Zobrazeno 1 - 10
of 13
pro vyhledávání: '"Makoto Iwane"'
Publikováno v:
Neuroscience Letters. 260:5-8
TAK-147, a potent acetylcholinesterase (AChE) inhibitor, potentiated choline acetyltransferase (ChAT) activity in cultured rat septal cholinergic neurons in a concentration-dependent manner with an EC50 value of 4.47 nM. Donepezil, another potent ACh
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ed56318e3bfc6a0dc22e8fce29cbe4ed
https://doi.org/10.1016/s0304-3940(98)00943-4
https://doi.org/10.1016/s0304-3940(98)00943-4
Autor:
Makoto Iwane, Koichi Igarashi, Takuya Watanabe, S. Matsumoto, Reiko Sasada, Asae Shintani, Yoshihiko Kaisho
Publikováno v:
Applied Microbiology and Biotechnology. 41:225-232
In order to obtain high-level expression of recombinant human neurotrophin-3 (NT-3), we constructed several types of expression plasmids and examined several cell lines for expression of the human NT-3 gene. The highest level production of the recomb
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f33fb4d35e95e708e3e39c9c9f16b075
https://doi.org/10.1007/bf00186964
https://doi.org/10.1007/bf00186964
Autor:
Atsushi Kakinuma, Koji Yoshimura, Shizue Nakagawa, Yoshihiko Kaisho, Kenji Kawahara, Yumiko Kitamura, Reiko Sasada, Asae Shintani, Makoto Iwane, Kazuo Nakahama
Publikováno v:
Biochemical and Biophysical Research Communications. 171:116-122
The human NGF gene was isolated and inserted downstream from murine leukemia virus LTR in a plasmid having dihydrofolate reductase cDNA. The expression plasmid was introduced into CHO cells. Selection of the transformants for the resistance to methot
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d026f637d0c088b3b6ae6039ba214cf3
https://doi.org/10.1016/0006-291x(90)91364-x
https://doi.org/10.1016/0006-291x(90)91364-x
Publikováno v:
Biochemical and biophysical research communications. 196(3)
We have generated and characterized a monoclonal antibody to human nerve growth factor (hNGF). The monoclonal antibody NGFA-133 neutralizes hNGF activity, as assayed by neurite-outgrowth of nerve cells from chick embryonal dorsal root ganglion. Using
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::681c2b1df3177b7e72820d144812218f
https://pubmed.ncbi.nlm.nih.gov/8250905
https://pubmed.ncbi.nlm.nih.gov/8250905
Autor:
Akio Shino, Kenji Takami, Yoshihiro Kiyota, Koichi Igarashi, Masaya Tohyama, Akio Wanaka, Makoto Iwane, Masaomi Miyamoto, Ryoichi Tsukuda, Sadao Shiosaka
Publikováno v:
Experimental brain research. 97(2)
Recently, we demonstrated that transient forebrain ischemia in rats leads to an early and strong induction of basic fibroblast growth factor (bFGF) synthesis in astrocytes in the injured brain regions. In this study, in order to clarify the targets o
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8f4c7a7a82a089d95ec0c3b090af93d9
https://pubmed.ncbi.nlm.nih.gov/8150041
https://pubmed.ncbi.nlm.nih.gov/8150041
Autor:
Makoto Iwane, Yoshihiro Kiyota, Masaomi Miyamoto, Kenji Takami, Ryoichi Tsukuda, Sadao Shiosaka
Publikováno v:
Experimental brain research. 90(1)
We examined the time course of basic fibroblast growth factor (bFGF) immunoreactivity and its mRNA level mainly in the hippocampus after transient forebrain ischemia using immunohistochemistry, enzyme immunoassay (EIA), Western blot analysis and in s
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::346fdf76de22ae9bd0cccf59154cc0a0
https://pubmed.ncbi.nlm.nih.gov/1521598
https://pubmed.ncbi.nlm.nih.gov/1521598
Autor:
Kenji Takami, Yoshihiro Kiyota, Ryoichi Tsukuda, Akio Shino, Makoto Iwane, Masaomi Miyamoto, Akinobu Nagaoka
Publikováno v:
Brain research. 545(1-2)
Using immunohistochemical techniques, a study was conducted to determine whether basic fibroblast growth factor (bFGF) is generated as one of the ‘self-repair’ responses in rat brain following transient forebrain ischemia. In normal brain, slight
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a14c7f19918fc163350c83bb6cb744f0
https://pubmed.ncbi.nlm.nih.gov/1860053
https://pubmed.ncbi.nlm.nih.gov/1860053
Publikováno v:
岐阜藥科大學紀要 = The annual proceedings of Gifu College of Pharmacy. 41:89
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=jairo_______::aa6548f61513d0f6992b16c266e6a51e
http://id.nii.ac.jp/1076/00009205/
http://id.nii.ac.jp/1076/00009205/
Autor:
Koichi Igarashi, Shizue Nakagawa, Masaharu Seno, Reiko Sasada, Tsutomu Kurokawa, Makoto Iwane
Publikováno v:
Biochemical and Biophysical Research Communications. 146:470-477
The cDNA encoding human basic fibroblast growth factor was expressed in E. coli under the control of trp promoter. Bacterially synthesized hbFGF was highly purified using a heparin affinity HPLC column. By this chromatography, hbFGF was eluted as fou
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::784a2b6079ae9fd699cfc4abc9cf1ddb
https://doi.org/10.1016/0006-291x(87)90553-5
https://doi.org/10.1016/0006-291x(87)90553-5
Publikováno v:
Molecular and Cellular Biology. 8:588-594
The expression of human basic fibroblast growth factor (bFGF) cDNA in mouse BALB/c 3T3 clone A31 cells induced morphological transformation. These transformed cells grew well and reached more than a sixfold-higher saturation density than parental A31
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::12f0964c8e9df7c2445e99405e8e017d
https://doi.org/10.1128/mcb.8.2.588
https://doi.org/10.1128/mcb.8.2.588