Cysteine dependence in Lactobacillus iners constitutes a novel therapeutic target to modify the vaginal microbiota

Autor: Alissa J. Mitchell, Mafunda Na, Ceasar X, Benjamin M. Woolston, J.F. Frempong, Caroline M. Mitchell, J.K. Rice, Mary Dong, Douglas S. Kwon, N. Choksi, Musie Ghebremichael, Seth M. Bloom, Fatima A. Hussain, Gumbi T, Nasreen Ismail, Thumbi Ndung'u, Emily P. Balskus, X. Westergaard, Nondumiso Xulu, Jiawu Xu, Krista L. Dong, Abai Ab, Matthew R. Hayward

Vaginal microbiota composition affects several important reproductive health outcomes. Lactobacillus crispatus-dominant bacterial communities have favorable associations whereas anaerobe-dominant communities deficient of lactobacilli are linked to po

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::539a6a807cae5223eeb770685b60d4f6
https://doi.org/10.1101/2021.06.12.448098

Cysteine dependence of Lactobacillus iners is a potential therapeutic target for vaginal microbiota modulation.

Autor: Bloom SM; Ragon Institute of MGH, MIT and Harvard, Cambridge, MA, USA.; Division of Infectious Diseases, Massachusetts General Hospital, Boston, MA, USA.; Harvard Medical School, Boston, MA, USA., Mafunda NA; Ragon Institute of MGH, MIT and Harvard, Cambridge, MA, USA.; Harvard T.H. Chan School of Public Health, Boston, MA, USA., Woolston BM; Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA, USA.; Department of Chemical Engineering, Northeastern University, Boston, MA, USA., Hayward MR; Ragon Institute of MGH, MIT and Harvard, Cambridge, MA, USA.; Harvard Medical School, Boston, MA, USA., Frempong JF; Ragon Institute of MGH, MIT and Harvard, Cambridge, MA, USA.; Medical Scientist Training Program, Washington University School of Medicine, St Louis, MO, USA., Abai AB; Harvard College, Harvard University, Cambridge, MA, USA., Xu J; Ragon Institute of MGH, MIT and Harvard, Cambridge, MA, USA., Mitchell AJ; Department of Obstetrics and Gynecology, Massachusetts General Hospital, Boston, MA, USA.; William Carey University College of Osteopathic Medicine, Hattiesburg, MS, USA., Westergaard X; Ragon Institute of MGH, MIT and Harvard, Cambridge, MA, USA.; Department of Obstetrics and Gynecology, Massachusetts General Hospital, Boston, MA, USA.; Department of Biological Sciences, Columbia University, New York, NY, USA., Hussain FA; Ragon Institute of MGH, MIT and Harvard, Cambridge, MA, USA.; Harvard Medical School, Boston, MA, USA., Xulu N; HIV Pathogenesis Programme (HPP), The Doris Duke Medical Research Institute, University of KwaZulu-Natal, Durban, South Africa., Dong M; Ragon Institute of MGH, MIT and Harvard, Cambridge, MA, USA.; Massachusetts General Hospital, Boston, MA, USA., Dong KL; Ragon Institute of MGH, MIT and Harvard, Cambridge, MA, USA.; Division of Infectious Diseases, Massachusetts General Hospital, Boston, MA, USA.; Harvard Medical School, Boston, MA, USA., Gumbi T; Health Systems Trust, Durban, South Africa., Ceasar FX; Health Systems Trust, Durban, South Africa., Rice JK; Harvard Medical School, Boston, MA, USA.; Ronald O. Perelman Department of Emergency Medicine, NYU School of Medicine, New York, NY, USA., Choksi N; Department of Obstetrics and Gynecology, Massachusetts General Hospital, Boston, MA, USA.; Rishihood University - School of Healthcare, Sonepat, Haryana, India., Ismail N; HIV Pathogenesis Programme (HPP), The Doris Duke Medical Research Institute, University of KwaZulu-Natal, Durban, South Africa., Ndung'u T; Ragon Institute of MGH, MIT and Harvard, Cambridge, MA, USA.; HIV Pathogenesis Programme (HPP), The Doris Duke Medical Research Institute, University of KwaZulu-Natal, Durban, South Africa.; Africa Health Research Institute (AHRI), Durban, South Africa.; Max Planck Institute for Infection Biology, Berlin, Germany.; Division of Infection and Immunity, University College London, London, UK., Ghebremichael MS; Ragon Institute of MGH, MIT and Harvard, Cambridge, MA, USA.; Harvard Medical School, Boston, MA, USA., Relman DA; Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, CA, USA.; Department of Medicine, Stanford University School of Medicine, Stanford, CA, USA.; Infectious Diseases Section, Veterans Affairs Palo Alto Health Care System, Palo Alto, CA, USA., Balskus EP; Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA, USA., Mitchell CM; Ragon Institute of MGH, MIT and Harvard, Cambridge, MA, USA.; Harvard Medical School, Boston, MA, USA.; Department of Obstetrics and Gynecology, Massachusetts General Hospital, Boston, MA, USA., Kwon DS; Ragon Institute of MGH, MIT and Harvard, Cambridge, MA, USA. dkwon@mgh.harvard.edu.; Division of Infectious Diseases, Massachusetts General Hospital, Boston, MA, USA. dkwon@mgh.harvard.edu.; Harvard Medical School, Boston, MA, USA. dkwon@mgh.harvard.edu.

