Zobrazeno 1 - 10
of 328
pro vyhledávání: '"Mandelate racemase"'
Autor:
Joshua A Hayden, Meghan C Hamilton, Lia Grandinetti, Nicole M. Easton, Oliver P Kuehm, Stephen L. Bearne, Colin D. Douglas, Martin St. Maurice
Publikováno v:
Biochemistry. 60:2508-2518
o-Carbonyl arylboronic acids such as 2-formylphenylboronic acid (2-FPBA) are employed in biocompatible conjugation reactions with the resulting iminoboronate adduct stabilized by an intramolecular N-B interaction. However, few studies have utilized t
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::54db55fadbdbb2fe56ba50d6da18bac2
https://doi.org/10.1021/acs.biochem.1c00374
https://doi.org/10.1021/acs.biochem.1c00374
Publikováno v:
Protein Engineering, Design and Selection. 32:261-270
Dihydroxy-acid dehydratase (DHAD) catalyzes the dehydration of R-2,3-dihydroxyisovalerate (DHIV) to 2-ketoisovalerate (KIV) using an Fe-S cluster as a cofactor, which is sensitive to oxidation and expensive to synthesize. In contrast, sugar acid dehy
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8917dd5f09eebbac6903afc7ed9358bb
https://doi.org/10.1093/protein/gzz042
https://doi.org/10.1093/protein/gzz042
Autor:
Christopher M. Fetter, Mitesh Nagar, Colin D. Douglas, Stephen L. Bearne, Zachary A. Morrison
Publikováno v:
Archives of Biochemistry and Biophysics. 666:116-126
Mandelate racemase (MR) catalyzes the interconversion of the enantiomers of mandelate using a two-base mechanism with Lys 166 acting as the Bronsted base to abstract the α-proton from (S)-mandelate. The resulting intermediate is subsequently re-prot
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::390c51138419f35d503ed091853eaeb0
https://doi.org/10.1016/j.abb.2019.03.011
https://doi.org/10.1016/j.abb.2019.03.011
Publikováno v:
Organic Process Research & Development. 22:1761-1771
Conventional enantioselective preparative chromatographic separation using columns packed with chiral stationary phase is characterized by a 50% yield constraint. Racemization of the undesired enan...
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::2e16dccd7c19aaf90106199ca8dfd45d
https://doi.org/10.1021/acs.oprd.8b00254
https://doi.org/10.1021/acs.oprd.8b00254
Publikováno v:
Protein Engineering, Design and Selection. 31:135-145
Mandelate racemase (MR) serves as a paradigm for our understanding of enzyme-catalyzed deprotonation of a carbon acid substrate. To facilitate structure-function studies on MR using non-natural amino acid substitutions, we engineered the Cys92Ser/Cys
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::dff5be29441be4bd2cffc422d23419e2
https://doi.org/10.1093/protein/gzy011
https://doi.org/10.1093/protein/gzy011
Akademický článek
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Akademický článek
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Publikováno v:
Biochemical and Biophysical Research Communications. 491:217-222
3,6-Anydro- l -galatonate cycloisomerase (ACI) catalyzes the cycloisomerization of a 3,6-anhydro- l -galactonic acid known as a novel metabolite in agarolytic bacteria. Here, we present 3-D structures of ACI from Vibrio sp. strain EJY3 ( Vej ACI) in
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::271c9bdc06e1c7bcdb8b125dc7cc5a42
https://doi.org/10.1016/j.bbrc.2017.07.080
https://doi.org/10.1016/j.bbrc.2017.07.080
Autor:
Stephen L. Bearne
Publikováno v:
Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics. 1865:619-630
Background Enzymes of the enolase superfamily (ENS) are mechanistically diverse, yet share a common partial reaction (abstraction of the α-proton from a carboxylate substrate). While the catalytic machinery responsible for the deprotonation reaction
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3b3dd7660d205f8b9e5e2c65dda22e9f
https://doi.org/10.1016/j.bbapap.2017.02.006
https://doi.org/10.1016/j.bbapap.2017.02.006
Autor:
Xiang Li, Lin-Feng Wang, Jian-He Xu, Yuan-Yuan Jia, Kan Yunchao, Lun-Guang Yao, Tang Cunduo, Hong-Ling Shi
Publikováno v:
Enzyme and microbial technology. 136
L-Phenylglycine (L-PHG) is a member of unnatural amino acids, and becoming more and more important as intermediate for pharmaceuticals, food additives and agrochemicals. However, the existing synthetic methods for L-PHG mainly rely on toxic cyanide c
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4e26d7903eac9211491b232aa87cd8c6
https://pubmed.ncbi.nlm.nih.gov/32331718
https://pubmed.ncbi.nlm.nih.gov/32331718