Zobrazeno 1 - 10
of 15
pro vyhledávání: '"Maja Sidstedt"'
Publikováno v:
Biotechnology Reports, Vol 14, Iss C, Pp 34-37 (2017)
The success of real-time PCR (qPCR) analysis is partly limited by the presence of inhibitory compounds in the nucleic acid samples. For example, humic acid (HA) from soil and aqueous sediment interferes with amplification and also quenches the fluore
Externí odkaz:
https://doaj.org/article/ba3bdcfc116d4c478dbd30309be3ebd7
Publikováno v:
Forensic Science International: Genetics Supplement Series. 7:384-386
Methods for SNP-based phenotype prediction have recently been developed, but prediction accuracy data for several populations and regions are missing. We analysed the accuracy of hair and eye colour predictions for 111 individuals residing in Sweden,
Publikováno v:
Analytical and Bioanalytical Chemistry
DNA analysis has seen an incredible development in terms of instrumentation, assays and applications over the last years. Massively parallel sequencing (MPS) and digital PCR are now broadly applied in research and diagnostics, and quantitative PCR is
Autor:
Johannes Hedman, Peter Rådström, Peter M. Vallone, Erica L. Romsos, Maja Sidstedt, Leticia Waitara, Carolyn R. Steffen, Lars Wadsö
Publikováno v:
Analytical and Bioanalytical Chemistry
Blood samples are widely used for PCR-based DNA analysis in fields such as diagnosis of infectious diseases, cancer diagnostics, and forensic genetics. In this study, the mechanisms behind blood-induced PCR inhibition were evaluated by use of whole b
Autor:
Sascha Willuweit, Thorsten Hadrys, Lutz Roewer, Steffi Bredemeyer, Titia Sijen, Natalie E.C. Weiler, Sabrina Achtruth, Marc Trimborn, Ingo Bastisch, Christian Sell, F.-X. Laurent, Petra Müller, Walther Parson, Johannes Hedman, Maja Sidstedt
Publikováno v:
International Journal of Legal Medicine
We present results from an inter-laboratory massively parallel sequencing (MPS) study in the framework of the SeqForSTRs project to evaluate forensically relevant parameters, such as performance, concordance, and sensitivity, using a standardized seq
Autor:
Maja Sidstedt, Malin Sanga, Andreas O. Tillmar, Samuel Boiso, Ida Grandell, Johannes Hedman, Henrik Gréen
Publikováno v:
Forensic Science International: Genetics Supplement Series. 6:e267-e269
Massive parallel sequencing (MPS) is increasingly used for human identification purposes in forensic DNA laboratories. Forensic DNA samples are by nature heterogeneous and of varying quality, both concerning DNA integrity and matrices, creating a nee
Autor:
Maja Sidstedt, Johannes Hedman, Kevin M. Kiesler, Carolyn R. Steffen, Peter M. Vallone, Peter Rådström
Publikováno v:
Forensic science international. Genetics. 40
Massively parallel sequencing holds great promise for new possibilities in the field of forensic genetics, enabling simultaneous analysis of multiple markers as well as offering enhanced short tandem repeat allele resolution. A challenge in forensic
Autor:
Maja Sidstedt, Roman G. Gerlach, Martin B. Koeppel, Lubov Petkova Nedialkova, Lionello Bossi, Diana Ring, Stefanie Spriewald, Bärbel Stecher
Publikováno v:
Environmental Microbiology. 18:1591-1603
Bacteria employ bacteriocins for interference competition in microbial ecosystems. Colicin Ib (ColIb), a pore-forming bacteriocin, confers a significant fitness benefit to Salmonella enterica serovar Typhimurium (S. Tm) in competition against commens
Autor:
Elin Nilsson, Maja Sidstedt, Laila Noppa, Linda Jansson, Mats Forsman, Johannes Hedman, Peter Rådström
Publikováno v:
Analytical Biochemistry. 487:30-37
Real-time polymerase chain reaction (qPCR) is the cornerstone of DNA analysis, enabling detection and quantification of minute nucleic acid amounts. However, PCR-based analysis is limited, in part, by the presence of inhibitors in the samples. PCR in
Publikováno v:
Biotechnology Reports
Biotechnology Reports, Vol 14, Iss C, Pp 34-37 (2017)
Biotechnology Reports, Vol 14, Iss C, Pp 34-37 (2017)
Highlights • Humic acid quenches the fluorescence of the dsDNA binding dyes EvaGreen, ResoLight, SYBR Green I and SYTO9. • A four-dye blend containing the dyes above provides maximum fluorescence signals in presence and absence of humic acid. •