An accurate real-time PCR test for the detection and quantification of cauliflower mosaïc virus (CaMV): applicable in GMO screening

Autor: C. Kerlan, Marie Noelle Fortabat, Yves Bertheau, Maher Chaouachi, Pierre Yot, Angèle Geldreich, Colette Audeon, Naïma Kebdani, Marcel Romaniuk

Publikováno v: European Food Research and Technology
European Food Research and Technology, Springer Verlag (Germany), 2008, 227 (03), pp.789-798. ⟨10.1007/s00217-007-0787-5⟩
European Food Research and Technology, 2008, 227 (03), pp.789-798. ⟨10.1007/s00217-007-0787-5⟩

International audience; Due to its very large use in the first generation of genetically modified organisms (GMO), the 35S promoter derived from the cauliflower mosaic virus (CaMV) is the most used PCR target for screening tests in GMO routine analys

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b95adebbd956962b9e77292d5122fe93
https://doi.org/10.1007/s00217-007-0787-5

Characterization and Event Specific-Detection by Quantitative Real-Time PCR of T25 Maize Insert

Autor: Sophie Fernandez, Pieter Windels, Géraldine Coué-Philippe, Cécile Collonnier, Annick Diolez, Marc de Beuckeleer, Marc De Loose, André Kobilinsky, Yves Bertheau, Alexandra Schattner, Marie-Noëlle Duplan, Naïma Kebdani, Marcel Romaniuk, Francine Boyer, Georges Berthier

Publikováno v: Journal of AOAC INTERNATIONAL
Journal of AOAC INTERNATIONAL, AOAC International, 2005, 88 (2), pp.536-546
Scopus-Elsevier
HAL

T25 is one of the 4 maize transformation events from which commercial lines have so far been authorized in Europe. It was created by polyethylene glycol-mediated transformation using a construct bearing one copy of the synthetic pat gene associated w

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4c93baee415e3380798e44ce30205088
https://doi.org/10.1093/jaoac/88.2.536

Development of real-time PCR method for the detection and the quantification of a new endogenous reference gene in sugar beet 'Beta vulgaris L.': GMO application

Autor: Nesrine Nabi, Maher Chaouachi, Akram Alaya, Marcel Romaniuk, Ahmed Ben Hafsa, Aurélie Bérard, Fethia Skhiri, Khaled Said, Imen Ben Haj Ali

Publikováno v: Plant Cell Reports
Plant Cell Reports, Springer Verlag, 2013, 32 (1), pp.117-128. ⟨10.1007/s00299-012-1346-5⟩

KEY MESSAGE : Here, we describe a new developed quantitative real-time PCR method for the detection and quantification of a new specific endogenous reference gene used in GMO analysis. The key requirement of this study was the identification of a new

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e1c9367347bbe1607daee0b97175cfe0
https://hal.inrae.fr/hal-02644567

The SNPlex technology : a high throughput method for the detection of authorized and unauthorized GMO with the matrix approach

Autor: Maher Chaouachi, Marcel Romaniuk, Aghleb Bartegi, Valerie Laval, Yves Bertheau, Brunel, Dominique D.

Publikováno v: 3. International Conference on Coexistence between Genetically Modified (GM) and non-GM based agricultural Supply Chains
3. International Conference on Coexistence between Genetically Modified (GM) and non-GM based agricultural Supply Chains, Nov 2007, Séville, Spain
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International audience

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=dedup_wf_001::9fecaff30dcafd6a16750d4ca46a9d27
https://hal.inrae.fr/hal-02751361

A high-throughput multiplex method adapted for GMO detection

Autor: Gaelle Chupeau, Yves Bertheau, Heather I. McKhann, Marcel Romaniuk, Valérie Laval, Sandra Giancola, Maher Chaouachi, Dominique Brunel, Aurélie Bérard

Publikováno v: Journal of Agricultural and Food Chemistry
Journal of Agricultural and Food Chemistry, 2008, 56 (24), pp.11596-11606. ⟨10.1021/jf801482r⟩
Journal of Agricultural and Food Chemistry, American Chemical Society, 2008, 56 (24), pp.11596-11606. ⟨10.1021/jf801482r⟩

International audience; A high-throughput multiplex assay for the detection of genetically modified organisms (GMO) was developed on the basis of the existing SNPlex method designed for SNP genotyping. This SNPlex assay allows the simultaneous detect

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2043511ad1fcb72552edbc4e040c38f4
https://hal.inrae.fr/hal-02664782