Importance of methionine biosynthesis for Arabidopsis seed germination and seedling growth

Autor: Dominique Job, Claudette Job, Hans Demol, Joël Vandekerckhove, Magda Puype, Karine Gallardo, Steven P.C. Groot

Publikováno v: Physiologia Plantarum, 116(2), 238-247
Physiologia Plantarum 116 (2002) 2

Proteomics of Arabidopsis seeds revealed the differential accumulation during germination of two housekeeping enzymes. The first corresponded to methionine synthase that catalyses the last step in the plant methionine biosynthetic pathway. This prote

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::993b47d8940116caf7f146892a9e0b4a
https://doi.org/10.1034/j.1399-3054.2002.1160214.x

Peptides adsorbed on reverse-phase chromatographic beads as targets for femtomole sequencing by post-source decay matrix assisted laser desorption ionization-reflectron time of flight mass spectrometry (MALDI-RETOF-MS)

Autor: Daniël Broekaert, Tony Houthaeve, Kris Gevaert, Hans Demol, Joël Vandekerckhove, Magda Puype, Stefaan De Boeck

Publikováno v: Electrophoresis. 18:2950-2960

We here describe a procedure for concentrating peptides from solutions by adsorbing them onto reverse-phase beads that were added to these solutions. The beads are then transferred to the target disc of the matrix assisted laser desorption ionization

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1449fc45e1d91687803a716bab70caeb
https://doi.org/10.1002/elps.1150181537

Structural analysis and identification of gel-purified proteins, available in the femtomole range, using a novel computer program for peptide sequence assignment, by matrix-assisted laser desorption ionization — reflectron time-of-fligh — mass spectrometry

Autor: Stefaan De Boeck, Joël Vandekerckhove, Magda Puype, Kris Gevaert, Jean-Luc Verschelde, Jozef Van Damme, Marc Goethals

Publikováno v: Electrophoresis. 17:918-924

A procedure is described for structural characterization and identification of proteins, purified by either one- or two-dimensional gel electrophoresis in the low picomole to femtomole range. The purified proteins are first detected in the primary ge

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::96df584c811fb84e13f5f5d648715bb6
https://doi.org/10.1002/elps.1150170513

Differential substrate specificity of group I and group II chaperonins in the archaeon Methanosarcina mazei

Autor: Francesca Forner, Burghardt Scheibe, F. Ulrich Hartl, Joël Vandekerckhove, Magda Puype, Matthias Mann, Angela Hirtreiter, Giulia Calloni, Manajit Hayer-Hartl

Publikováno v: Molecular microbiology. 74(5)

Chaperonins are macromolecular machines that assist in protein folding. The archaeon Methanosarcina mazei has acquired numerous bacterial genes by horizontal gene transfer. As a result, both the bacterial group I chaperonin, GroEL, and the archaeal g

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::75751d627c06e9782bdb05eb07c88a63
https://pubmed.ncbi.nlm.nih.gov/19843217

The actin propulsive machinery: the proteome of Listeria monocytogenes tails

Autor: Marleen Van Troys, Violaine David, Pascale Cossart, Kris Gevaert, Javier Pizarro-Cerdá, Hans Demol, Joël Vandekerckhove, Magda Puype, Anja Lambrechts

Publikováno v: Biochemical and Biophysical Research Communications
Biochemical and Biophysical Research Communications, Elsevier, 2008, 375, pp.194-199. ⟨10.1016/j.bbrc.2008.07.152⟩
Biochemical and Biophysical Research Communications, 2008, 375, pp.194-199. ⟨10.1016/j.bbrc.2008.07.152⟩

International audience; Actin-based comet tails produced by Listeria monocytogenes are considered as representative models for cellular force-producing machineries crucial for cell migration. We here present a proteomic picture of these tails formed

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ad5de5ebb32bd9f663f5d426987b4b14
https://pubmed.ncbi.nlm.nih.gov/18694727