Zobrazeno 1 - 10
of 65
pro vyhledávání: '"Malihe Keramati"'
Publikováno v:
AMB Express, Vol 14, Iss 1, Pp 1-14 (2024)
Abstract In the present study, low- and high-molecular-weight hyaluronic acids (LMW-HA and HMW-HA) were synthesized in vitro by truncated Streptococcus equisimilis hyaluronan synthases (SeHAS). The enzyme kinetic parameters were determined for each e
Externí odkaz:
https://doaj.org/article/2988cac57fbd499cb7e52f572763b352
Autor:
Shadi Afrasiabi, Fatemeh Sadat Amjad Zanjani, Gholamreza Ahmadian, Reza Ahangari Cohan, Malihe Keramati
Publikováno v:
AMB Express, Vol 13, Iss 1, Pp 1-12 (2023)
Abstract Hyaluronic acid (HA), composed of glucuronic acid (GlcUA) and N-acetyl glucoseamine (GlcNAc), is a versatile biopolymer with high commercial value and innumerous physiological roles and pharmaceutical applications. The hasA gene has main rol
Externí odkaz:
https://doaj.org/article/5aec9ddb8df84ba58f9ac01d19f01028
Publikováno v:
AMB Express, Vol 12, Iss 1, Pp 1-8 (2022)
Abstract Design of experiment (DOE) is a statistical approach for designing, performing, and interpreting a large set of data with the minimum number of tests. In our previous study, we developed a novel Hsp27 SILEX system for production of recombina
Externí odkaz:
https://doaj.org/article/566310b70fcc4b8a9b375c4b15207f10
Autor:
Fatemeh Sadat Amjad Zanjani, Shadi Afrasiabi, Dariush Norouzian, Gholamreza Ahmadian, Sara Ali Hosseinzadeh, Alireza Fayazi Barjin, Reza Ahangari Cohan, Malihe Keramati
Publikováno v:
AMB Express, Vol 12, Iss 1, Pp 1-9 (2022)
Key points The strains containing truncated forms of SeHAS enzysme are able to produce HA. The HA from all recombinant strains was the same and low Mw. Deletion of C-terminal region of SeHAS was not effective on Mw.
Externí odkaz:
https://doaj.org/article/bc44b656b0424a8ba22bf9c8e0b00551
Autor:
Fatemeh Sadat Shariati, Dariush Norouzian, Vahideh Valizadeh, Reza Ahangari Cohan, Malihe Keramati
Publikováno v:
Microbial Cell Factories, Vol 20, Iss 1, Pp 1-11 (2021)
Abstract Background Identification of high-expressing colonies is one of the main concerns in the upstream process of recombinant protein development. The common method to screen high-producing colonies is SDS-PAGE, a laborious and time-consuming pro
Externí odkaz:
https://doaj.org/article/2a792a48bea24548ba84e0e6dabac5d9
Autor:
Fatemeh Sadat Shariati, Malihe Keramati, Vahideh Valizadeh, Reza Ahangari Cohan, Dariush Norouzian
Publikováno v:
Scientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
Abstract IPTG-inducible promoter is popularly used for the expression of recombinant proteins. However, it is not suitable at the industrial scale due to the high cost and toxicity on the producing cells. Recently, a Self-Inducible Expression (SILEX)
Externí odkaz:
https://doaj.org/article/e299f1fcf6914aabbb490ac40c59350e
Autor:
Masoud Seyedinkhorasani, Reza Ahangari Cohan, Saeid Taghavi Fardood, Farzin Roohvand, Dariush Norouzian, Malihe Keramati
Publikováno v:
Iranian Biomedical Journal, Vol 24, Iss 3, Pp 192-200 (2020)
Background: Protein purification is the most complicated issue in the downstream processes of recombinant protein production; therefore, improved selective purification methods are important. Affinity-based protein purification method using polyhisti
Externí odkaz:
https://doaj.org/article/b485ed0533034b04894c3469e61f11c4
Publikováno v:
Iranian Biomedical Journal, Vol 24, Iss 1, Pp 15-23 (2020)
Background: Streptokinase (SK), a heterogeneous plasminogen activator (PA) protein from groups A, C, and G streptococci (GAS, GCS, GGS, respectively) contains three structural domains (SKα, SKβ, and SKg). Based on the variable region of SKβ, GAS-S
Externí odkaz:
https://doaj.org/article/fac0cc35a6e948f08422e80e6368fa73
Publikováno v:
Iranian Biomedical Journal, Vol 24, Iss 1, Pp 15-23 (2020)
Background: SK, a heterogeneous PA protein from groups A, C, and G streptococci (GAS, GCS, GGS, respectively) contains three structural domains (SKα, SKβ, and SK). Based on the variable region of SKβ, GAS-SKs (ska) are clustered as SK1 and SK2
Externí odkaz:
https://doaj.org/article/e54d5b9d7dcb456aa2e4db13ff1e4583
Publikováno v:
Trends in Peptide and Protein Sciences, Vol 4, Pp 1-6 (e10) (2019)
The EpCAM (epithelial cell adhesion molecule) is a cell surface antigen over expressed in many types of epithelial cell cancers including colon, stomach, pancreas, lung, ovarian, and breast. So, it can be an attractive target for active and passive i
Externí odkaz:
https://doaj.org/article/dabdfb199c054c259566847bbc81ca3f