Publikováno v: Nature microbiology [Nat Microbiol] 2022 Mar; Vol. 7 (3), pp. 434-450. Date of Electronic Publication: 2022 Mar 03.

Antigen Presenting Cells Link the Female Genital Tract Microbiome to Mucosal Inflammation, With Hormonal Contraception as an Additional Modulator of Inflammatory Signatures.

Autor: Byrne EH; Ragon Institute of Massachusetts General Hospital (MGH), Massachusetts Institute of Technology (MIT), and Harvard, Cambridge, MA, United States.; Department of Medicine, Harvard Medical School, Boston, MA, United States., Farcasanu M; Ragon Institute of Massachusetts General Hospital (MGH), Massachusetts Institute of Technology (MIT), and Harvard, Cambridge, MA, United States., Bloom SM; Ragon Institute of Massachusetts General Hospital (MGH), Massachusetts Institute of Technology (MIT), and Harvard, Cambridge, MA, United States.; Department of Medicine, Harvard Medical School, Boston, MA, United States.; Division of Infectious Diseases, Massachusetts General Hospital, Boston, MA, United States., Xulu N; HIV Pathogenesis Programme (HPP), The Doris Duke Medical Research Institute, University of KwaZulu-Natal, Durban, South Africa., Xu J; Ragon Institute of Massachusetts General Hospital (MGH), Massachusetts Institute of Technology (MIT), and Harvard, Cambridge, MA, United States., Hykes BL Jr; Department of Pathology and Immunology, Washington University School of Medicine, St. Louis, MO, United States., Mafunda NA; Ragon Institute of Massachusetts General Hospital (MGH), Massachusetts Institute of Technology (MIT), and Harvard, Cambridge, MA, United States., Hayward MR; Ragon Institute of Massachusetts General Hospital (MGH), Massachusetts Institute of Technology (MIT), and Harvard, Cambridge, MA, United States.; Department of Medicine, Harvard Medical School, Boston, MA, United States., Dong M; Ragon Institute of Massachusetts General Hospital (MGH), Massachusetts Institute of Technology (MIT), and Harvard, Cambridge, MA, United States.; Females Rising Through Education, Support, and Health (FRESH), Durban, South Africa., Dong KL; Ragon Institute of Massachusetts General Hospital (MGH), Massachusetts Institute of Technology (MIT), and Harvard, Cambridge, MA, United States.; Department of Medicine, Harvard Medical School, Boston, MA, United States.; Females Rising Through Education, Support, and Health (FRESH), Durban, South Africa., Gumbi T; Females Rising Through Education, Support, and Health (FRESH), Durban, South Africa.; Health Systems Trust, Durban, South Africa., Ceasar FX; Females Rising Through Education, Support, and Health (FRESH), Durban, South Africa.; Health Systems Trust, Durban, South Africa., Ismail N; HIV Pathogenesis Programme (HPP), The Doris Duke Medical Research Institute, University of KwaZulu-Natal, Durban, South Africa., Ndung'u T; Ragon Institute of Massachusetts General Hospital (MGH), Massachusetts Institute of Technology (MIT), and Harvard, Cambridge, MA, United States.; HIV Pathogenesis Programme (HPP), The Doris Duke Medical Research Institute, University of KwaZulu-Natal, Durban, South Africa.; Africa Health Research Institute (AHRI), Durban, South Africa.; Max Planck Institute for Infection Biology, Berlin, Germany.; Division of Infection and Immunity, University College London, London, United Kingdom., Gosmann C; Ragon Institute of Massachusetts General Hospital (MGH), Massachusetts Institute of Technology (MIT), and Harvard, Cambridge, MA, United States.; Department of Medicine, Harvard Medical School, Boston, MA, United States., Ghebremichael MS; Ragon Institute of Massachusetts General Hospital (MGH), Massachusetts Institute of Technology (MIT), and Harvard, Cambridge, MA, United States.; Department of Medicine, Harvard Medical School, Boston, MA, United States., Handley SA; Department of Pathology and Immunology, Washington University School of Medicine, St. Louis, MO, United States., Mitchell CM; Ragon Institute of Massachusetts General Hospital (MGH), Massachusetts Institute of Technology (MIT), and Harvard, Cambridge, MA, United States.; Department of Medicine, Harvard Medical School, Boston, MA, United States.; Department of Obstetrics and Gynecology, Massachusetts General Hospital, Boston, MA, United States., Villani AC; Department of Medicine, Harvard Medical School, Boston, MA, United States.; Center for Immunology and Inflammatory Diseases, Department of Medicine, Massachusetts General Hospital, Boston, MA, United States.; Broad Institute of MIT and Harvard, Immunology Program, Cambridge, MA, United States., Kwon DS; Ragon Institute of Massachusetts General Hospital (MGH), Massachusetts Institute of Technology (MIT), and Harvard, Cambridge, MA, United States.; Department of Medicine, Harvard Medical School, Boston, MA, United States.; Division of Infectious Diseases, Massachusetts General Hospital, Boston, MA, United States.

