Zobrazeno 1 - 10
of 13
pro vyhledávání: '"Malgorzata M. Slupska"'
Autor:
Angela G. King, Sorel Fitz-Gibbon, Malgorzata M. Slupska, John Besemer, Jeffrey H Miller, Mark Borodovsky
Publikováno v:
Journal of Molecular Biology. 309:347-360
We mapped transcription start sites for ten unrelated protein-encoding Pyrobaculum aerophilum genes by primer extension and S 1 nuclease mapping. All of the mapped transcripts start at the computationally predicted translation start codons, two of wh
Autor:
Ju-Huei Chiang, Wendy M. Clendenin, Bruce Demple, Donny Wong, Malgorzata M. Slupska, Jeffrey H Miller, Hanjing Yang
Publikováno v:
Nucleic Acids Research. 29:743-752
Adenine-DNA glycosylase MutY of Escherichia coli catalyzes the cleavage of adenine when mismatched with 7,8-dihydro-8-oxoguanine (GO), an oxidatively damaged base. The biological outcome is the prevention of C/G→A/T transversions. The molecular mec
Autor:
Alexis Conrad, Malgorzata M. Slupska, Ju-Huei Chiang, Jean Lee Stewart, Lisa Amii, Jeffrey H Miller, Wendy M. Luther
Publikováno v:
Genes to Cells. 5:425-437
Background Not all of the enzymatic pathways involved in genetic rearrangements have been elucidated. While some rearrangements occur by recombination at areas of high homology, others are mediated by short, often interrupted homologies. We have prev
Autor:
Malgorzata M. Slupska, Annie Yeung, Jeffrey H Miller, Jean Lee Stewart, Pauline Funchain, Rose Lin
Publikováno v:
Genetics. 154:959-970
We have examined the composition of members of mutator populations of Escherichia coli by employing an extensive set of phenotypic screens that allow us to monitor the function of >700 genes, constituting ~15% of the genome. We looked at mismatch rep
Autor:
Hanjing Yang, Ying-Fei Wei, Alexis Conrad, Jeffrey H Miller, Malgorzata M. Slupska, Ju-Huei Chiang, Wendy M. Luther, Yu-Rong Xia, Sorel Fitz-Gibbon, Isabella T. Phan, Diana M. Shih, Claudia Baikalov, Jennifer H. Tai
Publikováno v:
Journal of Biological Chemistry. 275:8844-8853
Proteins containing the Nudix box "GX(5)EX(7)REUXEEXGU" (where U is usually Leu, Val, or Ile) are Nudix hydrolases, which catalyze the hydrolysis of a variety of nucleoside diphosphate derivatives. Here we report cloning and characterization of a hum
Publikováno v:
Journal of Bacteriology. 181:6210-6213
We have previously described the hMYH cDNA and genomic clones (M. M. Slupska et al., J. Bacteriol. 178:3885–3892, 1996). Here, we report that the enzyme expressed from an hMYH cDNA clone in Escherichia coli complements the mutator phenotype in a mu
Autor:
Rose Lin, Angela G. King, Jeffrey H Miller, Claudia Baikalov, Louise I. Lu, Chantal Lackey, Ju-Huei Chiang, Emily F. Mao, Malgorzata M. Slupska
Publikováno v:
Journal of Bacteriology. 180:5712-5717
We previously described Escherichia coli mutator tRNAs that insert glycine in place of aspartic acid and postulated that the elevated mutation rate results from generating a mutator polymerase. We suggested that the proofreading subunit of polymerase
Publikováno v:
Gene. 336(1)
An effective DNA replacement system has been established for engineering large fragment insertions into the chromosome of Escherichia coli. The DNA replacement plasmid, pHybrid I, was first constructed based on the bacterial artificial chromosome (BA
Autor:
E. Mao, Jean Lee Stewart, Jeffrey H Miller, Malgorzata M. Slupska, Pauline Funchain, Wendy M. Clendenin, A. Yeung
Publikováno v:
Cold Spring Harbor symposia on quantitative biology. 65
Autor:
Ju-Huei Chiang, Jeffrey H Miller, Lilit Garibyan, Erika Wolff, Malgorzata M. Slupska, Annie Yeung, Hanjing Yang, Anh Nguyen, Wendy M. Clendenin, Tiffany T. Huang, Pauline Funchain
Publikováno v:
Genetics. 162(1)
Nucleoside diphosphate (NDP) kinase is one of the enzymes that maintains triphosphate pools. Escherichia coli strains (ndk) lacking this enzyme have been shown to be modest base substitution mutators, and two members of the human family of NDP kinase