Zobrazeno 1 - 10
of 74
pro vyhledávání: '"Marcia J. Holden"'
Publikováno v:
Expert Review of Molecular Diagnostics. 11:741-755
There is a great need for harmonization in nucleic acid testing for infectious disease and clinical genetics. The proliferation of assay methods, the number of targets for molecular diagnostics and the absence of standard reference materials contribu
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::48236b68164ed0a522915a3759fac480
https://doi.org/10.1586/erm.11.50
https://doi.org/10.1586/erm.11.50
Publikováno v:
Food Control. 20:391-401
Isogenic NK603 DNA was degraded by sonication or heat and quantified using A260 and two fluorescent dye methods. Quantitative PCR (qPCR) experiments were conducted by amplifying an SSIIb-3 endogenous control and an NK603 transgene in untreated, sonic
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::e5af632d0c0857a0d8d2fe93aa7969e3
https://doi.org/10.1016/j.foodcont.2008.07.009
https://doi.org/10.1016/j.foodcont.2008.07.009
Publikováno v:
Biochemistry. 46:13235-13244
Putidaredoxin reductase (PdR) is the flavin protein that carries out the first electron transfer involved in the cytochrome P450cam catalytic cycle. In PdR, the flavin adenine dinucleotide (FAD/FADH2) redox center acts as a transformer by accepting t
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5b915d790ad3c95c9aaa93f1d2c07018
https://doi.org/10.1021/bi7012118
https://doi.org/10.1021/bi7012118
Autor:
Malcolm Burns, David Gancberg, Marcia J. Holden, Maxim S Vonsky, Jing Wang, Philippe Corbisier, Qing Yan Liu, Koichi Chiba, Tomoya Okunishi, Lina Partis, Kerry R. Emslie, Inchul Yang
Publikováno v:
ELECTROPHORESIS. 28:3193-3200
A simple amplified fragment length polymorphism (AFLP) model, using the bacteriophage lambda genome, was developed to test the reproducibility of this technique in an international comparative study. Using either non-selective or selective primers, n
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ffe81fb1ab85e65d10887dfee6dcfd4c
https://doi.org/10.1002/elps.200700247
https://doi.org/10.1002/elps.200700247
Autor:
Anna Baoutina, X Della Sin, Wim Broothaerts, Marcia J. Holden, Satoshi Furui, Malcolm Burns, Mamoru Kawaharasaki, Kerry R. Emslie, Sabrina Gioria, Jing Wang, Heinz Schimmel, Yun-Mi Lee, Yasunori Kurosawa, Hyong-Ha Kim, Philippe Corbisier
Publikováno v:
Journal of Agricultural and Food Chemistry. 55:3249-3257
An international CCQM-P60 pilot study involving eight national metrological institutes was organized to investigate if the quantification of genetically modified (GM) corn powder by real-time PCR was affected by the DNA extraction method applied. Fou
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::aa4c5cfe99de6ab69d55bca685bf8688
https://doi.org/10.1021/jf062931l
https://doi.org/10.1021/jf062931l
Publikováno v:
Analytical Chemistry. 79:1536-1541
Measurement of the phosphorus content of nucleotides and deoxyribonucleic acid (DNA) offers an approach to the quantitation of nucleic acids that is traceable to the SI. Such measurements can be an alternative to the commonly used spectroscopic tools
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d0e0b7123864200df56322df96a5ef0b
https://doi.org/10.1021/ac061463b
https://doi.org/10.1021/ac061463b
Autor:
Martin P. Mayhew, Andrew Howard, David T. Gallagher, Salita Kaistha, Natasha Smith, Adrian E. Roitberg, Eva Rivera, Marcia J. Holden
Publikováno v:
Archives of Biochemistry and Biophysics. 445:72-80
Chorismate lyase (CL) removes the pyruvyl group from chorismate to provide 4-hydroxybenzoate (4HB) for the ubiquinone pathway. We previously reported the crystal structure at 1.4 A resolution of the Escherichia coli CL with bound 4HB product, showing
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b6d077120d78e6c1caa50032571adc2e
https://doi.org/10.1016/j.abb.2005.10.026
https://doi.org/10.1016/j.abb.2005.10.026
Autor:
David T. Gallagher, Harold G. Monbouquette, Imke Schröder, Hugh Robinson, Natasha Smith, Marcia J. Holden
Publikováno v:
Journal of Molecular Biology. 342:119-130
The hyperthermophilic archaeon Archaeoglobus fulgidus contains an l-Ala dehydrogenase (AlaDH, EC 1.4.1.1) that is not homologous to known bacterial dehydrogenases and appears to represent a previously unrecognized archaeal group of NAD-dependent dehy
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fae2c148b078c6b2ea3074a0907346c0
https://doi.org/10.1016/j.jmb.2004.06.090
https://doi.org/10.1016/j.jmb.2004.06.090
Autor:
David T. Gallagher, Martin P. Mayhew, Annie Heroux, Natasha Smith, David Charlton, Marcia J. Holden, Howard Robinson
Publikováno v:
Acta Crystallographica Section D Biological Crystallography. 59:2328-2331
Alanine dehydrogenase (AlaDH) from the hyperthermophilic archaeon Archaeoglobus fulgidus is a dimer of 35 kDa chains. The archaeal enzyme appears to represent a new class of AlaDH that is not homologous to bacterial AlaDH enzymes, but has close evolu
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::59400caa0baf4ce57ecc266c54e6608c
https://doi.org/10.1107/s0907444903021565
https://doi.org/10.1107/s0907444903021565
Autor:
Joseph R. Blasic, Donald C. Kendall, Luke A. Shokere, G. Ronald Jenkins, Chuan Kao, Louis Bussjaeger, Marcia J. Holden, Larry Freese
Publikováno v:
Journal of Agricultural and Food Chemistry. 51:2468-2474
Sensitive and accurate testing for trace amounts of biotechnology-derived DNA from plant material requires pure, high-quality genomic DNA as template for subsequent amplification using the polymerase chain reaction (PCR). Six methodologies were evalu
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::78207b7a084fed7b1bf6d28cbb602850
https://doi.org/10.1021/jf0211130
https://doi.org/10.1021/jf0211130