Rapid and sensitive quantitation of glucose and glucose phosphates derived from storage carbohydrates using gas chromatography mass spectrometry

Autor: Ramon C. Sun, Macedo Jka, Corey O. Brizzee, Lyndsay E.A. Young, Matthew S. Gentry

Glycogen is the primary storage carbohydrate in mammals and it is synthesized in most tissues. Glycogen contains covalently attached phosphate groups on hydroxyls of glucose units. The addition of phosphate modulates branching pattern, granular size,

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4de4ee0353b0169d646a6b9136270f0e

Brain glycogen serves as a critical glucosamine cache required for protein glycosylation.

Autor: Sun RC; Department of Neuroscience, University of Kentucky, Lexington, KY, USA; Markey Cancer Center, University of Kentucky, Lexington, KY, USA. Electronic address: ramon.sun@uky.edu., Young LEA; Department of Molecular and Cellular Biochemistry, University of Kentucky, Lexington, KY, USA; Markey Cancer Center, University of Kentucky, Lexington, KY, USA., Bruntz RC; Department of Molecular and Cellular Biochemistry, University of Kentucky, Lexington, KY, USA., Markussen KH; Department of Molecular and Cellular Biochemistry, University of Kentucky, Lexington, KY, USA., Zhou Z; Department of Molecular and Cellular Biochemistry, University of Kentucky, Lexington, KY, USA., Conroy LR; Department of Neuroscience, University of Kentucky, Lexington, KY, USA; Markey Cancer Center, University of Kentucky, Lexington, KY, USA., Hawkinson TR; Department of Neuroscience, University of Kentucky, Lexington, KY, USA., Clarke HA; Department of Neuroscience, University of Kentucky, Lexington, KY, USA., Stanback AE; Department of Neuroscience, University of Kentucky, Lexington, KY, USA., Macedo JKA; Department of Molecular and Cellular Biochemistry, University of Kentucky, Lexington, KY, USA., Emanuelle S; Department of Molecular and Cellular Biochemistry, University of Kentucky, Lexington, KY, USA., Brewer MK; Department of Molecular and Cellular Biochemistry, University of Kentucky, Lexington, KY, USA; Institute for Research in Biomedicine, Barcelona, Spain., Rondon AL; Department of Molecular and Cellular Biochemistry, University of Kentucky, Lexington, KY, USA., Mestas A; Department of Molecular and Cellular Biochemistry, University of Kentucky, Lexington, KY, USA., Sanders WC; Department of Molecular and Cellular Biochemistry, University of Kentucky, Lexington, KY, USA., Mahalingan KK; Biochemistry and Molecular Biology, Indiana University School of Medicine, Indianapolis, IN, USA., Tang B; Biochemistry and Molecular Biology, Indiana University School of Medicine, Indianapolis, IN, USA., Chikwana VM; Biochemistry and Molecular Biology, Indiana University School of Medicine, Indianapolis, IN, USA., Segvich DM; Biochemistry and Molecular Biology, Indiana University School of Medicine, Indianapolis, IN, USA., Contreras CJ; Biochemistry and Molecular Biology, Indiana University School of Medicine, Indianapolis, IN, USA., Allenger EJ; Department of Physiology, University of Kentucky, Lexington, KY, USA., Brainson CF; Department of Toxicology and Cancer Biology, University of Kentucky, Lexington, KY, USA., Johnson LA; Department of Physiology, University of Kentucky, Lexington, KY, USA., Taylor RE; Department of Chemistry & Biochemistry and the Warren Center for Drug Discovery, University of Notre Dame, Notre Dame, IN, USA., Armstrong DD; Parasail LLC, Boston, MA, USA., Shaffer R; Enable Therapeutics, Boston, MA, USA., Waechter CJ; Department of Molecular and Cellular Biochemistry, University of Kentucky, Lexington, KY, USA., Vander Kooi CW; Department of Molecular and Cellular Biochemistry, University of Kentucky, Lexington, KY, USA; Markey Cancer Center, University of Kentucky, Lexington, KY, USA., DePaoli-Roach AA; Biochemistry and Molecular Biology, Indiana University School of Medicine, Indianapolis, IN, USA., Roach PJ; Biochemistry and Molecular Biology, Indiana University School of Medicine, Indianapolis, IN, USA., Hurley TD; Biochemistry and Molecular Biology, Indiana University School of Medicine, Indianapolis, IN, USA., Drake RR; Cell and Molecular Pharmacology and Experimental Therapeutics, Medical University of South Carolina, Charleston, SC, USA., Gentry MS; Department of Molecular and Cellular Biochemistry, University of Kentucky, Lexington, KY, USA; Markey Cancer Center, University of Kentucky, Lexington, KY, USA. Electronic address: matthew.gentry@uky.edu.

