Veni, Vidi, Vici: Immobilized Peptide-Based Conjugates as Tools for Capture, Analysis, and Transformation

Autor: Marta Kowalska, Dominik Popiel, Martyna Walter, Remigiusz Bąchor, Monika Biernat, Marek Cebrat, Monika Kijewska, Mariola Kuczer, Maciej Modzel, Alicja Kluczyk

Publikováno v: Chemosensors, Vol 10, Iss 1, p 31 (2022)

Analysis of peptide biomarkers of pathological states of the organism is often a serious challenge, due to a very complex composition of the cell and insufficient sensitivity of the current analytical methods (including mass spectrometry). One of the

Externí odkaz: https://doaj.org/article/8dc9d852095443a8b3c599c7214ed6f3

Ultraviolet Photodissociation of Protonated Peptides and Proteins Can Proceed with H/D Scrambling

Autor: Martin R. Larsen, Daniel T. Weltz Wollenberg, Morten Beck Trelle, Thomas J. D. Jørgensen, Maciej Modzel

Publikováno v: Modzel, M, Wollenberg, D T W, Trelle, M B, Larsen, M R & Jørgensen, T J D 2021, ' Ultraviolet Photodissociation of Protonated Peptides and Proteins Can Proceed with H/D Scrambling ', Analytical chemistry, vol. 93, no. 2, pp. 691–696 . https://doi.org/10.1021/acs.analchem.0c02957

Ultraviolet photodissociation (UVPD) has recently been introduced as an ion activation method for the determination of single-residue deuterium levels in H/D exchange tandem mass spectrometry experiments. In this regard, it is crucial to know which f

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::62d54d73703c7776bd089d38bc5c82de
https://doi.org/10.1021/acs.analchem.0c02957

Niemann-Pick C2 protein regulates sterol transport between plasma membrane and late endosomes in human fibroblasts

Autor: Christer S. Ejsing, Alice Dupont, Zane Berzina, Ahmed S Mehadi, Frederik W. Lund, Maria Szomek, Maria Louise V. Jensen, Maciej Modzel, Christian W. Heegaard, Lukasz M. Solanko, Katarzyna A. Solanko, Gitte Nielsen, Daniel Wüstner

Publikováno v: Berzina, Z, Solanko, L M, Mehadi, A S, Jensen, M L V, Lund, F W, Modzel, M, Szomek, M, Solanko, K A, Dupont, A, Nielsen, G K, Heegaard, C W, Ejsing, C S & Wüstner, D 2018, ' Niemann-Pick C2 protein regulates sterol transport between plasma membrane and late endosomes in human fibroblasts ', Chemistry and Physics of Lipids, vol. 213, pp. 48-61 . https://doi.org/10.1016/j.chemphyslip.2018.03.006

Niemann-Pick disease type C2 is a lipid storage disorder in which mutations in the NPC2 protein cause accumulation of lipoprotein-derived cholesterol in late endosomes and lysosomes (LE/LYSs). Whether cholesterol delivered by other means to NPC2 defi

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::03c02ec4c11006c8cf925d96428d0c0f
https://doi.org/10.1016/j.chemphyslip.2018.03.006

Quantification of prospective type 2 diabetes mellitus biomarkers by stable isotope dilution with bi-labeled standard glycated peptides

Autor: Piotr Stefanowicz, Tatiana Grischina, Anna Didio, Alena Soboleva, Andrej Frolov, Monika Kijewska, Tatiana Karonova, Halina Płóciennik, Vasily Stefanov, Maciej Modzel, Tatiana Bilova

Publikováno v: Analytical Methods. 9:409-418

Type 2 diabetes mellitus (T2DM) is a complex group of disorders, characterized by hyperglycemia, insulin resistance and insulin deficiency. In human blood, hyperglycemia ultimately results in the enhancement of glycation – a posttranslational modif

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::489b018ad67181e7a23eb5ad6456eb3f
https://doi.org/10.1039/c6ay02483a

Synthesis and Live-Cell Imaging of Fluorescent Sterols for Analysis of Intracellular Cholesterol Transport

Autor: Maciej, Modzel, Frederik W, Lund, Daniel, Wüstner

Publikováno v: Methods in molecular biology (Clifton, N.J.). 1583

Cellular cholesterol homeostasis relies on precise control of the sterol content of organelle membranes. Obtaining insight into cholesterol trafficking pathways and kinetics by live-cell imaging relies on two conditions. First, one needs to develop s

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::04a64c7d053ee28b2f30c93de77bf63d
https://pubmed.ncbi.nlm.nih.gov/28205171