Design and validation of recombinant protein standards for quantitative Western blot analysis of cannabinoid CB1 receptor density in cell membranes: an alternative to radioligand binding methods

Autor: Miquel Saumell-Esnaola, Ainhoa Elejaga-Jimeno, Leyre Echeazarra, Leire Borrega-Román, Sergio Barrondo, Maider López de Jesús, Imanol González-Burguera, Alberto Gómez-Caballero, María Aranzazu Goicolea, Joan Sallés, Gontzal García del Caño

Publikováno v: Microbial Cell Factories, Vol 21, Iss 1, Pp 1-18 (2022)

Abstract Background Replacement of radioligand binding assays with antibody-antigen interaction-based approaches for quantitative analysis of G protein-coupled receptor (GPCR) levels requires the use of purified protein standards containing the antig

Externí odkaz: https://doaj.org/article/f211edb5792742e6b82bbb1f53d75237

Endocannabinoid 2-Arachidonoylglycerol Synthesis and Metabolism at Neuronal Nuclear Matrix Fractions Derived from Adult Rat Brain Cortex

Autor: Xabier Aretxabala, Gontzal García del Caño, Sergio Barrondo, Maider López de Jesús, Imanol González-Burguera, Miquel Saumell-Esnaola, María Aranzazu Goicolea, Joan Sallés

Publikováno v: International Journal of Molecular Sciences, Vol 24, Iss 4, p 3165 (2023)

In this report, we describe the kinetics characteristics of the diacylglycerol lipase-α (DGLα) located at the nuclear matrix of nuclei derived from adult cortical neurons. Thus, using high-resolution fluorescence microscopy, classical biochemical s

Externí odkaz: https://doaj.org/article/ed30659645da4597a7fe39685eced6eb

Effects of Platelet-Rich Plasma on Cellular Populations of the Central Nervous System: The Influence of Donor Age

Autor: Diego Delgado, Ane Miren Bilbao, Maider Beitia, Ane Garate, Pello Sánchez, Imanol González-Burguera, Amaia Isasti, Maider López De Jesús, Jone Zuazo-Ibarra, Alejandro Montilla, María Domercq, Estibaliz Capetillo-Zarate, Gontzal García del Caño, Joan Sallés, Carlos Matute, Mikel Sánchez

Publikováno v: International Journal of Molecular Sciences, Vol 22, Iss 4, p 1725 (2021)

Platelet-rich plasma (PRP) is a biologic therapy that promotes healing responses across multiple medical fields, including the central nervous system (CNS). The efficacy of this therapy depends on several factors such as the donor’s health status a

Externí odkaz: https://doaj.org/article/c11638bdf5d54d34bdc1616ad6c584d1

Highly efficient generation of glutamatergic/cholinergic NT2-derived postmitotic human neurons by short-term treatment with the nucleoside analogue cytosine β-d-arabinofuranoside

Autor: Imanol González-Burguera, Ana Ricobaraza, Xabier Aretxabala, Sergio Barrondo, Gontzal García del Caño, Maider López de Jesús, Joan Sallés

Publikováno v: Stem Cell Research, Vol 16, Iss 2, Pp 541-551 (2016)

The human NTERA2/D1 (NT2) cells generate postmitotic neurons (NT2N cells) upon retinoic acid (RA) treatment and are functionally integrated in the host tissue following grafting into the rodent and human brain, thus representing a promising source fo

Externí odkaz: https://doaj.org/article/0ad9ce0eb2f149a78cd9ec004392ee29

Teratokartzinometatik eratorritako zelula-lerro neuronalen (NT2N) transplantea: istripu zerebrobaskularra izandako gaixo kronikoentzako estrategia terapeutiko berria

Autor: Amaia Isasti, Sergio Barrondo, Miquel Saumell, Gontzal García de caño, Imanol González-Burguera, Joan Sallés, Maider López de Jesús, Leire Borrega

Publikováno v: Addi. Archivo Digital para la Docencia y la Investigación
instname
Addi: Archivo Digital para la Docencia y la Investigación
Universidad del País Vasco

Currently, Cerebrovascular accident (CVA) also known as stroke, is the third leading cause of disability and death worldwide. CVA is a sudden alteration of cerebral circulation, which results in neural cell death and consequently a temporary or perma

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::31ac04c718d37e9bf495c7fbcf7205af
https://doi.org/10.1387/ekaia.21296