Identification of BLNK and BTK as mediators of rituximab‐induced programmed cell death by CRISPR screens in GCB‐subtype diffuse large B‐cell lymphoma

Autor: Emil Aagaard Thomsen, Anne Bruun Rovsing, Mads Valdemar Anderson, Hanne Due, Jinrong Huang, Yonglun Luo, Karen Dybkær, Jacob Giehm Mikkelsen

Publikováno v: Molecular Oncology, Vol 14, Iss 9, Pp 1978-1997 (2020)

Diffuse large B‐cell lymphoma (DLBCL) is characterized by extensive genetic heterogeneity, and this results in unpredictable responses to the current treatment, R‐CHOP, which consists of a cancer drug combination supplemented with the humanized C

Externí odkaz: https://doaj.org/article/db68e49e73e744989b9cfb52fe01b91f

PegIT - A web-based design tool for prime editing

Autor: Jacob Giehm Mikkelsen, Emil Aagaard Thomsen, Mads Valdemar Anderson, Jonas Holst Wolff, Jakob Haldrup

Publikováno v: Anderson, M V, Haldrup, J, Thomsen, E A, Wolff, J H & Mikkelsen, J G 2021, ' PegIT-A web-based design tool for prime editing ', Nucleic Acids Research, vol. 49, no. W1, pp. W505-W509 . https://doi.org/10.1093/nar/gkab427
Nucleic Acids Research

Prime editing (PE) is a novel CRISPR-derived genome editing technique facilitating precision editing without double-stranded DNA breaks. PE, mediated by a Cas9-reverse transcriptase fusion protein, is based on dual-functioning prime editing guide RNA

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7b5102200071d87a9f767e2caf58977b
https://pure.au.dk/portal/da/publications/pegit--a-webbased-design-tool-for-prime-editing(4d855d3d-f3cf-49d3-ab33-ed1d7b0c1444).html

Targeted, homology-driven gene insertion in stem cells by ZFN-loaded ‘all-in-one’ lentiviral vectors

Autor: Jacob Giehm Mikkelsen, Yonglun Luo, Martin R. Jakobsen, Mads Valdemar Anderson, Yan Zhou, Yujia Cai, Shengting Li, Niels Uldbjerg, Anders Laustsen, Chenglong Sun

Publikováno v: eLife, Vol 5 (2016)
Cai, Y, Laustsen, A, Zhou, Y, Sun, C, Anderson, M V, Li, S, Uldbjerg, N, Luo, Y, Jakobsen, M R & Giehm Mikkelsen, J 2016, ' Targeted, homology-driven gene insertion in stem cells by ZFN-loaded 'all-in-one' lentiviral vectors ', eLife, vol. 5, e12213 . https://doi.org/10.7554/eLife.12213
eLife
Cai, Y, Laustsen, A, Zhou, Y, Sun, C, Anderson, M V, Li, S, Uldbjerg, N, Luo, Y, Jakobsen, M R & Mikkelsen, J G 2016, ' Targeted, homology-driven gene insertion in stem cells by ZFN-loaded 'all-in-one' lentiviral vectors ', eLife, vol. 5 . https://doi.org/10.7554/eLife.12213

Biased integration remains a key challenge for gene therapy based on lentiviral vector technologies. Engineering of next-generation lentiviral vectors targeting safe genomic harbors for insertion is therefore of high relevance. In a previous paper (C

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3b605e1ff7fcdfd7e57ec97903970e5f
https://elifesciences.org/articles/12213

LYRA, a webserver for lymphocyte receptor structural modeling

Autor: Michael Schantz Klausen, Paolo Marcatili, Morten Nielsen, Mads Valdemar Anderson, Martin Closter Jespersen

Publikováno v: Klausen, M S, Anderson, M V, Jespersen, M C, Nielsen, M & Marcatili, P 2015, ' LYRA, a webserver for lymphocyte receptor structural modeling. ', Nucleic Acids Research, vol. 43, no. W1, pp. W349-W355 . https://doi.org/10.1093/nar/gkv535
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Consejo Nacional de Investigaciones Científicas y Técnicas
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Nucleic Acids Research

The accurate structural modeling of B- and T-cell receptors is fundamental to gain a detailed insight in the mechanisms underlying immunity and in developing new drugs and therapies. The LYRA (LYmphocyte Receptor Automated modeling) web server (http:

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::824c2a0ae84d5bbe437ed91438fedd52
https://orbit.dtu.dk/en/publications/02ab83a1-042b-4abc-8949-70b9d3780d9a