Zobrazeno 1 - 8
of 8
pro vyhledávání: '"Marcelle Tuttle"'
Autor:
Angela Tung, Denitsa Milanova, Sumana Sharma, Alissa Lance-Byrne, Alejandro Chavez, Samira Kiani, Mo R. Ebrahimkhani, Nan Cher Yeo, Ryan J Cecchi, Xiaoge Guo, James J. Collins, Noah Davidsohn, David J. Menn, Marcelle Tuttle, Elaine T. Lim, Yingleong Chan, Chih-Chung Kuo, Nathan E. Lewis, George M. Church, Swechchha Pradhan
Publikováno v:
Nature methods
The RNA-guided endonuclease Cas9 can be converted into a programmable transcriptional repressor, but inefficiencies in target-gene silencing have limited its utility. Here we describe an improved Cas9 repressor based on the C-terminal fusion of a rat
Autor:
Alejandro Chavez, Marcelle Tuttle, Brian M. Turczyk, George M. Church, Benjamin W. Pruitt, Michael Tung, Rebecca S. Shapiro, Jordan Winston, Ryan J Cecchi, James J. Collins
Publikováno v:
Proceedings of the National Academy of Sciences. 115:3669-3673
Here, we present a generalized method of guide RNA "tuning" that enables Cas9 to discriminate between two target sites that differ by a single-nucleotide polymorphism. We employ our methodology to generate an in vivo mutation prevention system in whi
Autor:
Benjamin E. Housden, Alejandro Chavez, Ben Ewen-Campen, Emma J. K. Kowal, Dmitry Ter-Ovanesyan, Norbert Perrimon, George M. Church, James J. Collins, Ryan J Cecchi, Benjamin W. Pruitt, Sabina J Haque, Joanna Buchthal, Raj Chari, Marcelle Tuttle
Publikováno v:
Nature Methods. 13:563-567
Several programmable transcription factors exist based on the versatile Cas9 protein, yet their relative potency and effectiveness across various cell types and species remain unexplored. Here, we compare Cas9 activator systems and examine their abil
Autor:
George M. Church, Richard N. Hall, Benjamin W. Pruitt, Suhani Vora, Mohammad R. Ebrahimkhani, Dmitry Ter-Ovanesyan, Jacob Beal, Alejandro Chavez, Samira Kiani, Ron Weiss, Emma J. K. Kowal, James J. Collins, Marcelle Tuttle, Joanna Buchthal, Jason Qian, Raj Chari
Publikováno v:
PMC
We demonstrate that by altering the length of Cas9-associated guide RNA(gRNA) we were able to control Cas9 nuclease activity and simultaneously perform genome editing and transcriptional regulation with a single Cas9 protein. We exploited these princ
Autor:
Jonathan L. Braff, John Aach, Norbert Perrimon, Dmitry Ter-Ovanesyan, Benjamin E. Housden, Samira Kiani, Daniel J. Wiegand, Christopher D. Guzman, Shuailiang Lin, Jonathan Scheiman, James J. Collins, Eswar Prasad Ramachandran Iyer, Noah Davidsohn, Alejandro Chavez, Ron Weiss, George M. Church, Benjamin W. Pruitt, Suhani Vora, Marcelle Tuttle
Publikováno v:
Nature methods
The RNA-guided nuclease Cas9 can be reengineered as a programmable transcription factor. However, modest levels of gene activation have limited potential applications. We describe an improved transcriptional regulator obtained through the rational de
Autor:
Ryan J Cecchi, Rebecca S. Shapiro, James J. Collins, Jordan Winston, Alejandro Chavez, Marcelle Tuttle, Michael Tung, George M. Church, Benjamin W. Pruitt, Brian M. Turczyk
Here we present a generalized method of guide RNA “tuning” that enables Cas9 to discriminate between two target sites that differ by a single nucleotide polymorphism. We employ our methodology to generate a novelin vivomutation prevention system
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fafedb990d1ca634964d9d8ee7120c53
Publikováno v:
The FEBS journal. 283(17)
Clustered regularly interspaced short palindromic repeats (CRISPRs) and CRISPR-associated (Cas) proteins offer a breakthrough platform for cheap, programmable, and effective sequence-specific DNA targeting. The CRISPR-Cas system is naturally equipped
Autor:
John Aach, Marcelle Tuttle, Benjamin W. Pruitt, Christopher D. Guzman, Daniel J. Wiegand, James J. Collins, Jonathan L. Braff, Suhani Vora, Samira Kiani, George M. Church, Noah Davidsohn, Jonathan Scheiman, Eswar Prasad Ramachandran Iyer, Alejandro Chavez, Ron Weiss, Dmitry Ter-Ovanesyan
The RNA-guided bacterial nuclease Cas9 can be reengineered as a programmable transcription factor by a series of changes to the Cas9 protein in addition to the fusion of a transcriptional activation domain (AD). However, the modest levels of gene act
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b342c6ffa1672d722f7eba65570c1048