Zobrazeno 1 - 10
of 29
pro vyhledávání: '"María Mercedes Segura"'
Autor:
Ferran Tarrés-Freixas, Carmen Aguilar-Gurrieri, María Luisa Rodríguez de la Concepción, Victor Urrea, Benjamin Trinité, Raquel Ortiz, Edwards Pradenas, Pau Blanco, Sílvia Marfil, Luis Manuel Molinos-Albert, Ana Barajas, Anna Pons-Grífols, Carlos Ávila-Nieto, Ismael Varela, Laura Cervera, Sònia Gutiérrez-Granados, María Mercedes Segura, Francesc Gòdia, Bonaventura Clotet, Jorge Carrillo, Julià Blanco
Publikováno v:
npj Vaccines, Vol 8, Iss 1, Pp 1-13 (2023)
Abstract Antigen display on the surface of Virus-Like Particles (VLPs) improves immunogenicity compared to soluble proteins. We hypothesised that immune responses can be further improved by increasing the antigen density on the surface of VLPs. In th
Externí odkaz:
https://doaj.org/article/20b68eeb7d6f4ecfa2c0a0005799608d
Publikováno v:
PLoS ONE, Vol 7, Iss 7, p e42073 (2012)
The human adenovirus 40 (Ad40) is a promising tool for gene therapy of intestinal diseases. Since the production of Ad40 in vitro is extremely inefficient, chimeric Adenovirus 5/40S vectors carrying the Ad40 short fiber on the Ad5 capsid have been de
Externí odkaz:
https://doaj.org/article/85e73b1e1afc424f8a910e09ad795542
Publikováno v:
Recercat: Dipósit de la Recerca de Catalunya
Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
BMC Proceedings
BMC Proceedings, Vol 5, Iss Suppl 8, p P126 (2011)
Dipòsit Digital de Documents de la UAB
Universitat Autònoma de Barcelona
Recercat. Dipósit de la Recerca de Catalunya
instname
Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
BMC Proceedings
BMC Proceedings, Vol 5, Iss Suppl 8, p P126 (2011)
Dipòsit Digital de Documents de la UAB
Universitat Autònoma de Barcelona
Recercat. Dipósit de la Recerca de Catalunya
instname
Background Mammalian cells are a widely used expression platform for the production of recombinant therapeutic proteins or viral particle-based vaccines since they typically perform appropriate protein post-translational modifications and authentic v
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ad7b24cebfb71f5c17bb35157ece85ca
http://hdl.handle.net/2072/426472
http://hdl.handle.net/2072/426472
Publikováno v:
Biotechnology and Bioengineering. 114:2507-2517
Transient transfection is a fast, flexible, and cost-effective approach to produce biological products. Despite the continued interest in transient transfection, little is known regarding the transfection process at the intracellular level, particula
Autor:
Francesc Gòdia, Nicholas Simon Berrow, Laura Cervera, Sonia Gutiérrez-Granados, María Mercedes Segura
Publikováno v:
Biotechnology and Bioengineering. 112:934-946
Production of recombinant products in mammalian cell cultures can be achieved by stable gene expression (SGE) or transient gene expression (TGE). The former is based on the integration of a plasmid DNA into the host cell genome allowing continuous ge
Publikováno v:
Biotechnology and bioengineering. 114(11)
Transient transfection is a fast, flexible, and cost-effective approach to produce biological products. Despite the continued interest in transient transfection, little is known regarding the transfection process at the intracellular level, particula
Publikováno v:
Human Gene Therapy Methods; Vol 23
Canine adenovirus vectors (CAV2) are currently being evaluated for gene therapy, oncolytic virotherapy, and as vectors for recombinant vaccines. Despite the need for increasing volumes of purified CAV2 preparations for preclinical and clinical testin
Publikováno v:
Journal of Chromatography B. 846:124-131
Chromatography is deemed the most promising technology for large-scale purification of viral vectors. The authors have previously shown that heparin affinity chromatography could be successfully employed for the purification of VSV-G pseudotyped Molo
Publikováno v:
Applied microbiology and biotechnology. 100(9)
HIV-1 virus-like particles (VLPs) have great potential as new-generation vaccines. The novel CAP-T cell line is used for the first time to produce Gag-GFP HIV-1 VLPs by means of polyethylenimine (PEI)-mediated transient transfection. CAP-T cells are
Autor:
Sonia Gutiérrez-Granados, Laura Cervera, Francesc Gòdia, María Mercedes Segura, Javier Fuenmayor, Irene González-Domínguez
Publikováno v:
Applied microbiology and biotechnology. 99(23)
The manufacturing of biopharmaceuticals in mammalian cells typically relies on the use of stable producer cell lines. However, in recent years, transient gene expression has emerged as a suitable technology for rapid production of biopharmaceuticals.