Rapid transcriptional pulsing dynamics of high expressing retroviral transgenes in embryonic stem cells.

Autor: Mandy Y M Lo, Sylvie Rival-Gervier, Peter Pasceri, James Ellis

Publikováno v: PLoS ONE, Vol 7, Iss 5, p e37130 (2012)

Single cell imaging studies suggest that transcription is not continuous and occurs as discrete pulses of gene activity. To study mechanisms by which retroviral transgenes can transcribe to high levels, we used the MS2 system to visualize transcripti

Externí odkaz: https://doaj.org/article/07d6c0e9951642f1b629841c4a0842bf

Kinetics and epigenetics of retroviral silencing in mouse embryonic stem cells defined by deletion of the D4Z4 element

Autor: Mandy Y. M. Lo, James Ellis, Matthew C. Lorincz, Peter Pasceri, Sylvie Rival-Gervier, Shahryar Khattak

Publikováno v: Molecular Therapy 8 (21), 1536-1550. (2013)
Molecular Therapy
Molecular Therapy, Cell Press, 2013, 21 (8), pp.1536-1550. ⟨10.1038/mt.2013.131⟩
Molecular Therapy, Nature Publishing Group, 2013, 21 (8), pp.1536-1550. ⟨10.1038/mt.2013.131⟩

Retroviral vectors are silenced in embryonic stem (ES) cells by epigenetic mechanisms whose kinetics are poorly understood. We show here that a 3'D4Z4 insulator directs retroviral expression with persistent but variable expression for up to 5 months.

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::93e4d6c775a04891541fb427afe522dd
https://pubmed.ncbi.nlm.nih.gov/23903574

Beta-globin LCR and intron elements cooperate and direct spatial reorganization for gene therapy

Autor: Eden Fussner, Angela Moffett, James Ellis, Mandy Y. M. Lo, Alla Buzina, David P. Bazett-Jones, Peter Pasceri, Rikki R. Bharadwaj, Akitsu Hotta, J. Victor Garcia-Martinez

Publikováno v: PLoS Genetics, Vol 4, Iss 4, p e1000051 (2008)
PLoS Genetics

The Locus Control Region (LCR) requires intronic elements within β-globin transgenes to direct high level expression at all ectopic integration sites. However, these essential intronic elements cannot be transmitted through retrovirus vectors and th

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e75477a6af3c45ce414ff21a9770bc56
http://europepmc.org/articles/PMC2271131?pdf=render