Zobrazeno 1 - 10
of 141
pro vyhledávání: '"Manabu Ozawa"'
Multiple ageing effects on testicular/epididymal germ cells lead to decreased male fertility in mice
Autor:
Tsutomu Endo, Kiyonori Kobayashi, Takafumi Matsumura, Chihiro Emori, Manabu Ozawa, Shimpei Kawamoto, Daisuke Okuzaki, Keisuke Shimada, Haruhiko Miyata, Kentaro Shimada, Mayo Kodani, Yu Ishikawa-Yamauchi, Daisuke Motooka, Eiji Hara, Masahito Ikawa
Publikováno v:
Communications Biology, Vol 7, Iss 1, Pp 1-16 (2024)
Abstract In mammals, females undergo reproductive cessation with age, whereas male fertility gradually declines but persists almost throughout life. However, the detailed effects of ageing on germ cells during and after spermatogenesis, in the testis
Externí odkaz:
https://doaj.org/article/623ca58762e34a14a21188196ebbee61
Autor:
Takahiro Eguchi, Tohru Tezuka, Yuji Watanabe, Akane Inoue-Yamauchi, Hiroshi Sagara, Manabu Ozawa, Yuji Yamanashi
Publikováno v:
iScience, Vol 27, Iss 2, Pp 108997- (2024)
Summary: The neuromuscular junction (NMJ) forms centrally in myotubes and, as the only synapse between motor neuron and myotube, are indispensable for motor activity. The midmuscle formation of NMJs, including midmuscle-restricted expression of NMJ-r
Externí odkaz:
https://doaj.org/article/7e4575ae1e2b4903b70574cd4d6d1a6a
Autor:
Manabu Ozawa, Hideto Mori, Tsutomu Endo, Yu Ishikawa-Yamauchi, Daisuke Motooka, Chihiro Emori, Masahiro Ikawa
Publikováno v:
iScience, Vol 26, Iss 12, Pp 108456- (2023)
Summary: Male fertility decreases with aging, with spermatogenic decline being one of its causes. Altered testis environment is suggested as a cause of the phenotype; however, the associated mechanisms remain unclear. Herein, we investigated the age-
Externí odkaz:
https://doaj.org/article/fb2a7fd3eb684b9eb58f659567a5a555
Autor:
Manabu Ozawa, Jumpei Taguchi, Kento Katsuma, Yu Ishikawa-Yamauchi, Mio Kikuchi, Reiko Sakamoto, Yasuhiro Yamada, Masahito Ikawa
Publikováno v:
Scientific Reports, Vol 12, Iss 1, Pp 1-13 (2022)
Abstract Gene targeting of embryonic stem (ES) cells followed by chimera production has been conventionally used for developing gene-manipulated mice. Although direct knock-in (KI) using murine zygote via CRISPR/Cas9-mediated genome editing has been
Externí odkaz:
https://doaj.org/article/e53033ea683742beaa843bd39da7ec56
Autor:
Jumpei Taguchi, Hirofumi Shibata, Mio Kabata, Masaki Kato, Kei Fukuda, Akito Tanaka, Sho Ohta, Tomoyo Ukai, Kanae Mitsunaga, Yosuke Yamada, So I Nagaoka, Sho Yamazawa, Kotaro Ohnishi, Knut Woltjen, Tetsuo Ushiku, Manabu Ozawa, Mitinori Saitou, Yoichi Shinkai, Takuya Yamamoto, Yasuhiro Yamada
Publikováno v:
Nature Communications, Vol 12, Iss 1, Pp 1-18 (2021)
The mechanisms by which in vivo expression of the Yamanaka transcription factors (OSKM) renders somatic cells permissive for differentiation remain unclear. Here, the authors show that in vivo reprogramming using OSKM generates germ cell tumors and d
Externí odkaz:
https://doaj.org/article/ce67fa07507345ab98bca02b31126aed
