Výsledky vyhledávání - "Manda E. Gent"

Use of expressed sequence tag analysis and cDNA microarrays of the filamentous fungus Aspergillus nidulans

Autor: Rolf A. Prade, Stephen G. Oliver, Hugh H. Russell, Andrew H. Sims, Manda E. Gent, David C. Hoyle, Geoffrey Turner, Nigel Dunn-Coleman, Geoffrey D. Robson

Publikováno v: Fungal Genetics and Biology. 41:199-212

The use of microarrays in the analysis of gene expression is becoming widespread for many organisms, including yeast. However, although the genomes of a number of filamentous fungi have been fully or partially sequenced, microarray analysis is still

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::df0f5eb37ba8ea682517ea0f04aab7d3
https://doi.org/10.1016/j.fgb.2003.11.005

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Transcript analysis of 250 novel yeast genes from chromosome XIV

Autor: Mark Sefton, Dennis J. Verbart, Andrew Hayes, Yvon J. van Delft, Jose L. Cadahia, Rudi J. Planta, Stephen G. Oliver, Alistair J. P. Brown, Hélène Tournu, Martine De Jonge, Johan M. Thevelein, M. Esperanza Cerdán, L J Lombardía, Manda E. Gent, Carin P. A. M. Kolen, Joris Winderickx

Publikováno v: Yeast. 15:329-350

The European Functional Analysis Network (EUROFAN) is systematically analysing the function of novel Saccharomyces cerevisiae genes revealed by genome sequencing. As part of this effort our consortium has performed a detailed transcript analysis for

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::f147b7963c667d41e4f4981ab650d71a
https://doi.org/10.1002/(sici)1097-0061(19990315)15:4<329::aid-yea360>3.0.co

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XI. Yeast sequencing reports. Sequence analysis of a 3·5 kbEcoRI fragment from the left arm ofSaccharomyces cerevisiae chromosome XI reveals the location of theMBR1 gene and a sequence related to a GTPase-activating protein

Autor: Manda E. Gent, Stephen G. Oliver, C. M. James

Publikováno v: Yeast. 10:257-264

We present the DNA sequence analysis of a region covering a 3·5 kb EcoRI fragment from the left arm of chromosome XI from Saccharomyces cerevisiae. This region contains five open reading frames (ORFs) which code for proteins of greater than 100 amin

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::b19eb18495e5888d83a44aa4c7d16eab
https://doi.org/10.1002/yea.320100212

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XI. Yeast sequencing reports. Sequence analysis of a 10 kb fragment of yeast chromosome XI identifies theSMY1 locus and reveals sequences related to a pre-mRNA splicing factor and vacuolar ATPase subunit C plus a number of unidentified open reading frames

Autor: C. M. James, Stephen G. Oliver, Manda E. Gent, Keith J. Indge

Publikováno v: Yeast. 10:247-255

We report the DNA sequence analysis of a region on the left arm of chromosome XI of Saccharomyces cerevisiae extending over 10 kb. The region contains five open reading frames (ORFs) of greater than 100 amino acids which do not show significant overl

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::9c169e61dadde18d138009507cbf4cc3
https://doi.org/10.1002/yea.320100211

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Chemostats and Microarrays

Autor: Karin Lanthaler, Manda E. Gent

Publikováno v: Mycology ISBN: 9780849390807
The Aspergilli: Genomics, Medical Aspects, Biotechnology, and Research Methods

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::8ab929e8ef4ca321c7064370f1a62d2a
https://doi.org/10.1201/9781420008517.ch29

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The relative merits of the tetO2 and tetO7 promoter systems for the functional analysis of heterologous genes in yeast and a compilation of essential yeast genes with tetO2 promoter substitutions

Autor: Stephen G. Oliver, Kuangyu Yen, Paul Gitsham, Nianshu Zhang, Zorana Vujovic, Jill A. Wishart, J. Ross Miller, Manda E. Gent, Michael J. Osborn

Publikováno v: Yeast (Chichester, England). 23(4)

We have generated a collection of yeast strains, each of which has an essential yeast gene under the control of the tetracycline-responsive, tetO, promoter. Screens using first-generation promoter-swap strains uncovered the non-specific responsivenes

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::04bb0d08fd982347ef47d6e123b7675d
https://pubmed.ncbi.nlm.nih.gov/16544274

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Transcriptome analysis of recombinant protein secretion by Aspergillus nidulans and the unfolded-protein response in vivo

Autor: Stephen G. Oliver, Nigel Dunn-Coleman, Karin Lanthaler, Manda E. Gent, Andrew H. Sims, Geoffrey D. Robson

Publikováno v: Applied and environmental microbiology. 71(5)

Filamentous fungi have a high capacity for producing large amounts of secreted proteins, a property that has been exploited for commercial production of recombinant proteins. However, the secretory pathway, which is key to the production of extracell

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::19b61476f806a801cb75d2eb7093b22b
https://pubmed.ncbi.nlm.nih.gov/15870366

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Combining transcriptome data with genomic and cDNA sequence alignments to make confident functional assignments for Aspergillus nidulans genes

Autor: Nigel Dunn-Coleman, Stephen G. Oliver, Geoffrey D. Robson, Manda E. Gent, Andrew H. Sims

Publikováno v: Mycological research. 108(Pt 8)

Whole genome sequencing of several filamentous ascomycetes is complete or in progress; these species, such as Aspergillus nidulans, are relatives of Saccharomyces cerevisiae. However, their genomes are much larger and their gene structure more comple

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0373230939083b99c153dcc0c4dc638a
https://pubmed.ncbi.nlm.nih.gov/15449589

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Comparative genomic hybridization provides new insights into the molecular taxonomy of the Saccharomyces sensu stricto complex

Autor: Lubomira Stateva, Stephen G. Oliver, David C. Hoyle, Laura C. Edwards-Ingram, Andrew Hayes, Manda E. Gent

Publikováno v: Genome research. 14(6)

The science of taxonomy is constantly improving as new techniques are developed. Current practice is to construct phylogenetic trees based on the analysis of the DNA sequence of single genes, or parts of single genes. However, this approach has recen

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d478b3edda1e5de763631d9641cded93
https://pubmed.ncbi.nlm.nih.gov/15173111

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