Expression, functional characterization, and preliminary crystallization of the cochaperone prefoldin from the thermophilic fungus chaetomium thermophilum

Autor: Ayaka Hori, Keiichi Noguchi, Yuko Uno, Kentaro Ishii, Mahito Kikumoto, Kento Morita, Teru Ogura, Kentaro Noi, Tomohiro Obata, Rocío Arranz, José M. Valpuesta, Masafumi Yohda, Yohei Y. Yamamoto, Kyosuke Shinohara, Koichi Kato

Publikováno v: Digital.CSIC. Repositorio Institucional del CSIC
instname
International Journal of Molecular Sciences, Vol 19, Iss 8, p 2452 (2018)
International Journal of Molecular Sciences
Volume 19
Issue 8

Prefoldin is a hexameric molecular chaperone found in the cytosol of archaea and eukaryotes. Its hexameric complex is built from two related classes of subunits, and has the appearance of a jellyfish: Its body consists of a double β-barrel assembly

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9ddc8310045130c175b975f1b1d93e90
http://hdl.handle.net/10261/169014

Effects of the KIF2C neck peptide on microtubules: lateral disintegration of microtubules and β-structure formation

Autor: Masayuki Nara, Hiroaki Kojima, Mahito Kikumoto, Hisayuki Morii, Takashi Shimizu, Youské Shimizu

Publikováno v: FEBS Journal. 280:1681-1692

Members of the kinesin-13 sub-family, including KIF2C, depolymerize microtubules. The positive charge-rich 'neck' region extending from the N-terminus of the catalytic head is considered to be important in the depolymerization activity. Chemically sy

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6796991dfa72c51803fab296ba2fb5cd
https://doi.org/10.1111/febs.12182

Correlation between the Activities and the Oligomeric Forms of Pig Gastric H/K-ATPase

Autor: Kazuhiro Oiwa, Shunji Kaya, Toshiaki Imagawa, Yutaro Hayashi, Michio Yazawa, Kazuhiro Abe, Mahito Kikumoto, Kazuya Taniguchi, Tsuyoshi Katoh

Publikováno v: Biochemistry. 42:15132-15138

Membrane-bound H/K-ATPase was solubilized by octaethylene glycol dodecyl ether (C(12)E(8)) or n-octyl glucoside (nOG). H/K-ATPase activity and the distribution of protomeric and oligomeric components were evaluated by high-performance gel chromatogra

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6013806b5ba4f4c04c2f9fa1bde00fd3
https://doi.org/10.1021/bi035686x

Direct visualization and characterization of stable microtubules from the neurites of cultured dorsal root ganglion cells

Autor: Mahito Kikumoto, Tomoko Tashiro, Yoshiaki Komiya, Hideo Tashiro, Masashi Kurachi

Publikováno v: Journal of Neuroscience Research. 50:81-93

We tested the stability of microtubules (MTs) in the neurites of cultured dorsal root ganglion cells by dissolving the cytoplasmic membrane with detergent and exposing them to defined extracellular medium under observation with a video-enhanced diffe

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0b2dcf4325927f081b756cfe925349de
https://doi.org/10.1002/(sici)1097-4547(19971001)50:1<81::aid-jnr9>3.0.co

Evidence for a relationship between activity and the tetraprotomeric assembly of solubilized pig gastric H/K-ATPase

Autor: Toshiaki Imagawa, Kazuya Taniguchi, Yutaro Hayashi, Shunji Kaya, Kazuhiro Abe, Kazuyasu Sakaguchi, Mahito Kikumoto, Kazuhiro Oiwa

Publikováno v: Journal of biochemistry. 138(3)

Activity-oligomeric assembly relationships using octaethylene glycol dodecyl ether (C 1 2 E 8 ) solubilized pig gastric H/K-ATPase (unmodified H/K-ATPase) or H/K-ATPase modified with Fluorescein 5'-isothiocyanate (FITC-H/K-ATPase) were examined. The

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e4e8b5f7788d388d16361980dfb40dae
https://pubmed.ncbi.nlm.nih.gov/16169880