Výsledky vyhledávání

Proteome analysis of human mesothelial cells during epithelial to mesenchymal transitions induced by shed menstrual effluent

Autor: Patrick G. Groothuis, Andreas Herrler, Ayşe Y. Demir, Gerard A.J. Dunselman, Anton F.P.M. de Goeij, Johannes L.H. Evers, Hans Demol, Joël Vandekerckhove, Magda Puype

Publikováno v: PROTEOMICS. 4:2608-2623

Peritoneal endometriosis is the result of ectopic implantation and growth of endometrium tissue that has been regurgitated into the abdominal cavity during menstruation. We have previously shown that menstrual effluent induces epithelial to mesenchym

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d91a7bfa0fd7cd406cf2305597af3799
https://doi.org/10.1002/pmic.200300827

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Chromatographic Isolation of Methionine-containing Peptides for Gel-free Proteome Analysis

Autor: Jozef Van Damme, An Staes, Marc Goethals, Bart Hoorelbeke, Lennart Martens, Hans Demol, Joël Vandekerckhove, Kris Gevaert, Magda Puype, Grégoire Thomas

Publikováno v: Molecular & Cellular Proteomics. 1:896-903

A novel gel-free proteomic technology was used to identify more than 800 proteins from 50 million Escherichia coli K12 cells in a single analysis. A peptide mixture is first obtained from a total unfractionated cell lysate, and only the methionine-co

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::d00ba932863cc3249a519a3b557eb675
https://doi.org/10.1074/mcp.m200061-mcp200

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Importance of methionine biosynthesis for Arabidopsis seed germination and seedling growth

Autor: Dominique Job, Claudette Job, Hans Demol, Joël Vandekerckhove, Magda Puype, Karine Gallardo, Steven P.C. Groot

Publikováno v: Physiologia Plantarum, 116(2), 238-247
Physiologia Plantarum 116 (2002) 2

Proteomics of Arabidopsis seeds revealed the differential accumulation during germination of two housekeeping enzymes. The first corresponded to methionine synthase that catalyses the last step in the plant methionine biosynthetic pathway. This prote

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::993b47d8940116caf7f146892a9e0b4a
https://doi.org/10.1034/j.1399-3054.2002.1160214.x

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Identification by redox proteomics of glutathionylated proteins in oxidatively stressed human T lymphocytes

Autor: Mario Salmona, Angela Bachi, Maddalena Fratelli, Simona Casagrande, Ivano Eberini, Emeric Miclet, Francis Duffieux, Valentina Bonetto, Hans Demol, Joël Vandekerckhove, Magda Puype, Manuela Mengozzi, Pietro Ghezzi, Elisabetta Gianazza

Publikováno v: Proceedings of the National Academy of Sciences. 99:3505-3510

Formation of mixed disulfides between glutathione and the cysteines of some proteins (glutathionylation) has been suggested as a mechanism through which protein functions can be regulated by the redox status. The aim of this study was to identify the

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a1e9fc6b4f5a636cebd3ac45eaf33344
https://doi.org/10.1073/pnas.052592699

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Peptides adsorbed on reverse-phase chromatographic beads as targets for femtomole sequencing by post-source decay matrix assisted laser desorption ionization-reflectron time of flight mass spectrometry (MALDI-RETOF-MS)

Autor: Daniël Broekaert, Tony Houthaeve, Kris Gevaert, Hans Demol, Joël Vandekerckhove, Magda Puype, Stefaan De Boeck

Publikováno v: Electrophoresis. 18:2950-2960

We here describe a procedure for concentrating peptides from solutions by adsorbing them onto reverse-phase beads that were added to these solutions. The beads are then transferred to the target disc of the matrix assisted laser desorption ionization

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1449fc45e1d91687803a716bab70caeb
https://doi.org/10.1002/elps.1150181537

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Molecular characterization of the human peroxisomal branchedchain acyl-CoA oxidase: cDNA cloning, chromosomal assignment, tissue distribution, and evidence for the absence of the protein in Zellweger syndrome

Autor: H. Dariush Fahimi, Johannes C. T. Vanhooren, M. Fransen, Paul P. Van Veldhoven, Joël Vandekerckhove, Magda Puype, Peter Marynen, Jack A. M. Leunissen, Eveline Baumgart, Guy P. Mannaerts

Publikováno v: Proceedings of the National Academy of Sciences. 93:13748-13753

Peroxisomes in human liver contain two distinct acyl-CoA oxidases with different substrate specificities: ( i ) palmitoyl-CoA oxidase, oxidizing very long straight-chain fatty acids and eicosanoids, and ( ii ) a branched-chain acyl-CoA oxidase (hBRCA

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ef2b63e9483d7afe03b6dde3abd5b98b
https://doi.org/10.1073/pnas.93.24.13748

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Structural analysis and identification of gel-purified proteins, available in the femtomole range, using a novel computer program for peptide sequence assignment, by matrix-assisted laser desorption ionization — reflectron time-of-fligh — mass spectrometry

Autor: Stefaan De Boeck, Joël Vandekerckhove, Magda Puype, Kris Gevaert, Jean-Luc Verschelde, Jozef Van Damme, Marc Goethals

Publikováno v: Electrophoresis. 17:918-924

A procedure is described for structural characterization and identification of proteins, purified by either one- or two-dimensional gel electrophoresis in the low picomole to femtomole range. The purified proteins are first detected in the primary ge

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::96df584c811fb84e13f5f5d648715bb6
https://doi.org/10.1002/elps.1150170513

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The human keratinocyte two-dimensional gel protein database: Update 1993

Publikováno v: Electrophoresis. 14:1091-1097

The master two-dimensional gel database of human keratinocytes currently lists 3038 cellular proteins (2127 isoelectric focusing, IEF; and 911 nonequilibrium pH gradient electrophoresis, NEPHGE) many of which correspond to post-translational modifica

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::27a16564e035d9fb768836b5782591a1
https://doi.org/10.1002/elps.11501401178

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Differential substrate specificity of group I and group II chaperonins in the archaeon Methanosarcina mazei

Autor: Francesca Forner, Burghardt Scheibe, F. Ulrich Hartl, Joël Vandekerckhove, Magda Puype, Matthias Mann, Angela Hirtreiter, Giulia Calloni, Manajit Hayer-Hartl

Publikováno v: Molecular microbiology. 74(5)

Chaperonins are macromolecular machines that assist in protein folding. The archaeon Methanosarcina mazei has acquired numerous bacterial genes by horizontal gene transfer. As a result, both the bacterial group I chaperonin, GroEL, and the archaeal g

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::75751d627c06e9782bdb05eb07c88a63
https://pubmed.ncbi.nlm.nih.gov/19843217

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Microsequencing of proteins recorded in human two-dimensional gel protein databases

Autor: Josef Van Damme, Joël Vandekerckhove, Magda Puype, Julio E. Celis, Borbala Gesser, Hanne H. Rasmussen

Publikováno v: Rasmussen, MK, Van Damme, J, Puype, M, Gesser, B, Celis, J E & Vandekerckhove, J 1991, ' Microsequencing of proteins recorded in human two-dimensional gel protein databases ', Electrophoresis, vol. 12, no. 11, pp. 873-82 . https://doi.org/10.1002/elps.1150121107

Sixty-six human proteins recorded in the master transformed human epithelial amnion cells (AMA) (55) and keratinocyte (11) two-dimensional gel protein databases have been microsequenced since the last publication of the AMA database (Electrophoresis

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e592a1a29d772405ca6c3197c88bf2d0
https://doi.org/10.1002/elps.1150121107

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