Zobrazeno 1 - 10
of 107
pro vyhledávání: '"Maki HIRATA"'
Autor:
Megumi Nagahara, Zhao Namula, Qingyi Lin, Koki Takebayashi, Nanaka Torigoe, Bin Liu, Fuminori Tanihara, Takeshige Otoi, Maki Hirata
Publikováno v:
Veterinary World, Vol 17, Iss 8, Pp 1748-1752 (2024)
Background and Aim: The antioxidant effects of ergothioneine (EGT) on in vitro culture of porcine zygote are not well established. The study investigated the impact of EGT supplementation on meiotic competence and development of porcine oocytes. Mate
Externí odkaz:
https://doaj.org/article/a1f1324a33744182965765bdeb004465
Autor:
Quynh Anh Le, Manita Wittayarat, Zhao Namula, Qingyi Lin, Koki Takebayashi, Maki Hirata, Fuminori Tanihara, Lanh Thi Kim Do, Takeshige Otoi
Publikováno v:
Veterinary World, Vol 15, Iss 9, Pp 2210-2216 (2022)
Background and Aim: Mosaicism – the presence of both wild-type and mutant alleles – is a serious problem for zygotic gene modification through gene editing using the Clustered regularly interspaced short palindromic repeats-Cas9 (CRISPR/ Cas9) sy
Externí odkaz:
https://doaj.org/article/48c4cb56bb1744bda267d216dd650be8
Autor:
Zhao Namula, Quynh Anh Le, Manita Wittayarat, Qingyi Lin, Koki Takebayashi, Maki Hirata, Lanh Thi Kim Do, Fuminori Tanihara, Takeshige Otoi
Publikováno v:
Veterinary World, Vol 15, Iss 2, Pp 496-501 (2022)
Background and Aim: We previously developed the gene-editing by electroporation (EP) of Cas9 protein method, in which the CRISPR/Cas9 system was introduced into porcine in vitro fertilized (IVF) zygotes through EP to disrupt a target gene. This metho
Externí odkaz:
https://doaj.org/article/72515af73328458384d77c54cc826003
Autor:
Zhao Namula, Maki Hirata, Quynh Anh Le, Qingyi Lin, Koki Takebayashi, Naoaki Yoshimura, Fuminori Tanihara, Chommanart Thongkittidilok, Takeshige Otoi
Publikováno v:
Veterinary Medicine and Science, Vol 8, Iss 1, Pp 164-169 (2022)
Abstract Background Increasing the permeability of the zona pellucida (ZP) of oocytes before CRISPR/Cas9 electroporation may improve the efficiency of gene editing; however, the effects of this approach on subsequent developmental processes are uncle
Externí odkaz:
https://doaj.org/article/09413eb19a4b49469c97a4af02007d69
Autor:
Chommanart Thongkittidilok, Maki Hirata, Qingyi Lin, Nanaka Torigoe, Bin Liu, Yoko Sato, Megumi Nagahara, Fuminori Tanihara, Takeshige Otoi
Publikováno v:
Veterinary Medicine International, Vol 2023 (2023)
Pigs rarely develop cancer; however, tumour protein p53 (TP53)-modified pigs may have an increased incidence of cancer. In this study, two pigs with mosaic mutations induced by gene editing were compared to determine the role of the wild-type TP53 se
Externí odkaz:
https://doaj.org/article/ebc72171b60d4c0086d62487ac252ce6
Autor:
Fuminori Tanihara, Maki Hirata, Zhao Namula, Lanh Thi Kim Do, Naoaki Yoshimura, Qingyi Lin, Koki Takebayashi, Tetsushi Sakuma, Takashi Yamamoto, Takeshige Otoi
Publikováno v:
Frontiers in Cell and Developmental Biology, Vol 11 (2023)
Just one amino acid at the carboxy-terminus of the B chain distinguishes human insulin from porcine insulin. By introducing a precise point mutation into the porcine insulin (INS) gene, we were able to generate genetically modified pigs that secreted
Externí odkaz:
https://doaj.org/article/3d3670a2590a45b290a7ac5ae1066968
Autor:
Maki Hirata, Manita Wittayarat, Zhao Namula, Quynh Anh Le, Qingyi Lin, Koki Takebayashi, Chommanart Thongkittidilok, Taro Mito, Sayuri Tomonari, Fuminori Tanihara, Takeshige Otoi
Publikováno v:
Scientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
Abstract The specificity and efficiency of CRISPR/Cas9 gene-editing systems are determined by several factors, including the mode of delivery, when applied to mammalian embryos. Given the limited time window for delivery, faster and more reliable met
Externí odkaz:
https://doaj.org/article/b7b32a20381743408ebe1d916513ccd3
Autor:
Qingyi Lin, Quynh Anh Le, Koki Takebayashi, Chommanart Thongkittidilok, Manita Wittayarat, Maki Hirata, Fuminori Tanihara, Takeshige Otoi
Publikováno v:
BMC Research Notes, Vol 14, Iss 1, Pp 1-6 (2021)
Abstract Objective Lipofection-mediated introduction of the CRISPR/Cas9 system in porcine zygotes provides a simple method for gene editing, without requiring micromanipulation. However, the gene editing efficiency is inadequate. The aim of this stud
Externí odkaz:
https://doaj.org/article/b83f00641d3b4b5b80130ae7c0521151
Autor:
Naoaki Yoshimura, Masayasu Taniguchi, Tsukasa Terazono, Tetsushi Ono, Mitsuhiro Takagi, Yoko Sato, Maki Hirata, Fuminori Tanihara, Takeshige Otoi
Publikováno v:
Veterinary Medicine and Science, Vol 7, Iss 5, Pp 1569-1574 (2021)
Abstract Follicular changes throughout the oestrous phase have been poorly documented in queens because of the location and the small size of ovaries. We investigated follicular development in queens treated with a combination of equine chorionic gon
Externí odkaz:
https://doaj.org/article/9510a1003b8c441bab628d38776cd5ef
Autor:
Zhao Namula, Manita Wittayarat, Lanh Thi Kim Do, Thanh Van Nguyen, Qingyi Lin, Koki Takebayashi, Maki Hirata, Fuminori Tanihara, Takeshige Otoi
Publikováno v:
Veterinary and Animal Science, Vol 16, Iss , Pp 100241- (2022)
Mosaicism, including alleles comprising both wild-type and mutant, is a serious problem for gene modification by gene editing using electroporation. One-step generation of F0 pigs with completely desired gene modifications saves cost and time, but th
Externí odkaz:
https://doaj.org/article/dc78bcb31d4843b4aea10f3a91957785