Development of three triplex real-time reverse transcription PCR assays for the qualitative molecular typing of the nine serotypes of African horse sickness virus.

Autor: Weyer CT; Equine Research Centre, Faculty of Veterinary Science, University of Pretoria, Private Bag X04, Onderstepoort 0110, South Africa. Electronic address: equineresearchcentre@gmail.com., Joone C; Equine Research Centre, Faculty of Veterinary Science, University of Pretoria, Private Bag X04, Onderstepoort 0110, South Africa., Lourens CW; Equine Research Centre, Faculty of Veterinary Science, University of Pretoria, Private Bag X04, Onderstepoort 0110, South Africa., Monyai MS; Equine Research Centre, Faculty of Veterinary Science, University of Pretoria, Private Bag X04, Onderstepoort 0110, South Africa., Koekemoer O; Agricultural Research Council, Onderstepoort Veterinary Institute, Onderstepoort 0110, South Africa; Department of Veterinary Tropical Diseases, Faculty of Veterinary Science, University of Pretoria, Private Bag X04, Onderstepoort 0110, South Africa., Grewar JD; Epidemiology, Veterinary Services (Animal Health) Western Cape Department of Agriculture, Private Bag X1, Elsenburg 7607, South Africa., van Schalkwyk A; Agricultural Research Council, Onderstepoort Veterinary Institute, Onderstepoort 0110, South Africa., Majiwa PO; Agricultural Research Council, Onderstepoort Veterinary Institute, Onderstepoort 0110, South Africa; Department of Veterinary Tropical Diseases, Faculty of Veterinary Science, University of Pretoria, Private Bag X04, Onderstepoort 0110, South Africa., MacLachlan NJ; Equine Viral Disease Laboratory, Department of Pathology, Microbiology and Immunology, School of Veterinary Medicine, University of California, Davis, CA 95616, USA; Department of Veterinary Tropical Diseases, Faculty of Veterinary Science, University of Pretoria, Private Bag X04, Onderstepoort 0110, South Africa., Guthrie AJ; Equine Research Centre, Faculty of Veterinary Science, University of Pretoria, Private Bag X04, Onderstepoort 0110, South Africa.

Publikováno v: Journal of virological methods [J Virol Methods] 2015 Oct; Vol. 223, pp. 69-74. Date of Electronic Publication: 2015 Jul 29.