Publikováno v: Frontiers in cellular and infection microbiology [Front Cell Infect Microbiol] 2021 Sep 16; Vol. 11, pp. 733619. Date of Electronic Publication: 2021 Sep 16 (Print Publication: 2021).

The cervicovaginal mucus barrier to HIV-1 is diminished in bacterial vaginosis.

Autor: Hoang T; The Center for Nanomedicine, The Wilmer Eye Institute, Johns Hopkins University School of Medicine, Baltimore, Maryland, United States of America.; Department of Pharmacology and Molecular Sciences, Johns Hopkins University School of Medicine, Baltimore, Maryland, United States of America., Toler E; The Center for Nanomedicine, The Wilmer Eye Institute, Johns Hopkins University School of Medicine, Baltimore, Maryland, United States of America., DeLong K; The Center for Nanomedicine, The Wilmer Eye Institute, Johns Hopkins University School of Medicine, Baltimore, Maryland, United States of America.; Department of Ophthalmology, The Wilmer Eye Institute, Johns Hopkins University School of Medicine, Baltimore, Maryland, United States of America., Mafunda NA; Ragon Institute of MGH, MIT, and Harvard, Massachusetts General Hospital, Cambridge, Massachusetts, United States of America., Bloom SM; Ragon Institute of MGH, MIT, and Harvard, Massachusetts General Hospital, Cambridge, Massachusetts, United States of America.; Division of Infectious Diseases, Massachusetts General Hospital, Boston, Massachusetts, United States of America.; Harvard Medical School, Boston, Massachusetts, United States of America., Zierden HC; The Center for Nanomedicine, The Wilmer Eye Institute, Johns Hopkins University School of Medicine, Baltimore, Maryland, United States of America.; Department of Chemical and Biomolecular Engineering, Johns Hopkins University, Baltimore, Maryland, United States of America., Moench TR; Department of Biophysics, Johns Hopkins University, Baltimore, Maryland, United States of America., Coleman JS; Department of Gynecology and Obstetrics, Johns Hopkins University School of Medicine, Baltimore, Maryland, United States of America., Hanes J; The Center for Nanomedicine, The Wilmer Eye Institute, Johns Hopkins University School of Medicine, Baltimore, Maryland, United States of America.; Department of Pharmacology and Molecular Sciences, Johns Hopkins University School of Medicine, Baltimore, Maryland, United States of America.; Department of Ophthalmology, The Wilmer Eye Institute, Johns Hopkins University School of Medicine, Baltimore, Maryland, United States of America.; Department of Chemical and Biomolecular Engineering, Johns Hopkins University, Baltimore, Maryland, United States of America.; The Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins University, Baltimore, Maryland, United States of America., Kwon DS; Ragon Institute of MGH, MIT, and Harvard, Massachusetts General Hospital, Cambridge, Massachusetts, United States of America.; Division of Infectious Diseases, Massachusetts General Hospital, Boston, Massachusetts, United States of America.; Harvard Medical School, Boston, Massachusetts, United States of America., Lai SK; Division of Pharmacoengineering and Molecular Pharmaceutics, Eshelman School of Pharmacy, UNC/NCSU Joint Department of Biomedical Engineering, Department of Microbiology & Immunology, University of North Carolina-Chapel Hill, Chapel Hill, North Carolina, United States of America., Cone RA; Department of Biophysics, Johns Hopkins University, Baltimore, Maryland, United States of America., Ensign LM; The Center for Nanomedicine, The Wilmer Eye Institute, Johns Hopkins University School of Medicine, Baltimore, Maryland, United States of America.; Department of Pharmacology and Molecular Sciences, Johns Hopkins University School of Medicine, Baltimore, Maryland, United States of America.; Department of Ophthalmology, The Wilmer Eye Institute, Johns Hopkins University School of Medicine, Baltimore, Maryland, United States of America.; Department of Chemical and Biomolecular Engineering, Johns Hopkins University, Baltimore, Maryland, United States of America.; Department of Gynecology and Obstetrics, Johns Hopkins University School of Medicine, Baltimore, Maryland, United States of America.; The Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins University, Baltimore, Maryland, United States of America.

Publikováno v: PLoS pathogens [PLoS Pathog] 2020 Jan 23; Vol. 16 (1), pp. e1008236. Date of Electronic Publication: 2020 Jan 23 (Print Publication: 2020).