Large-scale profiling of noncoding RNA function in yeast.

Autor: Steven Parker, Marcin G Fraczek, Jian Wu, Sara Shamsah, Alkisti Manousaki, Kobchai Dungrattanalert, Rogerio Alves de Almeida, Edith Invernizzi, Tim Burgis, Walid Omara, Sam Griffiths-Jones, Daniela Delneri, Raymond T O'Keefe

Publikováno v: PLoS Genetics, Vol 14, Iss 3, p e1007253 (2018)

Noncoding RNAs (ncRNAs) are emerging as key regulators of cellular function. We have exploited the recently developed barcoded ncRNA gene deletion strain collections in the yeast Saccharomyces cerevisiae to investigate the numerous ncRNAs in yeast wi

Externí odkaz: https://doaj.org/article/8b35311aa8174fb69cbdc7632eb1f55c

Genome-wide analysis of the non-coding RNA synthetic genetic network reveals extensive plasticity and unique phenotypes in yeast

Autor: Marcin G Fraczek, Soukaina Timouma, Laura Natalia Balarezo-Cisneros, Steven Parker, Kobchai Duangrattanalert, Matthew Wenjie Feng, Sam Griffiths-Jones, Raymond T O’Keefe, Daniela Delneri

Recent studies identified non-coding RNAs (ncRNAs) with unknown function that are responsible for major fitness changes in yeast. To understand ncRNA interplay and aid their functional assignment, the synthetic genetic array (SGA) methodology was emp

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::d7092db67e14da584c56508babbe39a4
https://doi.org/10.1101/2022.03.10.483001

Functional and transcriptional profiling of non-coding RNAs in yeast reveal context-dependent phenotypes and in trans effects on the protein regulatory network

Autor: Daniela Delneri, Soukaina Timouma, Marcin G. Fraczek, Raymond T. O'Keefe, Catherine B. Millar, Steven Parker, Ping Wang, Laura Natalia Balarezo-Cisneros

Publikováno v: Balarezo-Cisneros, L N, Parker, S, Fraczek, M G, Timouma, S, Wang, P, O'Keefe, R T, Millar, C B & Delneri, D 2021, ' Functional and transcriptional profiling of non-coding RNAs in yeast reveal context-dependent phenotypes and in trans effects on the protein regulatory network ', PLoS Genetics, vol. 17, no. 1, e1008761 . https://doi.org/10.1371/journal.pgen.1008761
PLoS Genetics
PLoS Genetics, Vol 17, Iss 1, p e1008761 (2021)

Non-coding RNAs (ncRNAs), including the more recently identified Stable Unannotated Transcripts (SUTs) and Cryptic Unstable Transcripts (CUTs), are increasingly being shown to play pivotal roles in the transcriptional and post-transcriptional regulat

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5229b68a60ccbb802158bf2ff091d9f2
https://www.research.manchester.ac.uk/portal/en/publications/functional-and-transcriptional-profiling-of-noncoding-rnas-in-yeast-reveal-contextdependent-phenotypes-and-in-trans-effects-on-the-protein-regulatory-network(8b9cfaa5-7364-41ad-beaa-a805b5d78d30).html

Corticosteroid treatment is associated with increased filamentous fungal burden in allergic fungal disease

Autor: Lucy J.C. Smyth, Marcin G. Fraczek, Livingstone Chishimba, David W. Denning, Michael Bromley, Paul Bowyer, Robert Niven, Angela Simpson

Publikováno v: Fraczek, M G, Chishimba, L, Niven, R M, Bromley, M, Simpson, A, Smyth, L, Denning, D W & Bowyer, P 2018, ' Corticosteroid treatment is associated with increased filamentous fungal burden in allergic fungal disease ', The Journal of allergy and clinical immunology . https://doi.org/10.1016/j.jaci.2017.09.039

Background\ud Allergic diseases caused by fungi are common. The best understood conditions are allergic\ud bronchopulmonaryaspergillosis (ABPA) and severe asthma with fungal sensitisation (SAFS).\ud Our knowledge of the fungal microbiome (mycobiome)

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d62e839bef61c12402b91e05486804d9
https://doi.org/10.1016/j.jaci.2017.09.039

Fast and Reliable PCR Amplification fromAspergillus fumigatusSpore Suspension Without Traditional DNA Extraction

Autor: Paul Bowyer, Lauren Dineen, Marcin G. Fraczek, Can Zhao, Daniela Delneri, Ressa Lebedinec, Michael Bromley

Publikováno v: Current Protocols in Microbiology
Fraczek, M, Zhao, C, Dineen, L, Lebedinec, R, Bowyer, P, Bromley, M & Delneri, D 2019, ' Fast and reliable PCR amplification from Aspergillus fumigatus spore suspension without traditional DNA extraction ', Current Protocols in Microbiology . https://doi.org/10.1002/cpmc.89

Aspergillus fumigatus is an opportunistic human pathogenic mold. DNA extraction from this fungus is usually performed by mechanical perturbation of cells, as it possesses a rigid and complex cell wall. While this is not problematic for single isolate

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::89e57f39519d9bf8c30ccc205044e404
https://doi.org/10.1002/cpmc.89