Publikováno v: Cell metabolism [Cell Metab] 2021 Jul 06; Vol. 33 (7), pp. 1404-1417.e9. Date of Electronic Publication: 2021 May 26.

Enhancing lifespan of budding yeast by pharmacological lowering of amino acid pools.

Autor: Hepowit NL; Department of Cell and Developmental Biology, Vanderbilt University, Nashville, TN 37240, USA., Macedo JKA; Department of Molecular and Cellular Biochemistry, University of Kentucky, Lexington, KY 40536, USA.; Markey Cancer Center, University of Kentucky, Lexington, KY 40536, USA., Young LEA; Department of Molecular and Cellular Biochemistry, University of Kentucky, Lexington, KY 40536, USA., Liu K; Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, Sichuan University, Chengdu 610000, Sichuan, P. R. China., Sun RC; Markey Cancer Center, University of Kentucky, Lexington, KY 40536, USA.; Department of Neuroscience, University of Kentucky, Lexington, KY 40536, USA., MacGurn JA; Department of Cell and Developmental Biology, Vanderbilt University, Nashville, TN 37240, USA., Dickson RC; Department of Molecular and Cellular Biochemistry, University of Kentucky, Lexington, KY 40536, USA.

Publikováno v: Aging [Aging (Albany NY)] 2021 Mar 21; Vol. 13 (6), pp. 7846-7871. Date of Electronic Publication: 2021 Mar 21.

Inhibition of Anaplerotic Glutaminolysis Underlies Selenite Toxicity in Human Lung Cancer.

Autor: Bruntz RC; Center for Environmental and Systems Biochemistry, Markey Cancer Center, and Department of Toxicology and Cancer Biology, University of Kentucky, Lexington, KY, 40536-0596, USA., Belshoff AC; Department of Chemistry, University of Louisville, Louisville, KY, 40292, USA., Zhang Y; Center for Environmental and Systems Biochemistry, Markey Cancer Center, and Department of Toxicology and Cancer Biology, University of Kentucky, Lexington, KY, 40536-0596, USA., Macedo JKA; Center for Environmental and Systems Biochemistry, Markey Cancer Center, and Department of Toxicology and Cancer Biology, University of Kentucky, Lexington, KY, 40536-0596, USA., Higashi RM; Center for Environmental and Systems Biochemistry, Markey Cancer Center, and Department of Toxicology and Cancer Biology, University of Kentucky, Lexington, KY, 40536-0596, USA., Lane AN; Center for Environmental and Systems Biochemistry, Markey Cancer Center, and Department of Toxicology and Cancer Biology, University of Kentucky, Lexington, KY, 40536-0596, USA., Fan TW; Center for Environmental and Systems Biochemistry, Markey Cancer Center, and Department of Toxicology and Cancer Biology, University of Kentucky, Lexington, KY, 40536-0596, USA.

Publikováno v: Proteomics [Proteomics] 2019 Nov; Vol. 19 (21-22), pp. e1800486. Date of Electronic Publication: 2019 Sep 12.