An end-to-end pipeline based on open source deep learning tools for reliable analysis of complex 3D images of ovaries

Autor: Manon Lesage, Manon Thomas, Thierry Pécot, Tu-Ky Ly, Nathalie Hinfray, Remy Beaudouin, Michelle Neumann, Robin Lovell-Badge, Jérôme Bugeon, Violette Thermes

Publikováno v: Development (Cambridge, England)
Development (Cambridge, England), 2023, 150 (7), pp.dev201185. ⟨10.1242/dev.201185⟩

Computational analysis of bio-images by deep learning (DL) algorithms has made exceptional progress in recent years and has become much more accessible to non-specialists with the development of ready-to-use tools. The study of oogenesis mechanisms a

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a4fc1ef9484e7da918f1f295b7e0cbdc
https://hal.science/hal-04062159

C-ECi: a CUBIC-ECi combined clearing method for three-dimensional follicular content analysis in the fish ovary†

Autor: Manon Lesage, Manon Thomas, Jérôme Bugeon, Adèle Branthonne, Stéphanie Gay, Emilie Cardona, Marie Haghebaert, Fabrice Mahé, Julien Bobe, Violette Thermes

Publikováno v: Biology of Reproduction
Biology of Reproduction, Society for the Study of Reproduction, 2020, 103 (5), pp.1099-1109. ⟨10.1093/biolre/ioaa142⟩
Biology of Reproduction, 2020, 103 (5), pp.1099-1109. ⟨10.1093/biolre/ioaa142⟩

Deciphering mechanisms of oocyte development in the fish ovary still remain challenging, and a comprehensive overview of this process at the level of the organ is still needed. The recent development of optical tissue clearing methods has tremendousl

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::53463660621675901c1582f7c9c973cd
https://pubmed.ncbi.nlm.nih.gov/32776144

Inflammatory Type 2 cDCs Acquire Features of cDC1s and Macrophages to Orchestrate Immunity to Respiratory Virus Infection

Autor: Martin Guilliams, Sofie De Prijck, David L. Williams, Cedric Bosteels, Franca Ronchese, Katrijn Neyt, Bart N. Lambrecht, Yvan Saeys, Charlotte L. Scott, Manon Lesage, Hamida Hammad, Niels Vandamme, Liesbet Martens, Manon Vanheerswynghels, Mary J. van Helden, Victor Bosteels, Dorine Sichien, Els Louagie, Johannes U Mayer, Shiau-Choot Tang, Nincy Debeuf

Publikováno v: Immunity
Immunity, 52(6), 1039-1056. Cell Press
Cell Mol Immunol

Summary The phenotypic and functional dichotomy between IRF8+ type 1 and IRF4+ type 2 conventional dendritic cells (cDC1s and cDC2s, respectively) is well accepted; it is unknown how robust this dichotomy is under inflammatory conditions, when additi

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::62556a66607a657a973fc4a9b4cc7f96
https://doi.org/10.1016/j.immuni.2020.04.005