A real time PCR platform for the simultaneous quantification of total and extrachromosomal HIV DNA forms in blood of HIV-1 infected patients.

Autor: Anna Casabianca, Chiara Orlandi, Benedetta Canovari, Maddalena Scotti, Marcello Acetoso, Massimo Valentini, Enzo Petrelli, Mauro Magnani

Publikováno v: PLoS ONE, Vol 9, Iss 11, p e111919 (2014)

The quantitative measurement of various HIV-1 DNA forms including total, unintegrated and integrated provirus play an increasingly important role in HIV-1 infection monitoring and treatment-related research. We report the development and validation o

Externí odkaz: https://doaj.org/article/346abe3b05da42f4aa208542322ea998

Differentiation of Promonocytic U937 Cells to Monocytes Is Associated with Reduced Mitochondrial Transport of Ascorbic Acid

Autor: Mara Fiorani, Orazio Cantoni, Andrea Guidarelli, Maddalena Scotti

Publikováno v: Oxidative Medicine and Cellular Longevity, Vol 2018 (2018)
Oxidative Medicine and Cellular Longevity

Growth of promonocytic U937 cells in the presence of DMSO promotes their differentiation to monocytes. After 4 days of culture in differentiating medium, these cells ceased to proliferate, displayed downregulated ryanodine receptor expression, and re

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fbbe09f50d58166ef8f02533444079d2
https://doaj.org/article/3ece41aaa5cb4e049857d5cc661876d8

Arsenite induces DNA damage via mitochondrial ROS and induction of mitochondrial permeability transition

Autor: Andrea, Guidarelli, Mara, Fiorani, Liana, Cerioni, Maddalena, Scotti, Orazio, Cantoni

Publikováno v: BioFactors (Oxford, England). 43(5)

Arsenite is an established DNA-damaging agent and human carcinogen. We initially selected conditions in which the metalloid causes DNA strand scission in the absence of detectable apoptotic DNA degradation in U937 cells. This response was suppressed

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::9ee6898228715921c3eee5463cc84c45
https://pubmed.ncbi.nlm.nih.gov/28703385

Gene expression profiles of epithelial cells microscopically isolated from a breast-invasive ductal carcinoma and a nodal metastasis

Autor: E. Mento, Alberto Albertini, V. Valsecchi, Barbara Simionati, S. Pilotti, Maddalena Scotti, Vladimir A. Kuznetsov, Rolland Reinbold, Renato Dulbecco, Paolo Vezzoni, Ileana Zucchi, E. Vicinanza, Giorgio Valle

Publikováno v: Proceedings of the National Academy of Sciences of the United States of America (Online) (2004).
info:cnr-pdr/source/autori:I Zucchi 1, E Mento, V A Kuznetsov, M Scotti, V Valsecchi, B Simionati, E Vicinanza, G Valle, S Pilotti, R Reinbold, P Vezzoni, A Albertini, R Dulbecco/titolo:Gene expression profiles of epithelial cells microscopically isolated from a breast-invasive ductal carcinoma and a nodal metastasis/doi:/rivista:Proceedings of the National Academy of Sciences of the United States of America (Online)/anno:2004/pagina_da:/pagina_a:/intervallo_pagine:/volume
Proceedings of the National Academy of Sciences of the United States of America 101 (2004): 18147–18152. doi:10.1073/pnas.0408260101
info:cnr-pdr/source/autori:Zucchi I, Mento E, Kuznetsov VA, Scotti M, Valsecchi V, Simionati B, Vicinanza E, Valle G, Pilotti S, Reinbold R, Vezzoni P, Albertini A, Dulbecco R./titolo:Gene expression profiles of epithelial cells microscopically isolated from a breast-invasive ductal carcinoma and a nodal metastasis/doi:10.1073%2Fpnas.0408260101/rivista:Proceedings of the National Academy of Sciences of the United States of America/anno:2004/pagina_da:18147/pagina_a:18152/intervallo_pagine:18147–18152/volume:101

Expression profiles of breast carcinomas are difficult to interpret when they are obtained from tissue in toto , which may contain a large proportion of non-cancer cells. To avoid this problem, we microscopically isolated cells from a primary invasiv

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5daf0387909fddc3e9ab9c697861089e
https://doi.org/10.1073/pnas.0408260101

U937 cell apoptosis induced by arsenite is prevented by low concentrations of mitochondrial ascorbic acid with hardly any effect mediated by the cytosolic fraction of the vitamin

Autor: Andrea, Guidarelli, Mara, Fiorani, Catia, Azzolini, Liana, Cerioni, Maddalena, Scotti, Orazio, Cantoni

Publikováno v: BioFactors (Oxford, England). 41(2)

Arsenite directly triggers cytochrome c and Smac/DIABLO release in mitochondria isolated from U937 cells. These effects were not observed in mitochondria pre-exposed for 15 min to 10 µM L-ascorbic acid (AA). In other experiments, intact cells treate

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::f91ceac4f73c0dbfe4df030cc718d577
https://pubmed.ncbi.nlm.nih.gov/25809564