Biochemical, Biophysical, and Functional Analyses of Two Isoforms of the SnRK2 inhibitor AtSCS

Autor: Grażyna Goch, Maria Bucholc, Michal Dadlez, Jarosław Poznański, Halina Fedak, Grażyna Dobrowolska, Marcin Pierechod, Arkadiusz Ciesielski, Maria Klimecka, Małgorzata Lichocka, Anna Kulik, Krzysztof Tarnowski, Richard A. Engh

SNF1-related protein kinases 2 (SnRK2s) are key signaling elements that regulate abscisic acid (ABA)-dependent plant development and responses to environmental stresses. Our previous data showed that the SnRK2-interacting Calcium Sensor (SCS) is an i

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f35518cb9a1a34c32fec57cfe327bf3e

Opposing effects of DNA on proteolysis of a replication initiator

Autor: Katarzyna Wegrzyn, Igor Konieczny, Marcin Pierechod, Slawomir Kubik

Publikováno v: Nucleic Acids Research

DNA replication initiation proteins (Reps) are subjected to degradation by cellular proteases. We investigated how the formation of nucleoprotein complex, involving Rep and a protease, affects Rep degradation. All known Escherichia coli AAA+ cytosoli

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c85da5d2c1e5d00abe9f0d8665a9bd1f
http://europepmc.org/articles/PMC3273809

Coupling of transcription and replication machineries in λ DNA replication initiation: evidence for direct interaction of Escherichia coli RNA polymerase and the λO protein

Autor: Anna Szambowska, Marcin Pierechod, Grzegorz Węgrzyn, Monika Glinkowska

Publikováno v: Nucleic Acids Research

Transcription proceeding downstream of the λ phage replication origin was previously shown to support initial steps of the λ primosome assembly in vitro and to regulate frequency and directionality of λ DNA replication in vivo. In this report, the

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::933c61010c4cba2e13d625c7558c1467
https://pubmed.ncbi.nlm.nih.gov/20833633

Bacterial partitioning proteins affect the subcellular location of broad-host-range plasmid RK2

Autor: Igor Konieczny, Monika Witosinska, Katarzyna Kolatka, Marcin Pierechod

Publikováno v: Microbiology (Reading, England). 154(Pt 9)

It has been demonstrated that plasmids are not randomly distributed but are located symmetrically in mid-cell, or (1/4), (3/4) positions in bacterial cells. In this work we compared the localization of broad-host-range plasmid RK2 mini-replicons, whi

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https://pubmed.ncbi.nlm.nih.gov/18757818

Characterization of a cold-active and salt tolerant esterase identified by functional screening of Arctic metagenomic libraries

Autor: Nils Peder Willassen, Marcin Pierechod, Donatella de Pascale, Bjørn Altermark, Luca Ambrosino, Concetta De Santi

Publikováno v: BMC biochemistry (Online) (2016). doi:10.1186/s12858-016-0057-x
info:cnr-pdr/source/autori:De Santi C.; Altermark B.; Pierechod M.M.; Ambrosino L.; de Pascale D.; Willassen N.-P./titolo:Characterization of a cold-active and salt tolerant esterase identified by functional screening of Arctic metagenomic libraries/doi:10.1186%2Fs12858-016-0057-x/rivista:BMC biochemistry (Online)/anno:2016/pagina_da:/pagina_a:/intervallo_pagine:/volume
BMC Biochemistry

Background The use of metagenomics in enzyme discovery constitutes a powerful approach to access to genomes of unculturable community of microorganisms and isolate novel valuable biocatalysts for use in a wide range of biotechnological and pharmaceut

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b9e373581bddc9ccfcebf8f323633bba