Zobrazeno 1 - 10
of 13
pro vyhledávání: '"Mamiko Masutani"'
Publikováno v:
FEBS Letters. 594:3020-3031
Testis-expressed gene 101 (TEX101) is a glycosyl-phosphatidylinositol-anchored glycoprotein essential for sperm fertility and spermatogenesis. TEX101 interacts with lymphocyte antigen 6 complex, locus K (Ly6k) as well as a disintegrin and metallopept
Autor:
Mamiko Masutani, Hiroaki Imataka, Tominari Kobayashi, Satoshi Mikami, Kurumi Mishima, Kodai Machida
Publikováno v:
The Journal of Biological Chemistry
Background: The mechanism by which the encephalomyocarditis virus protein 2A-2B is processed into 2A and 2B is unknown. Results: A translation system reconstituted with defined human factors recapitulates 2A-2B processing. Conclusion: 2A-2B processin
Publikováno v:
Protein Expression and Purification. 87:5-10
Many biologically important factors are composed of multiple subunits. To study the structure and function of the protein complexes and the role of each subunit, a rapid and efficient method to prepare recombinant protein complexes is needed. In this
Autor:
Yuri Nishino, Mamiko Masutani, Tominari Kobayashi, Atsuo Miyazawa, Satoshi Mikami, Kodai Machida, Hiroaki Imataka
Publikováno v:
Protein Expression and Purification. 82:61-69
The eukaryotic cytosolic chaperonin CCT (chaperonin-containing TCP-1) assists folding of newly synthesized polypeptides. The fully functional CCT is built from two identical rings, each composed of single copies of eight distinct subunits. To study t
Autor:
Mamiko Masutani, Hiroaki Imataka, Kodai Machida, Satoshi Mikami, Yusuke Nakamura, Tominari Kobayashi
Publikováno v:
Biotechnology Letters. 34:67-73
Virus particles are used in vaccination, drug delivery, and material sciences. Here we devised a system where the RNA virus encephalomyocarditis virus (EMCV) is synthesized from DNA templates in vitro. When a plasmid or a PCR product harboring the fu
Publikováno v:
The EMBO Journal. 26:3373-3383
Eukaryotic translation initiation factor (eIF)3 is the largest eIF ( approximately 650 kDa), consisting of 10-13 different polypeptide subunits in mammalian cells. To understand the role of each subunit, we successfully reconstituted a human eIF3 com
Autor:
Tominari Kobayashi, Kodai Machida, Kosaku Ueda, Jun Yukigai, Yuri Nishino, Atsuo Miyazawa, Mamiko Masutani, Hiroaki Imataka
Publikováno v:
Biotechnology letters. 35(3)
Virus particles are promising vehicles and templates for vaccination, drug delivery and material sciences. Although infectious picornaviruses can be synthesized from genomic or synthetic RNA by cell-free protein expression systems derived from mammal
Publikováno v:
Journal of biochemistry. 150(4)
To study the relationship between translation and replication of encephalomyocarditisvirus (EMCV) RNA, we established a cell-free RNA replication system by employing a human cell extracts-based in vitro translation system. In this system, a cis-EMCV
Autor:
Tominari Kobayashi, Mamiko Masutani, Kodai Machida, Shigeyuki Yokoyama, Satoshi Mikami, Hiroaki Imataka
Publikováno v:
Biotechnology letters. 32(7)
Human cell-derived in vitro protein synthesis systems are useful for the production of recombinant proteins. Productivity can be increased by supplementation with GADD34, a protein that is difficult to express in and purify from E. coli. Deletion of
Publikováno v:
Protein expression and purification. 62(2)
The aim of this study was to develop an efficient cell-free protein expression system derived from mammalian cells. We established a HeLa cell-based in vitro coupled transcription/translation system with T7 RNA polymerase and a plasmid that harbored