Výsledky vyhledávání - "Marek Sebela"

Akademický článek

Development of modern immunization agent against bovine papillomavirus type 1 infection based on BPV1 L1 recombinant protein

Autor: Alena Vrablikova, Veronika Brezani, Ivan Psikal, Martin Fraiberk, Marek Sebela, Martina Fojtikova, Pavel Kulich, Renata Hezova, Josef Masek

Publikováno v: Frontiers in Veterinary Science, Vol 10 (2023)

Bovine papillomavirus type 1 L1 protein was produced in a baculovirus expression system and purified as virus-like particles (VLPs) by affinity chromatography using lectins. The morphological integrity of VLPs was confirmed by electron microscopy. Di

Externí odkaz: https://doaj.org/article/59ced5e6048c4d7e9581edfbc5f2d570

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Akademický článek

The Position of His-Tag in Recombinant OspC and Application of Various Adjuvants Affects the Intensity and Quality of Specific Antibody Response after Immunization of Experimental Mice.

Autor: Michal Krupka, Josef Masek, Lucia Barkocziova, Pavlina Turanek Knotigova, Pavel Kulich, Jana Plockova, Robert Lukac, Eliska Bartheldyova, Stepan Koudelka, Radka Chaloupkova, Marek Sebela, Daniel Zyka, Ladislav Droz, Roman Effenberg, Miroslav Ledvina, Andrew D Miller, Jaroslav Turanek, Milan Raska

Publikováno v: PLoS ONE, Vol 11, Iss 2, p e0148497 (2016)

Lyme disease, Borrelia burgdorferi-caused infection, if not recognized and appropriately treated by antibiotics, may lead to chronic complications, thus stressing the need for protective vaccine development. The immune protection is mediated by phago

Externí odkaz: https://doaj.org/article/53b2fcf473fc4515a77e09ad8324db0c

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Biomolecular Profiling by MALDI-TOF Mass Spectrometry in Food and Beverage Analyses

Autor: Marek Sebela

Publikováno v: International Journal of Molecular Sciences. 23:13631

Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) has frequently been applied to the analysis of biomolecules. Its strength resides not only in compound identification but particularly in acquiring molecular

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fda6a357d9e6054f581c9c3bea54dd7b
https://doi.org/10.3390/ijms232113631

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Spoilage Detection with Biogenic Amine Biosensors, Comparison of Different Enzyme Electrodes

Autor: Beáta Bóka, Marek Sebela, Diána Virág, Attila Kiss, Nóra Adányi

Publikováno v: Electroanalysis. 24:181-186

Three enzyme based amperometric biosensors for biogenic amines were applied for meat spoilage monitoring. With diamine oxidase (EC 1.4.3.6), the total biogenic amine content was measured. Monoamine oxidase A (EC 1.4.3.4) was used for determination of

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::e0fe685ff04b75de445bacb44e2f984e
https://doi.org/10.1002/elan.201100419

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MALDI-based intact spore mass spectrometry of downy and powdery mildews

Autor: Jana, Chalupová, Michaela, Sedlářová, Michaela, Helmel, Pavel, Rehulka, Martina, Marchetti-Deschmann, Günter, Allmaier, Marek, Sebela

Publikováno v: Journal of mass spectrometry : JMS. 47(8)

Fast and easy identification of fungal phytopathogens is of great importance in agriculture. In this context, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) has emerged as a powerful tool for analyzing mic

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::897257528bec16e0c81aaa4aa3c626e5
https://pubmed.ncbi.nlm.nih.gov/22899506

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Isolation of recombinant cysteine dioxygenase protein from Trichophyton mentagrophytes

Autor: Alena, Kasperova, Jiri, Kunert, Milada, Horynova, Evzen, Weigl, Marek, Sebela, Rene, Lenobel, Milan, Raska

Publikováno v: Mycoses. 54(5)

Cysteine dioxygenase (CDO, EC 1.13.11.20) catalyses the oxygenation of cysteine to cysteine sulphinic acid leading to the production of sulphite, sulphate and taurine as the final metabolites of cysteine catabolism. Keratinolytic fungi secrete sulphi

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::6b0f2e3ebdede039b881d998abeeb821
https://pubmed.ncbi.nlm.nih.gov/21039937

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Identification of N-glycosylation in prolyl endoprotease from Aspergillus niger and evaluation of the enzyme for its possible application in proteomics

Autor: Marek, Sebela, Pavel, Rehulka, Jaromír, Kábrt, Helena, Rehulková, Tomás, Ozdian, Martin, Raus, Vojtech, Franc, Josef, Chmelík

Publikováno v: Journal of mass spectrometry : JMS. 44(11)

An acidic prolyl endoprotease from Aspergillus niger was isolated from the commercial product Brewers Clarex to evaluate its possible application in proteomics. The chromatographic purification yielded a single protein band in sodium dodecyl sulfate

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::4fa253983cd94aa0cd671fd8c8483f89
https://pubmed.ncbi.nlm.nih.gov/19757411

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