Opto-RhoGEFs, an optimized optogenetic toolbox to reversibly control Rho GTPase activity on a global to subcellular scale, enabling precise control over vascular endothelial barrier strength.

Autor: Mahlandt EK; Swammerdam Institute for Life Sciences, Section of Molecular Cytology, van Leeuwenhoek Centre for Advanced Microscopy, University of Amsterdam, Amsterdam, Netherlands., Palacios Martínez S; Swammerdam Institute for Life Sciences, Section of Molecular Cytology, van Leeuwenhoek Centre for Advanced Microscopy, University of Amsterdam, Amsterdam, Netherlands., Arts JJG; Swammerdam Institute for Life Sciences, Section of Molecular Cytology, van Leeuwenhoek Centre for Advanced Microscopy, University of Amsterdam, Amsterdam, Netherlands.; Molecular Cell Biology Lab at Dept. Molecular Hematology, Sanquin Research and Landsteiner Laboratory, Amsterdam, Netherlands., Tol S; Molecular Cell Biology Lab at Dept. Molecular Hematology, Sanquin Research and Landsteiner Laboratory, Amsterdam, Netherlands., van Buul JD; Swammerdam Institute for Life Sciences, Section of Molecular Cytology, van Leeuwenhoek Centre for Advanced Microscopy, University of Amsterdam, Amsterdam, Netherlands.; Molecular Cell Biology Lab at Dept. Molecular Hematology, Sanquin Research and Landsteiner Laboratory, Amsterdam, Netherlands., Goedhart J; Swammerdam Institute for Life Sciences, Section of Molecular Cytology, van Leeuwenhoek Centre for Advanced Microscopy, University of Amsterdam, Amsterdam, Netherlands.

Publikováno v: ELife [Elife] 2023 Jul 14; Vol. 12. Date of Electronic Publication: 2023 Jul 14.

ARHGAP17 regulates the spatiotemporal activity of Cdc42 at invadopodia.

Autor: Kreider-Letterman G; Department of Biological Sciences, University of Toledo, Toledo, OH, USA., Castillo A; Department of Biological Sciences, University of Toledo, Toledo, OH, USA., Mahlandt EK; Swammerdam Institute for Life Sciences, Section of Molecular Cytology, van Leeuwenhoek Centre for Advanced Microscopy, University of Amsterdam, Amsterdam, The Netherlands., Goedhart J; Swammerdam Institute for Life Sciences, Section of Molecular Cytology, van Leeuwenhoek Centre for Advanced Microscopy, University of Amsterdam, Amsterdam, The Netherlands., Rabino A; Department of Biological Sciences, University of Toledo, Toledo, OH, USA., Goicoechea S; Department of Biological Sciences, University of Toledo, Toledo, OH, USA., Garcia-Mata R; Department of Biological Sciences, University of Toledo, Toledo, OH, USA.

Publikováno v: The Journal of cell biology [J Cell Biol] 2023 Feb 06; Vol. 222 (2). Date of Electronic Publication: 2022 Dec 26.

A turquoise fluorescence lifetime-based biosensor for quantitative imaging of intracellular calcium.

Autor: van der Linden FH; Section of Molecular Cytology, van Leeuwenhoek Centre for Advanced Microscopy, Swammerdam Institute for Life Sciences, University of Amsterdam, Amsterdam, The Netherlands., Mahlandt EK; Section of Molecular Cytology, van Leeuwenhoek Centre for Advanced Microscopy, Swammerdam Institute for Life Sciences, University of Amsterdam, Amsterdam, The Netherlands., Arts JJG; Section of Molecular Cytology, van Leeuwenhoek Centre for Advanced Microscopy, Swammerdam Institute for Life Sciences, University of Amsterdam, Amsterdam, The Netherlands.; Department of Molecular Hematology at Sanquin Research and Landsteiner Laboratory, Academic Medical Centre, University of Amsterdam, Amsterdam, The Netherlands., Beumer J; Oncode Institute, Hubrecht Institute, Royal Netherlands Academy of Arts and Sciences and University Medical Center, Utrecht, The Netherlands., Puschhof J; Oncode Institute, Hubrecht Institute, Royal Netherlands Academy of Arts and Sciences and University Medical Center, Utrecht, The Netherlands., de Man SMA; Section of Molecular Cytology, van Leeuwenhoek Centre for Advanced Microscopy, Swammerdam Institute for Life Sciences, University of Amsterdam, Amsterdam, The Netherlands., Chertkova AO; Section of Molecular Cytology, van Leeuwenhoek Centre for Advanced Microscopy, Swammerdam Institute for Life Sciences, University of Amsterdam, Amsterdam, The Netherlands., Ponsioen B; Center for Molecular Medicine, Oncode Institute, University Medical Centre Utrecht, Utrecht, The Netherlands., Clevers H; Oncode Institute, Hubrecht Institute, Royal Netherlands Academy of Arts and Sciences and University Medical Center, Utrecht, The Netherlands., van Buul JD; Section of Molecular Cytology, van Leeuwenhoek Centre for Advanced Microscopy, Swammerdam Institute for Life Sciences, University of Amsterdam, Amsterdam, The Netherlands.; Department of Molecular Hematology at Sanquin Research and Landsteiner Laboratory, Academic Medical Centre, University of Amsterdam, Amsterdam, The Netherlands., Postma M; Section of Molecular Cytology, van Leeuwenhoek Centre for Advanced Microscopy, Swammerdam Institute for Life Sciences, University of Amsterdam, Amsterdam, The Netherlands., Gadella TWJ Jr; Section of Molecular Cytology, van Leeuwenhoek Centre for Advanced Microscopy, Swammerdam Institute for Life Sciences, University of Amsterdam, Amsterdam, The Netherlands., Goedhart J; Section of Molecular Cytology, van Leeuwenhoek Centre for Advanced Microscopy, Swammerdam Institute for Life Sciences, University of Amsterdam, Amsterdam, The Netherlands. j.goedhart@uva.nl.

