Zobrazeno 1 - 10
of 74
pro vyhledávání: '"Mag-Fura 2"'
Publikováno v:
European Journal of Histochemistry, Vol 60, Iss 3 (2016)
We propose a tool for a rapid high-resolution detection of calcium ions which can be used in parallel with other techniques. We have applied a new approach by photo-oxidation of diaminobenzidine in presence of the emission of an excited fluorochrome
Externí odkaz:
https://doaj.org/article/26651cb1b68f448391cb5afeed59d6fa
Akademický článek
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Autor:
Marco Biggiogera, Germano Guerra, Vittorio Rosti, V. Galimberti, Valentina Poletto, Francesco Moccia
Publikováno v:
European Journal of Histochemistry : EJH
European Journal of Histochemistry, Vol 60, Iss 3 (2016)
European Journal of Histochemistry, Vol 60, Iss 3 (2016)
We propose a tool for a rapid high-resolution detection of calcium ions which can be used in parallel with other techniques. We have applied a new approach by photo-oxidation of diaminobenzidine in presence of the emission of an excited fluorochrome
Autor:
Stefano Iotti, Jörg R. Aschenbach, Nasrin Abdulhanan, Alina Smorodchenko, Zheng Zhang, Gerhard Sponder, Katharina Wolf, Lucia Mastrototaro, Jürgen Vormann, Andrea Fleig, Lucia Merolle, Reinhold Penner, Martin Kolisek, Katharina Kurth
CNNM2 is associated with the regulation of serum Mg concentration, and when mutated, with severe familial hypomagnesemia. The function and cellular localization of CNNM2 and its isomorphs (Iso) remain controversial. The objective of this work was to
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5c1f06755a831afff55e4648ef7e6d53
http://hdl.handle.net/11585/587740
http://hdl.handle.net/11585/587740
Publikováno v:
Biochimica et Biophysica Acta (BBA) - Biomembranes. 1618(1):1-7
Arachidonic acid (AA), a metabolite of membrane phospholipids, and its metabolites are increased in Mg2+ deficiency. We examined whether the extracellular Mg2+ concentration affects AA production and whether AA regulates a putative Na+-dependent Mg2+
Publikováno v:
Journal of Fluorescence. 9:103-110
A test based on time-resolved fluorescence experiments (Anal. Biochem.245, 28–37, 1997) allows one to assess the interference of the excited-state association with the fluorimetric determination of the ground-state dissociation constant Kd of fluor
Autor:
Helen J. Kennedy
Publikováno v:
Experimental Physiology. 83:449-460
The extrusion mechanism for intracellular Mg 2+ was investigated in voltage-clamped snail neurones using Mg 2+ -sensitive microelectrodes and mag-fura-2. The intracellular free magnesium ion concentration ([Mg 2+ ] i ) of snail neurones voltage clamp
Publikováno v:
Chemical Physics Letters. 287:412-420
Mag-fura-2 is the first commercial fluorescent indicator for the determination of intracellular Mg 2+ concentrations. To investigate the reversible reaction of Mg 2+ with this indicator in the ground and excited states, steady-state and time-resolved
Publikováno v:
Biochimica et Biophysica Acta (BBA) - Biomembranes. 1192:281-285
Polyamines, particularly spermine, in physiological concentrations interact with mag-fura-2 and the mag-fura-2/Mg2+ complex, resulting in reduced values of free Mg2+ concentration. Similarly, polyamines interact with ATP and MgATP. Thus, free Mg2+ co
Publikováno v:
Journal of Cellular and Molecular Medicine
The human gene MRS2L encodes a mitochondrial protein distantly related to CorA Mg(2+) transport proteins. Constitutive shRNA-mediated knockdown of hMRS2 in human HEK-293 cell line was found here to cause death. To further study its role in Mg(2+) tra