Identification of the main barriers to Ku accumulation in chromatin

Autor: Madeleine Bossaert, Andrew T. Moreno, Antonio Peixoto, Marie-Jeanne Pillaire, Pauline Chanut, Philippe Frit, Patrick Calsou, Joseph J. Loparo, Sébastien Britton

Publikováno v: Cell Reports, Vol 43, Iss 8, Pp 114538- (2024)

Summary: Repair of DNA double-strand breaks by the non-homologous end-joining pathway is initiated by the binding of Ku to DNA ends. Multiple Ku proteins load onto linear DNAs in vitro. However, in cells, Ku loading is limited to ∼1–2 molecules p

Externí odkaz: https://doaj.org/article/a6f4ae965ac4434cb1366a88f8f04bc3

Dual targeting of higher-order DNA structures by azacryptands induces DNA junction-mediated DNA damage in cancer cells

Autor: Leïla Chouh, Anton Granzhan, Joanna Zell, David Monchaud, Nicolas Chéron, Katerina Duskova, Sébastien Britton, Madeleine Bossaert

Publikováno v: Nucleic Acids Research
Nucleic Acids Research, Oxford University Press, 2021, 49 (18), pp.10275-10288. ⟨10.1093/nar/gkab796⟩
Nucleic Acids Research, 2021, 49 (18), pp.10275-10288. ⟨10.1093/nar/gkab796⟩
Nucleic Acids Research, Oxford University Press, 2021, ⟨10.1093/nar/gkab796⟩

DNA is intrinsically dynamic and folds transiently into alternative higher-order structures such as G-quadruplexes (G4s) and three-way DNA junctions (TWJs). G4s and TWJs can be stabilised by small molecules (ligands) that have high chemotherapeutic p

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::94ea51047f31dc184646b87b1196a4bd
https://hal.archives-ouvertes.fr/hal-03372655

Transcription-associated topoisomerase 2α (TOP2A) activity is a major effector of cytotoxicity induced by G-quadruplex ligands

Autor: Olivier Bouchez, Patrick Calsou, Madeleine Bossaert, Linh-Trang Nguyên, Sébastien Britton, Jean-François Riou, Dennis Gomez, Angélique Pipier, Céline Noirot, Eric Defrancq, Remy-Felix Serre

Publikováno v: eLife
eLife, eLife Sciences Publication, 2021, 10, ⟨10.7554/eLife.65184⟩
eLife, Vol 10 (2021)

International audience; G-quadruplexes (G4) are non-canonical DNA structures found in the genome of most species including human. Small molecules stabilizing these structures, called G4 ligands, have been identified and, for some of them, shown to in

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::02c415173e60a18ef0d4106f45dd781a
http://europepmc.org/articles/PMC8279764

PAXX binding to the NHEJ machinery explains functional redundancy with XLF

Autor: Murielle Seif-El-Dahan, Antonia Kefala-Stavridi, Philippe Frit, Steven W. Hardwick, Dima Y. Chirgadze, Taiana Maia De Oliviera, Sébastien Britton, Nadia Barboule, Madeleine Bossaert, Arun Prasad Pandurangan, Katheryn Meek, Tom L. Blundell, Virginie Ropars, Patrick Calsou, Jean-Baptiste Charbonnier, Amanda K. Chaplin

Publikováno v: Science Advances. 9(22)

Nonhomologous end joining is a critical mechanism that repairs DNA double-strand breaks in human cells. In this work, we address the structural and functional role of the accessory protein PAXX [paralog of x-ray repair cross-complementing protein 4 (

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::76c59c31538f1bf322bca018f60f4a0f