Zobrazeno 1 - 10
of 122
pro vyhledávání: '"Marc, Ostermeier"'
Publikováno v:
PLoS ONE, Vol 17, Iss 6 (2022)
The engineering of switchable or activatable dCas9 proteins would benefit from a single system for both positive and negative selection of dCas9 activity. Most systems that are used to interrogate dCas9 libraries use a fluorescent protein screen or a
Externí odkaz:
https://doaj.org/article/f1a1e22722ad4d6689dca5d1901662aa
Publikováno v:
The CRISPR Journal. 6:43-51
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::38397772ab6518a171cf1fd967ed741d
https://doi.org/10.1089/crispr.2022.0041
https://doi.org/10.1089/crispr.2022.0041
Autor:
Tina Xiong, Glenna E. Meister, Rachael E. Workman, Nathaniel C. Kato, Michael J. Spellberg, Fulya Turker, Winston Timp, Marc Ostermeier, Carl D. Novina
Publikováno v:
Scientific Reports, Vol 7, Iss 1, Pp 1-14 (2017)
Abstract Mammalian genomes exhibit complex patterns of gene expression regulated, in part, by DNA methylation. The advent of engineered DNA methyltransferases (MTases) to target DNA methylation to specific sites in the genome will accelerate many are
Externí odkaz:
https://doaj.org/article/0f956c0b5d804d3494852eec91096851
Autor:
Liliane F. C. Ribeiro, Cynthia Chelius, Karthik R. Boppidi, Nisha S. Naik, Simin Hossain, Jessica J. J. Ramsey, Jyothi Kumar, Lucas F. Ribeiro, Marc Ostermeier, Bao Tran, Young Ah Goo, Leandro J. de Assis, Mevlut Ulas, Ozgur Bayram, Gustavo H. Goldman, Stephen Lincoln, Ranjan Srivastava, Steven D. Harris, Mark R. Marten
Publikováno v:
mBio, Vol 10, Iss 2 (2019)
ABSTRACT In filamentous fungi, an important kinase responsible for adaptation to changes in available nutrients is cyclic AMP (cAMP)-dependent protein kinase (protein kinase A [PKA]). This kinase has been well characterized at a molecular level, but
Externí odkaz:
https://doaj.org/article/9ee07479ba274071800d77d21b2bee63
Autor:
Tina Xiong, Dahlia Rohm, Rachael E Workman, Lauren Roundtree, Carl D Novina, Winston Timp, Marc Ostermeier
Publikováno v:
PLoS ONE, Vol 13, Iss 12, p e0209408 (2018)
Mammalian gene expression is a complex process regulated in part by CpG methylation. The ability to target methylation for de novo gene regulation could have therapeutic and research applications. We have previously developed a dCas9-MC/MN protein fo
Externí odkaz:
https://doaj.org/article/65e27a1bee884baf872e33c2213dccdc
Publikováno v:
The CRISPR journal.
Ribonuclease III (RNase III) and RNase III-like ribonucleases have a wide range of important functions and are found in all organisms, yet a simple and high-throughput
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::b1c70aef4f3cdd6db2b890ee89501143
https://pubmed.ncbi.nlm.nih.gov/36493370
https://pubmed.ncbi.nlm.nih.gov/36493370
Autor:
Marc Ostermeier, Ryan Weeks
How protein properties such as protein activity and protein essentiality affect the distribution of fitness effects (DFE) of mutations are important questions in protein evolution. Deep mutational scanning studies typically measure the effects of a c
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ff70453d9dc38a653e07c5ffae87853c
https://doi.org/10.1101/2022.11.01.514689
https://doi.org/10.1101/2022.11.01.514689
Autor:
Marc Ostermeier, Jacob Mehlhoff
Mutations can have deleterious fitness effects when they decrease protein specific activity or decrease active protein abundance. Mutations will also be deleterious when they cause misfolding or misinteractions that are toxic to the cell (i.e., indep
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::8ee656757db8b9a8af67cedc20d13b57
https://doi.org/10.1101/2022.10.24.513589
https://doi.org/10.1101/2022.10.24.513589
Publikováno v:
PLoS ONE, Vol 11, Iss 9, p e0162921 (2016)
Engineering heterologous nucleoside kinases inside E. coli is a difficult process due to the integral role nucleosides play in cell division and transcription. Nucleoside analogs are used in many kinase screens that depend on cellular metabolization
Externí odkaz:
https://doaj.org/article/c1f22db805394b6a97fd14bb3402ceb7
Autor:
Shaun Spisak, Marc Ostermeier
Publikováno v:
Biochemical Society Transactions. 48:2205-2212
There is an ongoing need in the synthetic biology community for novel ways to regulate gene expression. Protein switches, which sense biological inputs and respond with functional outputs, represent one way to meet this need. Despite the fact that th
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8de07a8ad1c2cc4658819fb996f9be19
https://doi.org/10.1042/bst20200441
https://doi.org/10.1042/bst20200441