Delivery of endocytosed proteins to the cell–division plane requires change of pathway from recycling to secretion

Autor: Sandra Richter, Marika Kientz, Sabine Brumm, Mads Eggert Nielsen, Misoon Park, Richard Gavidia, Cornelia Krause, Ute Voss, Hauke Beckmann, Ulrike Mayer, York-Dieter Stierhof, Gerd Jürgens

Publikováno v: eLife, Vol 3 (2014)

Membrane trafficking is essential to fundamental processes in eukaryotic life, including cell growth and division. In plant cytokinesis, post-Golgi trafficking mediates a massive flow of vesicles that form the partitioning membrane but its regulation

Externí odkaz: https://doaj.org/article/9924ec06328f4784aa590ae2d9c29e9f

ARF1 dimerization is essential for vesicle trafficking and dependent on activation by ARF-GEF dimers in Arabidopsis

Autor: York-Dieter Stierhof, Sandra Richter, Venkatesan Sundaresan, Gerd Juergens, Mads Eggert Nielsen, Sabine Brumm, Hauke Beckmann, Angela-Melanie Fischer, Manoj K. Singh

Membrane traffic maintains the organization of the eukaryotic cell and delivers cargo proteins to their subcellular destinations such as sites of action or degradation. Membrane vesicle formation requires ARF GTPase activation by the SEC7 domain of A

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::88b03dc27983a0b56bfb392eb8d95246
https://doi.org/10.1101/2020.01.21.913905

The plant membrane surrounding powdery mildew haustoria shares properties with the endoplasmic reticulum membrane

Autor: Henrik Böhlenius, Hans Thordal-Christensen, Mads Eggert Nielsen, Mark Kwaaitaal

Publikováno v: Journal of Experimental Botany
Kwaaitaal, M A C J, Nielsen, M E, Böhlenius, H & Thordal-Christensen, H 2017, ' The plant membrane surrounding powdery mildew haustoria shares properties with the endoplasmic reticulum membrane ', Journal of Experimental Botany, vol. 68, no. 21-22, pp. 5731-5743 . https://doi.org/10.1093/jxb/erx403

The plant-derived membrane surrounding the powdery mildew intracellular feeding structure, called the extrahaustorial membrane, accumulates the ER- and ER/cis-Golgi-associated Sar1 and Rab1 GTPases and stains with dyes for the ER.
Many filamento

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::74937fe5286742a0b543b1530195505c
https://pubmed.ncbi.nlm.nih.gov/29237056

A Split-GFP Gateway Cloning System for Topology Analyses of Membrane Proteins in Plants

Autor: Carsten Pedersen, Hans Thordal-Christensen, Mads Eggert Nielsen, Wenjun Xie

Publikováno v: PLoS ONE, Vol 12, Iss 1, p e0170118 (2017)
PLoS ONE
Xie, W, Nielsen, M E, Pedersen, C & Thordal-Christensen, H 2017, ' A split-GFP gateway cloning system for topology analyses of membrane proteins in plants ', P L o S One, vol. 12, no. 1, e0170118 . https://doi.org/10.1371/journal.pone.0170118

To understand the function of membrane proteins, it is imperative to know their topology. For such studies, a split green fluorescent protein (GFP) method is useful. GFP is barrel-shaped, consisting of 11 β-sheets. When the first ten β-sheets (GFP1

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2a310e001287ef041ea08bb4f5cd67e9
http://europepmc.org/articles/PMC5234810?pdf=render

The Multivesicular Body-Localized GTPase ARFA1b/1c Is Important for Callose Deposition and ROR2 Syntaxin-Dependent Preinvasive Basal Defense in Barley

Autor: Henrik Böhlenius, Dale Godfrey, Hans Thordal-Christensen, Mads Eggert Nielsen, Sara M. Mørch

Publikováno v: The Plant Cell. 22:3831-3844

Host cell vesicle traffic is essential for the interplay between plants and microbes. ADP-ribosylation factor (ARF) GTPases are required for vesicle budding, and we studied the role of these enzymes to identify important vesicle transport pathways in

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::3b5e56b7917a4567ebde79a9ae609a37
https://doi.org/10.1105/tpc.110.078063