Zobrazeno 1 - 10
of 141
pro vyhledávání: '"Makio Tokunaga"'
Publikováno v:
SoftwareX, Vol 23, Iss , Pp 101462- (2023)
Single-molecule imaging is a promising method for direct quantification of the dynamics and distribution of biomolecules in living cells. Although numerous methods have been developed to gain biological insights into molecular behavior, the high dive
Externí odkaz:
https://doaj.org/article/a5008f8175934ae19cc12f881bfc7dd5
Publikováno v:
Scientific Reports, Vol 7, Iss 1, Pp 1-14 (2017)
Abstract The dynamic properties of molecules in living cells are attracting increasing interest. We propose a new method, moving subtrajectory analysis using single-molecule tracking, and demonstrate its utility in the spatiotemporal quantification o
Externí odkaz:
https://doaj.org/article/34e1478115ed46ee9c70b10cff6b814d
Autor:
Ana V Miletic, Daniel B Graham, Kumiko Sakata-Sogawa, Michio Hiroshima, Michael J Hamann, Saso Cemerski, Tracie Kloeppel, Daniel D Billadeau, Osami Kanagawa, Makio Tokunaga, Wojciech Swat
Publikováno v:
PLoS ONE, Vol 4, Iss 8, p e6599 (2009)
BACKGROUND:T cell receptor (TCR) engagement leads to formation of signaling microclusters and induction of rapid and dynamic changes in the actin cytoskeleton, although the exact mechanism by which the TCR initiates actin polymerization is incomplete
Externí odkaz:
https://doaj.org/article/531bd18f9d744b3db2490e47f9786ecb
Autor:
Ning Zhao, Yotaro Yamamoto, Yang Liu, Hiroshi Kimura, Timothy J. Stasevich, Yuma Ito, Masato T. Kanemaki, Yoshifusa Sadamura, Makio Tokunaga
Publikováno v:
Genes to Cells. 26:905-926
In eukaryotic nuclei, chromatin loops mediated through cohesin are critical structures that regulate gene expression and DNA replication. Here we demonstrate a new method to visualize endogenous genomic loci using synthetic zinc-finger proteins harbo
Autor:
Yuma Ito, Makio Tokunaga
Publikováno v:
Biophysical Journal. 122:429a
Autor:
Yuko Sato, Makio Tokunaga, Tetsuya Handa, Satoshi Uchino, Yasuyuki Ohkawa, Yuma Ito, Hiroshi Kimura
Publikováno v:
The Journal of Cell Biology
Uchino et al. have developed a genetically encoded probe to specifically detect the Ser2-phosphorylated, elongating form of RNA polymerase II in living cells. Ser2-phosphorylated RNA polymerase foci exhibit more dynamic motion than chromatin replicat
Autor:
Yasuyuki Ohkawa, Tetsuya Handa, Hiroshi Kimura, Satoshi Uchino, Yuma Ito, Makio Tokunaga, Yuko Sato
In eukaryotic nuclei, most genes are transcribed by RNA polymerase II (RNAP2). How RNAP2 transcription is regulated in the nucleus is a key to understanding the genome and cell function. The largest subunit of RNAP2 has a long heptapeptide repeat (Ty
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::410f9d53367af2d5c6c5d146b1c146c7
https://doi.org/10.1101/2021.04.27.441582
https://doi.org/10.1101/2021.04.27.441582
Autor:
Haruka Matsumori, Kenji Watanabe, Hiroaki Tachiwana, Tomoko Fujita, Yuma Ito, Makio Tokunaga, Kumiko Sakata-Sogawa, Hiroko Osakada, Tokuko Haraguchi, Akinori Awazu, Hiroshi Ochiai, Yuka Sakata, Koji Ochiai, Tsutomu Toki, Etsuro Ito, Ilya G Goldberg, Kazuaki Tokunaga, Mitsuyoshi Nakao, Noriko Saitoh
Publikováno v:
Life science alliance. 5(7)
The nucleolus is the site of ribosome assembly and formed through liquid–liquid phase separation. Multiple ribosomal DNA (rDNA) arrays are bundled in the nucleolus, but the underlying mechanism and significance are unknown. In the present study, we
Autor:
Yuma Ito, Makio Tokunaga
Publikováno v:
Biophysical Journal. 121:364a
Autor:
Haruka Matsumori, Kenji Watanabe, Hiroaki Tachiwana, Tomoko Fujita, Yuma Ito, Makio Tokunaga, Kumiko Sakata-Sogawa, Hiroko Osakada, Tokuko Haraguchi, Akinori Awazu, Hiroshi Ochiai, Yuka Sakata, Koji Ochiai, Tsutomu Toki, Etsuro Ito, Goldberg, Ilya G., Kazuaki Tokunaga, Mitsuyoshi Nakao, Noriko Saitoh
Publikováno v:
Life Science Alliance; Jul2022, Vol. 5 Issue 7, p1-18, 18p