Time-Lapse Flow Cytometry: A Robust Tool to Assess Physiological Parameters Related to the Fertilizing Capability of Human Sperm

Autor: Paulina Torres-Rodríguez, Arturo Matamoros-Volante, Claudia L. Treviño, Marcela B. Treviño, Valeria Castillo-Viveros

Publikováno v: International Journal of Molecular Sciences, Vol 22, Iss 93, p 93 (2021)
International Journal of Molecular Sciences
Volume 22
Issue 1

Plasma membrane (PM) hyperpolarization, increased intracellular pH (pHi), and changes in intracellular calcium concentration ([Ca2+]i) are physiological events that occur during human sperm capacitation. These parameters are potential predictors of s

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1700885eb164c2cdb99d700ef348fe42
https://www.mdpi.com/1422-0067/22/1/93

Three Drought-Responsive Members of the Nonspecific Lipid-Transfer Protein Gene Family in Lycopersicon pennelliiShow Different Developmental Patterns of Expression1

Autor: Marcela B. Treviño, Mary A. O' Connell

Publikováno v: Plant Physiology. 116:1461-1468

Genomic clones of two nonspecific lipid-transfer protein genes from a drought-tolerant wild species of tomato (Lycopersicon pennellii Corr.) were isolated using as a probe a drought- and abscisic acid (ABA)-induced cDNA clone (pLE16) from cultivated

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::7db3e795ede105d7f658e7de034346a5
https://doi.org/10.1104/pp.116.4.1461

Measuring Intracellular Ca2+ Changes in Human Sperm using Four Techniques: Conventional Fluorometry, Stopped Flow Fluorometry, Flow Cytometry and Single Cell Imaging

Autor: Claudia L. Treviño, Omar José, Paulina Torres-Rodríguez, Marcela B. Treviño, Ana A. Sánchez-Tusie, Yoloxochitl Sánchez-Guevara, Esperanza Mata-Martínez, Alejandra Solís-López

Publikováno v: Journal of Visualized Experiments.

Spermatozoa are male reproductive cells especially designed to reach, recognize and fuse with the egg. To perform these tasks, sperm cells must be prepared to face a constantly changing environment and to overcome several physical barriers. Being in

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::06eeac0a6e21f8bfdb3b99cf560a7fc5
https://doi.org/10.3791/50344-v