Publikováno v: Nature communications [Nat Commun] 2021 Dec 09; Vol. 12 (1), pp. 7159. Date of Electronic Publication: 2021 Dec 09.

Endothelial junctional membrane protrusions serve as hotspots for neutrophil transmigration.

Autor: Arts JJ; Molecular Cell Biology Lab at Dept. Molecular Hematology, Sanquin Research and Landsteiner Laboratory, Amsterdam, Netherlands.; Leeuwenhoek Centre for Advanced Microscopy (LCAM), section Molecular Cytology at Swammerdam Institute for Life Sciences (SILS) at University of Amsterdam, Amsterdam, Netherlands., Mahlandt EK; Leeuwenhoek Centre for Advanced Microscopy (LCAM), section Molecular Cytology at Swammerdam Institute for Life Sciences (SILS) at University of Amsterdam, Amsterdam, Netherlands., Grönloh ML; Molecular Cell Biology Lab at Dept. Molecular Hematology, Sanquin Research and Landsteiner Laboratory, Amsterdam, Netherlands.; Leeuwenhoek Centre for Advanced Microscopy (LCAM), section Molecular Cytology at Swammerdam Institute for Life Sciences (SILS) at University of Amsterdam, Amsterdam, Netherlands., Schimmel L; Molecular Cell Biology Lab at Dept. Molecular Hematology, Sanquin Research and Landsteiner Laboratory, Amsterdam, Netherlands.; Leeuwenhoek Centre for Advanced Microscopy (LCAM), section Molecular Cytology at Swammerdam Institute for Life Sciences (SILS) at University of Amsterdam, Amsterdam, Netherlands.; Division of Cell and Developmental Biology, Institute for Molecular Bioscience, The University of Queensland, BrisbaneQLD, Australia., Noordstra I; Division of Cell and Developmental Biology, Institute for Molecular Bioscience, The University of Queensland, BrisbaneQLD, Australia., Gordon E; Division of Cell and Developmental Biology, Institute for Molecular Bioscience, The University of Queensland, BrisbaneQLD, Australia., van Steen AC; Molecular Cell Biology Lab at Dept. Molecular Hematology, Sanquin Research and Landsteiner Laboratory, Amsterdam, Netherlands., Tol S; Molecular Cell Biology Lab at Dept. Molecular Hematology, Sanquin Research and Landsteiner Laboratory, Amsterdam, Netherlands., Walzog B; Department of Cardiovascular Physiology and Pathophysiology, Walter Brendel Center of Experimental Medicine, Biomedical Center, Ludwig-Maximilians-Universität München, Planegg-Martinsried, Germany., van Rijssel J; Molecular Cell Biology Lab at Dept. Molecular Hematology, Sanquin Research and Landsteiner Laboratory, Amsterdam, Netherlands., Nolte MA; Molecular Cell Biology Lab at Dept. Molecular Hematology, Sanquin Research and Landsteiner Laboratory, Amsterdam, Netherlands., Postma M; Leeuwenhoek Centre for Advanced Microscopy (LCAM), section Molecular Cytology at Swammerdam Institute for Life Sciences (SILS) at University of Amsterdam, Amsterdam, Netherlands., Khuon S; Advanced Imaging Center at Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, United States., Heddleston JM; Advanced Imaging Center at Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, United States.; Microscopy Facility at the Cleveland Clinic Florida Research and Innovation Center, Port St. Lucie, United States., Wait E; Advanced Imaging Center at Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, United States., Chew TL; Advanced Imaging Center at Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, United States., Winter M; Zuckerman Postdoctoral Fellow, Department of Marine Sciences, University of Haifa, Haifa, Israel., Montanez E; Department of Physiological Sciences, Faculty of Medicine and Health Sciences, University of Barcelona, Barcelona, Spain., Goedhart J; Leeuwenhoek Centre for Advanced Microscopy (LCAM), section Molecular Cytology at Swammerdam Institute for Life Sciences (SILS) at University of Amsterdam, Amsterdam, Netherlands., van Buul JD; Molecular Cell Biology Lab at Dept. Molecular Hematology, Sanquin Research and Landsteiner Laboratory, Amsterdam, Netherlands.; Leeuwenhoek Centre for Advanced Microscopy (LCAM), section Molecular Cytology at Swammerdam Institute for Life Sciences (SILS) at University of Amsterdam, Amsterdam, Netherlands.

Publikováno v: ELife [Elife] 2021 Aug 25; Vol. 10. Date of Electronic Publication: 2021 Aug 25.