbb7147ff8d4489c7608fcf46
Autor:
Raphael L. Tsoukas, Wolfram Volkwein, Jian Gao, Maren Schiwon, Nora Bahlmann, Thomas Dittmar, Claudia Hagedorn, Eric Ehrke-Schulz, Wenli Zhang, Armin Baiker, Anja Ehrhardt
Publikováno v:
Cells, Vol 11, Iss 5, p 841 (2022)
To develop adenoviral cell- or tissue-specific gene delivery, understanding of the infection mechanisms of adenoviruses is crucial. Several adenoviral attachment proteins such as CD46, CAR and sialic acid have been identified and studied. However, mo
Externí odkaz:
https://doaj.org/article/077013b8e3424060bf933ba3d702382d
Autor:
Maren Schiwon, Eric Ehrke-Schulz, Andreas Oswald, Thorsten Bergmann, Thomas Michler, Ulrike Protzer, Anja Ehrhardt
Publikováno v:
Molecular Therapy: Nucleic Acids, Vol 12, Iss , Pp 242-253 (2018)
High-capacity adenoviral vectors (HCAdVs) devoid of all coding genes are powerful tools to deliver large DNA cargos into cells. Here HCAdVs were designed to deliver a multiplexed complete CRISPR/Cas9 nuclease system or a complete pair of transcriptio
Externí odkaz:
https://doaj.org/article/3c370be32eae4fd284bfd916635c51f3
Autor:
Eric Ehrke-Schulz, Maren Schiwon, Theo Leitner, Stephan Dávid, Thorsten Bergmann, Jing Liu, Anja Ehrhardt
Publikováno v:
Scientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
Abstract The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9 system revolutionized the field of gene editing but viral delivery of the CRISPR/Cas9 system has not been fully explored. Here we adapted clinically relevant high-ca
Externí odkaz:
https://doaj.org/article/af66499d641d4b4c9339d899743e75fc
Autor:
Wenli Zhang, Jun Fu, Jing Liu, Hailong Wang, Maren Schiwon, Sebastian Janz, Lukas Schaffarczyk, Lukas von der Goltz, Eric Ehrke-Schulz, Johannes Dörner, Manish Solanki, Philip Boehme, Thorsten Bergmann, Andre Lieber, Chris Lauber, Andreas Dahl, Andreas Petzold, Youming Zhang, A. Francis Stewart, Anja Ehrhardt
Publikováno v:
Cell Reports, Vol 19, Iss 8, Pp 1698-1709 (2017)
Adenoviruses (Ads) are large human-pathogenic double-stranded DNA (dsDNA) viruses presenting an enormous natural diversity associated with a broad variety of diseases. However, only a small fraction of adenoviruses has been explored in basic virology
Externí odkaz:
https://doaj.org/article/05491b28e9a94eaaac46bab0120beb0a
Publikováno v:
Cancers, Vol 12, Iss 7, p 1934 (2020)
Human papillomaviruses (HPV) cause malignant epithelial cancers including cervical carcinoma, non-melanoma skin and head and neck cancer. They drive tumor development through the expression of their oncoproteins E6 and E7. Designer nucleases were sho
Externí odkaz:
https://doaj.org/article/47168b9ce95c49a789ccf911550773f2
Quantification of designer nuclease induced mutation rates: a direct comparison of different methods
Autor:
Eric Ehrke-Schulz, Thorsten Bergmann, Maren Schiwon, Johannes Doerner, Kamola Saydaminova, Andre Lieber, Anja Ehrhardt
Publikováno v:
Molecular Therapy: Methods & Clinical Development, Vol 3, Iss C (2016)
Designer nucleases are broadly applied to induce site-specific DNA double-strand breaks (DSB) in genomic DNA. These are repaired by nonhomologous end joining leading to insertions or deletions (in/dels) at the respective DNA-locus. To detect in/del m
Externí odkaz:
https://doaj.org/article/35b92eeca55440ae8760d06dfaab68d3
Publikováno v:
Molecular Therapy: Nucleic Acids, Vol 14, Iss, Pp 364-376 (2019)
Molecular Therapy. Nucleic Acids
Molecular Therapy. Nucleic Acids
Gene therapy represents an attractive alternative to treat hemophilia B. Here we established three hepatocyte-derived cell lines based on Huh7, PLC/PRF/5, and Hep3B cells stably carrying a mutated canine FIX (cFIXmut) transgene containing a single po
Publikováno v:
Cancers, Vol 12, Iss 1934, p 1934 (2020)
Cancers
Volume 12
Issue 7
Cancers
Volume 12
Issue 7
Human papillomaviruses (HPV) cause malignant epithelial cancers including cervical carcinoma, non-melanoma skin and head and neck cancer. They drive tumor development through the expression of their oncoproteins E6 and E7. Designer nucleases were sho
Autor:
Thorsten Bergmann, Verena Schildgen, Eric Ehrke-Schulz, Jian Gao, Oliver Schildgen, Anja Ehrhardt, Maren Schiwon, Stephan David
Publikováno v:
The journal of gene medicine. 20(5)
BACKGROUND Gene correction at specific target loci provides a powerful strategy for overcoming genetic diseases. In the present study, we aimed to use an in vitro model for canine hemophilia B containing a single point mutation in the catalytic domai