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pro vyhledávání: '"Marc K. J. J. Moeremans"'
Autor:
Marc K. J. J. Moeremans, Petrus J. Pauwels, Harrie P. van Assouw, Josée E. Leysen, Luc Peeters
Publikováno v:
Synapse. 12:271-280
The neuroprotective properties of the cognitive enhancer sabeluzole were investigated in rat brain neuronal cultures derived from the hippocampal formation of 17-day-old rat embryos. Measurement of the neuronal cytoskeletal microtubule-associated pro
Autor:
Alan Jones, Marc K. J. J. Moeremans
Immunoblotting techniques, in which electrophoretically separated proteins are transferred to the surface of nitrocellulose or nylon membranes ("Western" blots), are becoming increasingly popular in the analysis of protein systems. Transfer technique
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::e7b9f6de96c35edffe2e380606b47f4d
https://doi.org/10.1385/0-89603-126-8:441
https://doi.org/10.1385/0-89603-126-8:441
Publikováno v:
Analytical Biochemistry. 153:18-22
A staining method for proteins on (positively charged) nylon and nitrocellulose membranes is described. The two-step method uses cationic cacodylate iron colloid which is substituted with Tween 20 at an OD460 nm = 0.5, followed by Perls' reaction wit
Autor:
C. Kinet-Denoel, Pierre G. Coulie, Léon J. Simar, Michel Y Braun, N. Cormann, Marc K. J. J. Moeremans, Jacques Van Snick, Ernst Heinen
Publikováno v:
European Journal of Immunology, 16 (2
Antigens in the form of immune complexes are retained on the membranes of follicular dendritic cells (FDC) for long periods of time. To examine how immune complexes reach germinal centers, where FDC are located, we injected mice with anti-2,4-dinitro
Publikováno v:
Electrophoresis. 8:403-409
Two procedures are described for staining the overall protein pattern on transfer membranes. Both methods involve the use of colloidal metal particles. AuroDye staining is based on the interaction of anionic colloidal gold particles with cationic pro
Publikováno v:
Journal of Immunological Methods. 89:89-91
A double staining method is described which combines immunodetection with sensitive staining of the complete electropherogram on the same membrane. The method is based on the use of Tween 20 as blocking agent, and uses immunogold/silver staining of s
Publikováno v:
Cell biology international reports. 5(9)
A new, simple procedure is described for the production of 5 nm colloidal gold/secondary antibody reagents. Utilizing them with antitubulin shows 1) that they can be used for high resolution ultrastructural localization studies and 2) that this can b
Publikováno v:
SPIE Proceedings.
A new technique for the visualization of small molecular components in living cells is presented. It is based on the exploitation, by means of video techniques of the slight diffraction power of small metal particles (20-40 nm) in the light microscop