Zobrazeno 1 - 10
of 24
pro vyhledávání: '"Marek Sebesta"'
Autor:
Katerina Linhartova, Francesco Luca Falginella, Martin Matl, Marek Sebesta, Robert Vácha, Richard Stefl
Publikováno v:
Nature Communications, Vol 15, Iss 1, Pp 1-17 (2024)
Abstract The intrinsically disordered carboxy-terminal domain (CTD) of the largest subunit of RNA Polymerase II (RNAPII) consists of multiple tandem repeats of the consensus heptapeptide Y1-S2-P3-T4-S5-P6-S7. The CTD promotes liquid-liquid phase-sepa
Externí odkaz:
https://doaj.org/article/c89a2875d34c461db455c1f2a4eeae34
Autor:
Qilin Long, Marek Sebesta, Katerina Sedova, Vojtech Haluza, Adele Alagia, Zhichao Liu, Richard Stefl, Monika Gullerova
Publikováno v:
Cell Reports, Vol 42, Iss 12, Pp 113489- (2023)
Summary: Double-strand breaks (DSBs) are the most severe type of DNA damage. Previously, we demonstrated that RNA polymerase II (RNAPII) phosphorylated at the tyrosine 1 (Y1P) residue of its C-terminal domain (CTD) generates RNAs at DSBs. However, th
Externí odkaz:
https://doaj.org/article/7c47db390a97410190d2f9062b9b5371
Autor:
Christopher J. Carnie, Lucy Armstrong, Marek Sebesta, Antonio Ariza, Xiaomeng Wang, Emily Graham, Kang Zhu, Dragana Ahel
Publikováno v:
Cell Reports, Vol 42, Iss 4, Pp 112329- (2023)
Summary: Structurally complex genomic regions, such as centromeres, are inherently difficult to duplicate. The mechanism behind centromere inheritance is not well understood, and one of the key questions relates to the reassembly of centromeric chrom
Externí odkaz:
https://doaj.org/article/ce3976c83bf2446ba07dcf54342001a7
Autor:
Lisa-Marie Appel, Vedran Franke, Melania Bruno, Irina Grishkovskaya, Aiste Kasiliauskaite, Tanja Kaufmann, Ursula E. Schoeberl, Martin G. Puchinger, Sebastian Kostrhon, Carmen Ebenwaldner, Marek Sebesta, Etienne Beltzung, Karl Mechtler, Gen Lin, Anna Vlasova, Martin Leeb, Rushad Pavri, Alexander Stark, Altuna Akalin, Richard Stefl, Carrie Bernecky, Kristina Djinovic-Carugo, Dea Slade
Publikováno v:
Nature Communications, Vol 12, Iss 1, Pp 1-24 (2021)
Here the authors identify PHF3 SPOC domain as a reader of the phosphorylated RNA polymerase II (Pol II) C-terminal domain. They show that PHF3 clusters with Pol II complexes in cells, drives phase separation of Pol II in vitro, and regulates neuronal
Externí odkaz:
https://doaj.org/article/248ef7e0ffd543019b8945fa5bb593d6
Publikováno v:
BMC Biology, Vol 15, Iss 1, Pp 1-17 (2017)
Abstract Background Proper DNA replication is essential for faithful transmission of the genome. However, replication stress has serious impact on the integrity of the cell, leading to stalling or collapse of replication forks, and has been determine
Externí odkaz:
https://doaj.org/article/121410ae72b546699f2b61ab772eec5f
Publikováno v:
Nature Communications, Vol 8, Iss 1, Pp 1-16 (2017)
ZRANB3 (Zinc-finger, RAN-Binding domain containing 3) is a structure-specific endonuclease that is recruited to DNA breaks and stressed replication forks. Here the authors present the crystal structure of the ZRANB3 endonuclease domain and analyse ho
Externí odkaz:
https://doaj.org/article/e255a9bc2f6248c5a18735606cd0c8fe
Autor:
Rebecca C Burgess, Marek Sebesta, Alexandra Sisakova, Victoria P Marini, Michael Lisby, Jiri Damborsky, Hannah Klein, Rodney Rothstein, Lumir Krejci
Publikováno v:
PLoS ONE, Vol 8, Iss 12, p e82630 (2013)
Rad54 is an ATP-driven translocase involved in the genome maintenance pathway of homologous recombination (HR). Although its activity has been implicated in several steps of HR, its exact role(s) at each step are still not fully understood. We have i
Externí odkaz:
https://doaj.org/article/64188a082a04436cb02cf3915790e109
Autor:
Qilin Long, Marek Sebesta, Katerina Sedova, Vojtech Haluza, Adele Alagia, Zhichao Liu, Richard Stefl, Monika Gullerova
The most toxic forms of DNA damage are double-strand breaks (DSBs). We have previously shown that RNA polymerase II (RNAPII), phosphorylated at tyrosine 1 (Y1P) on the C- terminal domain, transcribes RNA at DSBs to promote efficient DNA repair. Howev
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::064618b4bbfaddc54f4e0f81f2330aff
https://doi.org/10.1101/2023.05.10.540130
https://doi.org/10.1101/2023.05.10.540130
Prolonged pausing of the transcription machinery may lead to the formation of three-stranded nucleic acid structures, called R-loops, typically resulting from the annealing of the nascent RNA with the template DNA. Unscheduled persistence of R-loops
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c4039699722361aa456d4182fbb33da1
https://doi.org/10.1101/2022.08.25.505353
https://doi.org/10.1101/2022.08.25.505353
Autor:
David Zapletal, Eliska Taborska, Josef Pasulka, Radek Malik, Karel Kubicek, Martina Zanova, Christian Much, Marek Sebesta, Valeria Buccheri, Filip Horvat, Irena Jenickova, Michaela Prochazkova, Jan Prochazka, Matyas Pinkas, Jiri Novacek, Diego F. Joseph, Radislav Sedlacek, Carrie Bernecky, Dónal O’Carroll, Richard Stefl, Petr Svoboda
Publikováno v:
Zapletal, D, Taborska, E, Pasulka, J, Malik, R, Kubicek, K, Zanova, M, Much, C, Sebesta, M, Buccheri, V, Horvat, F, Jenickova, I, Prochazkova, M, Prochazka, J, Pinkas, M, Novacek, J, Joseph, D F, Sedlacek, R, Bernecky, C, O'Carroll, D, Stefl, R & Svoboda, P 2022, ' Structural and functional basis of mammalian microRNA biogenesis by Dicer ', Molecular Cell, vol. 82, no. 21, pp. 4064-4079.e13 . https://doi.org/10.1016/j.molcel.2022.10.010
MicroRNA (miRNA) and RNA interference (RNAi) pathways rely on small RNAs produced by Dicer endonucleases. Mammalian Dicer primarily supports the essential gene-regulating miRNA pathway, but how it is specifically adapted to miRNA biogenesis is unknow
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6d7ba0dd8dca43acb8f9a0f66b38fecb
https://pubmed.ncbi.nlm.nih.gov/36332606
https://pubmed.ncbi.nlm.nih.gov/36332606