Zobrazeno 1 - 10
of 27
pro vyhledávání: '"Mafalda Escobar-Henriques"'
Autor:
Ira Buntenbroich, Vincent Anton, Daniel Perez-Hernandez, Tânia Simões, Felix Gaedke, Astrid Schauss, Gunnar Dittmar, Jan Riemer, Mafalda Escobar-Henriques
Publikováno v:
iScience, Vol 26, Iss 7, Pp 107014- (2023)
Summary: Defects in mitochondrial fusion are at the base of many diseases. Mitofusins power membrane-remodeling events via self-interaction and GTP hydrolysis. However, how exactly mitofusins mediate fusion of the outer membrane is still unclear. Str
Externí odkaz:
https://doaj.org/article/2eefab8f7d994a5ba3d1157baf69c35f
Publikováno v:
Bio-Protocol, Vol 11, Iss 22 (2021)
In this protocol, we describe the analysis of protein stability over time, using synthesis shutoff. As an example, we express HA-tagged yeast mitofusin Fzo1 in Saccharomyces cerevisiae and inhibit translation via cycloheximide (CHX). Proteasomal inhi
Externí odkaz:
https://doaj.org/article/6873d1974bae4d829bbcc5dde57ecdad
Publikováno v:
Frontiers in Cell and Developmental Biology, Vol 8 (2020)
Mitochondria entail an incredible dynamism in their morphology, impacting death signaling and selective elimination of the damaged organelles. In turn, by recycling the superfluous or malfunctioning mitochondria, mostly prevalent during aging, mitoph
Externí odkaz:
https://doaj.org/article/7cb8a5888f0a44eabc749eddde99b0e8
Autor:
Nadav Shai, Eden Yifrach, Carlo W. T. van Roermund, Nir Cohen, Chen Bibi, Lodewijk IJlst, Laetitia Cavellini, Julie Meurisse, Ramona Schuster, Lior Zada, Muriel C. Mari, Fulvio M. Reggiori, Adam L. Hughes, Mafalda Escobar-Henriques, Mickael M. Cohen, Hans R. Waterham, Ronald J. A. Wanders, Maya Schuldiner, Einat Zalckvar
Publikováno v:
Nature Communications, Vol 9, Iss 1, Pp 1-13 (2018)
The internal organization of the cell has been enriched by the discovery that organelles establish membrane contact sites, however the entire repertoire of these contacts is still being explored. Here the authors systematically identify the landscape
Externí odkaz:
https://doaj.org/article/2dc37c819f6140ea903c97bd975857d0
Publikováno v:
Frontiers in Physiology, Vol 10 (2019)
Mitochondria are dynamic organelles engaged in quality control and aging processes. They constantly undergo fusion, fission, transport, and anchoring events, which empower mitochondria with a very interactive behavior. The membrane remodeling process
Externí odkaz:
https://doaj.org/article/bd7d68eb0a3a4add849493afe8aff8fa
Publikováno v:
Bio-Protocol, Vol 8, Iss 22 (2018)
In this protocol we describe the separation and visualization of ubiquitylated forms of the yeast mitofusin Fzo1 by Western blot. To this aim, we express HA-tagged Fzo1 in Saccharomyces cerevisiae, break the cells to extract a membrane-enriched fract
Externí odkaz:
https://doaj.org/article/e7e2ab053e374e2b855ea6d536c2144a
Publikováno v:
eLife, Vol 7 (2018)
Cdc48/p97, a ubiquitin-selective chaperone, orchestrates the function of E3 ligases and deubiquitylases (DUBs). Here, we identify a new function of Cdc48 in ubiquitin-dependent regulation of mitochondrial dynamics. The DUBs Ubp12 and Ubp2 exert oppos
Externí odkaz:
https://doaj.org/article/091815149a064e6c82ccd4df8c9f26c2
Publikováno v:
Current Issues in Molecular Biology
Volume 35
Issue 1
Pages 35-58
Volume 35
Issue 1
Pages 35-58
Eukaryotic cells are subdivided into membrane-bound compartments specialized in different cellular functions and requiring dedicated sets of proteins. Although cells developed compartment-specific mechanisms for protein quality control, chaperones an
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8ece43247be648af6ec71744a08381d5
https://doi.org/10.21775/cimb.035.035
https://doi.org/10.21775/cimb.035.035
Publikováno v:
Bio Protoc
In this protocol, we describe the analysis of protein stability over time, using synthesis shutoff. As an example, we express HA-tagged yeast mitofusin Fzo1 in Saccharomyces cerevisiae and inhibit translation via cycloheximide (CHX). Proteasomal inhi
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::10e70427d4d364684a318e0afdf3a0cb
https://europepmc.org/articles/PMC8635844/
https://europepmc.org/articles/PMC8635844/
Autor:
Fabian den Brave, Ramona Schuster, Vincent Anton, Thomas Hermanns, Selver Altin, Gunnar Dittmar, Mafalda Escobar-Henriques, David Komander, R. Juergen Dohmen, Tania Simoes, Manuela K. Hospenthal
Publikováno v:
Life Science Alliance
LIFE SCIENCE ALLIANCE
LIFE SCIENCE ALLIANCE
Mitochondrial fusion requires a conformational change in α4 of the GTPase domain, followed by AAA-ATPase regulation, thus revealing striking mechanistic similarities between small and large GTPases.
Mitochondria are essential organelles whose f
Mitochondria are essential organelles whose f
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::04b8b19e744fc56c1d78433ba8c5ac39
https://pubmed.ncbi.nlm.nih.gov/31857350
https://pubmed.ncbi.nlm.nih.gov/31857350