Autor:
Thanh Hoang, Dong Won Kim, Haley Appel, Nicole A. Pannullo, Patrick Leavey, Manabu Ozawa, Sika Zheng, Minzhong Yu, Neal S. Peachey, Seth Blackshaw
Publikováno v:
Cell Reports, Vol 39, Iss 11, Pp 110849- (2022)
Summary: Direct reprogramming of glia into neurons is a potentially promising approach for the replacement of neurons lost to injury or neurodegenerative disorders. Knockdown of the polypyrimidine tract-binding protein Ptbp1 has been recently reporte
Externí odkaz:
https://doaj.org/article/d7a39380ef884540887e51512ff4db40
Autor:
Manami SENOO, Hiroshi HOZOJI, Yu ISHIKAWA-YAMAUCHI, Takashi TAKIJIRI, Sho OHTA, Tomoyo UKAI, Mio KABATA, Takuya YAMAMOTO, Yasuhiro YAMADA, Masahito IKAWA, Manabu OZAWA
Publikováno v:
The Journal of Reproduction and Development, Vol 66, Iss 5, Pp 459-467 (2020)
PTBP1, a well-conserved RNA-binding protein, regulates cellular development by tuning posttranscriptional mRNA modification such as alternative splicing (AS) or mRNA stabilization. We previously revealed that the loss of Ptbp1 in spermatogonia causes
Externí odkaz:
https://doaj.org/article/b663433465594b679f115f4d9a3ebd35
Autor:
Shingo Komura, Kenji Ito, Sho Ohta, Tomoyo Ukai, Mio Kabata, Fumiaki Itakura, Katsunori Semi, Yutaka Matsuda, Kyoichi Hashimoto, Hirofumi Shibata, Masamitsu Sone, Norihide Jo, Kazuya Sekiguchi, Takatoshi Ohno, Haruhiko Akiyama, Katsuji Shimizu, Knut Woltjen, Manabu Ozawa, Junya Toguchida, Takuya Yamamoto, Yasuhiro Yamada
Publikováno v:
Nature Communications, Vol 10, Iss 1, Pp 1-19 (2019)
The EWS-ATF1 fusion gene causes clear cell sarcoma (CCS). Here, the authors show that the downstream effects of EWS-ATF1 expression are strictly context dependent, and reveal the cell of origin for CCS to be Tppp3-expressing cells in peripheral nerve
Externí odkaz:
https://doaj.org/article/d6a0a59accc9488fa4d118ffc7d1f1f9
Autor:
Tadashi Yamamuro, Shuhei Nakamura, Yu Yamano, Tsutomu Endo, Kyosuke Yanagawa, Ayaka Tokumura, Takafumi Matsumura, Kiyonori Kobayashi, Hideto Mori, Yusuke Enokidani, Gota Yoshida, Hitomi Imoto, Tsuyoshi Kawabata, Maho Hamasaki, Akiko Kuma, Sohei Kuribayashi, Kentaro Takezawa, Yuki Okada, Manabu Ozawa, Shinichiro Fukuhara, Takashi Shinohara, Masahito Ikawa, Tamotsu Yoshimori
Publikováno v:
PLoS Genetics, Vol 17, Iss 8 (2021)
Autophagy degrades unnecessary proteins or damaged organelles to maintain cellular function. Therefore, autophagy has a preventive role against various diseases including hepatic disorders, neurodegenerative diseases, and cancer. Although autophagy i
Externí odkaz:
https://doaj.org/article/d2de1c9609cc4eabada556ef6ddc88f3
Publikováno v:
The Journal of Reproduction and Development, Vol 65, Iss 1, Pp 37-46 (2018)
Polypyrimidine tract-binding protein 1 (PTBP1) is a highly conserved RNA-binding protein that is a well-known regulator of alternative splicing. Testicular tissue is one of the richest tissues with respect to the number of alternative splicing mRNA i
Externí odkaz:
https://doaj.org/article/e34ff74e06064549bcc47e2d1a3e398e