7-Deazaguanines in DNA: functional and structural elucidation of a DNA modification system

Autor: Samanthi Herath Gedara, Evan Wood, Andrew Gustafson, Cui Liang, Shr-Hau Hung, Joshua Savage, Phuc Phan, Amit Luthra, Valérie de Crécy-Lagard, Peter Dedon, Manal A Swairjo, Dirk Iwata-Reuyl

Publikováno v: Nucleic Acids Research. 51:3836-3854

The modified nucleosides 2′-deoxy-7-cyano- and 2′-deoxy-7-amido-7-deazaguanosine (dPreQ0 and dADG, respectively) recently discovered in DNA are the products of the bacterial queuosine tRNA modification pathway and the dpd gene cluster, the latter

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::b3c63ff6c26c8a2fe375c08043ed2014
https://doi.org/10.1093/nar/gkad141

Specificity in the biosynthesis of the universal tRNA nucleoside N6-threonylcarbamoyl adenosine (t6A)—TsaD is the gatekeeper

Autor: Valérie de Crécy-Lagard, Manal A. Swairjo, Jo Marie Bacusmo, Christopher Deutsch, Dirk Iwata-Reuyl, William Swinehart, Amit Luthra, Paul F. Agris, Kathryn L. Sarachan

Publikováno v: RNA. 26:1094-1103

N6-threonylcarbamoyl adenosine (t6A) is a nucleoside modification found in all kingdoms of life at position 37 of tRNAs decoding ANN codons, which functions in part to restrict translation initiation to AUG and suppress frameshifting at tandem ANN co

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::bdd9f9fc7068e08bc47da6d991fd395a
https://doi.org/10.1261/rna.075747.120

Detection of preQ0 deazaguanine modifications in bacteriophage CAjan DNA using Nanopore sequencing reveals same hypermodification at two distinct DNA motifs

Autor: Sylvain Moineau, Liang Cui, Peter C. Dedon, Tue Kjærgaard Nielsen, Geoffrey Hutinet, Lars Hestbjerg Hansen, Manal A. Swairjo, Nikoline S. Olsen, Valérie de Crécy-Lagard, Alexander Byth Carstens, Witold Kot

Publikováno v: Kot, W, Olsen, N S, Nielsen, T K, Hutinet, G, de Crécy-Lagard, V, Cui, L, Dedon, P C, Carstens, A B, Moineau, S, Swairjo, M A & Hansen, L H 2020, ' Detection of preQ 0 deazaguanine modifications in bacteriophage CAjan DNA using Nanopore sequencing reveals same hypermodification at two distinct DNA motifs ', Nucleic Acids Research, vol. 48, no. 18, pp. 10383-10396 . https://doi.org/10.1093/nar/gkaa735
Kot, W, Olsen, N S, Nielsen, T K, Hutinet, G, de Crecy-Lagard, V, Cui, L, Dedon, P C, Carstens, A B, Moineau, S, Swairjo, M A & Hansen, L H 2020, ' Detection of preQ 0 deazaguanine modifications in bacteriophage CAjan DNA using Nanopore sequencing reveals same hypermodification at two distinct DNA motifs ', Nucleic Acids Symposium Series, vol. 48, no. 18, pp. 10383-10396 . https://doi.org/10.1093/nar/gkaa735
Nucleic Acids Research

In the constant evolutionary battle against mobile genetic elements (MGEs), bacteria have developed several defense mechanisms, some of which target the incoming, foreign nucleic acids e.g. restriction-modification (R-M) or CRISPR-Cas systems. Some o

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a0a2d52f1da8c6c7688c9e9fb9d5ab94
https://pure.au.dk/portal/da/publications/detection-of-preq0-deazaguanine-modifications-in-bacteriophage-cajan-dna-using-nanopore-sequencing-reveals-same-hypermodification-at-two-distinct-dna-motifs(312622ab-1976-4b3a-a198-2fa16fa64494).html

Fluorescent Fatty Acid Transfer from Bovine Serum Albumin to Phospholipid Vesicles: Collision or Diffusion Mediated Uptake

Autor: Bassam M Elmadhoun, Manal A Swairjo, Frank J Burczynski

Publikováno v: Journal of Pharmacy & Pharmaceutical Sciences, Vol 15, Iss 3 (2012)

Purpose: The extent of palmitate uptake by hepatocytes is dependent upon the surface charge of the extracellular binding protein. Specifically, hepatocyte uptake is greater when palmitate is bound to cationic binding proteins than when it is bound to

Externí odkaz: https://doaj.org/article/92fb3e77ce2f4ee9877c31e4d1bf373e

Discovery of novel bacterial queuine salvage enzymes and pathways in human pathogens

Autor: Daniel J. Payan, Sara Šepić, Steven C. Almo, Valérie de Crécy-Lagard, Peter C. Dedon, John A. Gerlt, Vinod Gadi, Manal A. Swairjo, Isabelle Martin-Verstraete, Yifeng Yuan, Seetharamsing Balamkundu, Ramesh Neelakandan, Chuan Fa Liu, Rémi Zallot, Tyler L. Grove

Publikováno v: Proceedings of the National Academy of Sciences of the United States of America
Proceedings of the National Academy of Sciences of the United States of America, National Academy of Sciences, 2019, 116 (38), pp.19126-19135. ⟨10.1073/pnas.1909604116⟩
Proceedings of the National Academy of Sciences of the United States of America, 2019, 116 (38), pp.19126-19135. ⟨10.1073/pnas.1909604116⟩

Queuosine (Q) is a complex tRNA modification widespread in eukaryotes and bacteria that contributes to the efficiency and accuracy of protein synthesis. Eukaryotes are not capable of Q synthesis and rely on salvage of the queuine base (q) as a Q prec

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0131c772c92189bcb23ca565b10e831e
https://cronfa.swan.ac.uk/Record/cronfa53171