Zobrazeno 1 - 10
of 11
pro vyhledávání: '"Marcella A. Gilmore"'
Publikováno v:
Journal of the American Chemical Society. 119:4388-4393
An investigation of 9-ethyladenine (9-EA) imprinted polymers has provided insight into the origins of binding and recognition by imprinted polymers. Porogens and polymerization temperature are impo...
Autor:
J. B. Alexander Ross,‡ and, A. Richard Chamberlin, Justin E. Carlson, Lance E. Steward, Marcella A. Gilmore, Cynthia S. Collins
Publikováno v:
Journal of the American Chemical Society. 119:6-11
Fluorescence spectroscopy is a powerful biophysical technique for studying protein structure, function, dynamics, and intermolecular interactions. Such studies are often conducted using intrinsic probes, such as tryptophan residues, or extrinsic prob
Autor:
Lance E. Steward, Justin A. Ross, Marcella A. Gilmore, Dudley H. Williams, K. Roger Aoki, David M. Jameson
Publikováno v:
Analytical biochemistry. 413(1)
Our previous article described a fluorescence-based assay for monitoring the proteolytic activity of botulinum neurotoxin types A and E (BoNT/A and BoNT/E). As detailed in that article, the assay is based on depolarization due to Forster resonance en
Autor:
Dudley H. Williams, Justin A. Ross, Yumiko Okawa, K. Roger Aoki, Marcella A. Gilmore, B. Holguin, Nicholas G. James, Lance E. Steward, David M. Jameson
Publikováno v:
Analytical biochemistry. 413(1)
The DARET (depolarization after resonance energy transfer) assay is a coupled Förster resonance energy transfer (FRET)-fluorescence polarization assay for botulinum neurotoxin type A or E (BoNT/A or BoNT/E) proteolytic activity that relies on a full
Autor:
Patton E. Garay, Ester Fernandez-Salas, Marcella A. Gilmore, Helen Ho, Sarah W. Sun, K. Roger Aoki, Lance E. Steward, Joanne Wang, Joseph V. Ordas, Joseph Francis
Publikováno v:
Proceedings of the National Academy of Sciences of the United States of America. 101(9)
Botulinum neurotoxin (BoNT) is a potent biological substance used to treat neuromuscular and pain disorders. Both BoNT type A and BoNT type E display high-affinity uptake into motor neurons and inhibit exocytosis through cleavage of the synaptosome-a
Publikováno v:
Implementation and Redesign of Catalytic Function in Biopolymers ISBN: 9783540657286
The method of site-specific mutagenesis with noncoded amino acids using suppression of a nonsense codon by a semi-synthetic tRNA was first introduced in 1989. Initially used to probe the tolerance of the protein biosynthetic machinery for compounds o
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::dc3d8faea16f62af55a1150d3e8acf5f
https://doi.org/10.1007/3-540-48990-8_3
https://doi.org/10.1007/3-540-48990-8_3
Autor:
Nicholson Gregory, Fernandez-Salas Ester, Brown Meenakshi, Okawa Yumiko, Francis Joseph, Sun Sarah, Lance E. Steward, K. Roger Aoki, Hieu T. Arpawong, Satorius Alan, Maria C. Ardila, Joseph V. Ordas, Wang Joanne, Marcella A. Gilmore
Publikováno v:
Toxicon. 51:11-12
Both rHis6-BoNT/E and rBoNT/E-His6 were produced from a codon optimized gene within Escherichia coli. Similar to native BoNT/E isolated from Clostridium botulinum, the recombinant proteins were isolated as single-chain molecules. The His6 affinity ta
Autor:
Roger K. Aoki, Dudley J. Williams, Nicholas G. James, Marcella A. Gilmore, Lance E. Steward, David M. Jameson, Justin A. Ross
Publikováno v:
Biophysical Journal. 98:584a
The assay is based on depolarization due to Forster Resonance Energy Transfer (FRET) between Blue Fluorescent Protein (BFP) and Green Fluorescent Protein (GFP) moieties linked by a peptide containing residues 134-206 of SNAP-25, the protein substrate
Publikováno v:
Neurotoxicity Research; 2006, Vol. 9 Issue 2/3, p217-224, 8p
Autor:
Mariana Chilton
A radical and urgent new approach to how we can solve the problems of hunger and poverty in the US.Most people think hunger has to do with food: researchers, policymakers, and advocates focus on promoting government-funded nutrition assistance; well-