Zobrazeno 1 - 10
of 15
pro vyhledávání: '"Madeleine Huber"'
Autor:
Madeleine Huber, Nico Vogel, Andreas Borst, Friedhelm Pfeiffer, Svetlana Karamycheva, Yuri I. Wolf, Eugene V. Koonin, Jörg Soppa
Publikováno v:
Frontiers in Microbiology, Vol 14 (2023)
Genomes of bacteria and archaea contain a much larger fraction of unidirectional (serial) gene pairs than convergent or divergent gene pairs. Many of the unidirectional gene pairs have short overlaps of −4 nt and −1 nt. As shown previously, trans
Externí odkaz:
https://doaj.org/article/2913daaa04154072820da5ef8a4ae4e5
Autor:
Madeleine Huber, Guilhem Faure, Sebastian Laass, Esther Kolbe, Kristina Seitz, Christina Wehrheim, Yuri I. Wolf, Eugene V. Koonin, Jörg Soppa
Publikováno v:
Nature Communications, Vol 10, Iss 1, Pp 1-11 (2019)
Archaea and bacteria often have gene pairs with overlapping stop and start codons, suggesting translational coupling. Here, Huber et al. analyse overlapping gene pairs from 720 genomes, and validate translational coupling via termination-reinitiation
Externí odkaz:
https://doaj.org/article/9290d1146bc24167a152ff0e2f436b2c
Autor:
Madeleine Huber, Jörg Soppa
The whole workflow for the quantification of specific transcripts by Northern blot analysis is described in detail, including RNA isolation, probe generation via labelling with Dig-dUTP, hybridization, signal visualization and quantification.
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::07ccfcf7d70097fef6e843077636b5e3
https://doi.org/10.21203/rs.2.11264/v1
https://doi.org/10.21203/rs.2.11264/v1
Autor:
Madeleine Huber, Jörg Soppa
The beta-Glucuronidase (GusA) is a long-known reporter enzyme for many different species [1]. The E. coli gusA gene is often used in plant research because plants lack an endogenous gusA gene. In E. coli, the transcript of the gusA gene is more stabl
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::4cc5883011003da711e1aa7511a1b7c9
https://doi.org/10.21203/rs.2.10596/v1
https://doi.org/10.21203/rs.2.10596/v1
Autor:
Jörg Soppa, Madeleine Huber
Glycerol-3-phosphate dehydrogenase (GlpD) is a recently introduced reporter enzyme for E. coli [1]. GlpD calalyzes the oxidation of Glycerin-3-phosphate (G3P) to dihydroxyacetone-phosphate (DHAP). The oxidation is coupled to the reduction of the arti
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::a42b6d443849714e801cf8f80dda324a
https://doi.org/10.21203/rs.2.10595/v1
https://doi.org/10.21203/rs.2.10595/v1
Autor:
Madeleine Huber, Jörg Soppa
The determination of translational efficiencies for single transcripts of interest is described for E. coli and H. volcanii. In both cases, the transcript levels are quantified by Northern blot analyses, and the protein levels are quantified by repor
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::1d18dbb6a376174e0cfc93db8c0f772e
https://doi.org/10.21203/rs.2.11363/v1
https://doi.org/10.21203/rs.2.11363/v1
Autor:
Madeleine Huber, Jörg Soppa
The dihydrofolate reductase (DHFR) is routinely used a reporter enzyme for H. volcanii. The DHFR catalyzes the reduction of dihydrofolate to tetrahydrofolate and the concomitant oxidation of NADPH to NADP+. This leads to a reduction of extinction at
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::f2703ea99581b9a33e4dedff0f30d846
https://doi.org/10.21203/rs.2.11263/v1
https://doi.org/10.21203/rs.2.11263/v1
Publikováno v:
ZERBITZUAN. :33-44
espanolEn las ultimas decadas, las politicas de inclusion social han venido marcadas por una perspectiva muy centrada en las personas en situacion de vulnerabilidad o de exclusion. Se trataba de individualizar las intervenciones con base en la idea d
Publikováno v:
Journal of Microbiological Methods. 131:181-187
Reporter genes facilitate the characterization of promoter activities, transcript stabilities, translational efficiencies, or intracellular localization. Various reporter genes for Escherichia coli have been established, however, most of them have dr
Autor:
Christian M. Lange, Karolin Zerulla, Petra Ludwig, Matthias Lehr, Madeleine Huber, Marius Wegener, Joerg Soppa
Publikováno v:
Microbiology (Reading, England). 164(4)
Translation initiation in 50–70 % of transcripts in Escherichia coli requires base pairing between the Shine–Dalgarno (SD) motif in the mRNA and the anti-SD motif at the 3′ end of the 16S rRNA. However, 30–50 % of E. coli transcripts are non-