Výsledky vyhledávání - "Maaruthy Yelleswarapu"

A Multilayer Microfluidic Platform for the Conduction of Prolonged Cell-Free Gene Expression

Autor: Zoe Swank, Maaruthy Yelleswarapu, Tom F. A. de Greef, Sebastian J. Maerkl, Pascal A. Pieters, Ardjan J. van der Linden, Wilhelm T. S. Huck

Publikováno v: Journal of Visualized Experiments
Journal of Visualized Experiments (JoVE), 152:e59655. MYJoVE Corporation
Journal of Visualized Experiments, 152

The limitations of cell-based synthetic biology are becoming increasingly apparent as researchers aim to develop larger and more complex synthetic genetic regulatory circuits. The analysis of synthetic genetic regulatory networks in vivo is time cons

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8439e2c2e827579d65f2cd4ec7377b3b
https://doi.org/10.3791/59655-v

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Macromolecularly Crowded Protocells from Reversibly Shrinking Monodisperse Liposomes

Autor: Nan-Nan Deng, Mahesh A. Vibhute, Maaruthy Yelleswarapu, Hui Zhao, Lifei Zheng, Wilhelm T. S. Huck

Publikováno v: Journal of the American Chemical Society
Journal of the American Chemical Society, 140, 7399-7402
Journal of the American Chemical Society, 140, 24, pp. 7399-7402

The compartmentalization of cell-free gene expression systems in liposomes provides an attractive route to the formation of protocells, but these models do not capture the physical (crowded) environment found in living systems. Here, we present a mic

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c7a2f233d81dfa7a27b2b864913aa635
https://pubmed.ncbi.nlm.nih.gov/29870243

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Protein Synthesis in Coupled and Uncoupled Cell-Free Prokaryotic Gene Expression Systems

Autor: Aafke J. van Vugt-Jonker, Hans A. Heus, Roel J. M. Maas, Maaruthy Yelleswarapu, Wilhelm T. S. Huck, Maike M. K. Hansen, Marta Ventosa Rosquelles

Publikováno v: Acs Synthetic Biology, 5, 1433-1440
Acs Synthetic Biology, 5, 12, pp. 1433-1440

Secondary structure formation of mRNA, caused by desynchronization of transcription and translation, is known to impact gene expression in vivo. Yet, inactivation of mRNA by secondary structures in cell-free protein expression is frequently overlooke

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::690c5cfe5f8ab143740dd7a1ea6fbc38
https://pubmed.ncbi.nlm.nih.gov/27306580

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Monodisperse Uni- and Multicompartment Liposomes

Autor: Nan-Nan Deng, Wilhelm T. S. Huck, Maaruthy Yelleswarapu

Publikováno v: Journal of the American Chemical Society
Journal of the American Chemical Society, 138, 24, pp. 7584-7591
Journal of the American Chemical Society, 138, 7584-7591

Liposomes are self-assembled phospholipid vesicles with great potential in fields ranging from targeted drug delivery to artificial cells. The formation of liposomes using microfluidic techniques has seen considerable progress, but the liposomes form

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2fdeb9c3254bdcdeb5b2c4d32c6b1615

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Sigma Factor-Mediated Tuning of Bacterial Cell-Free Synthetic Genetic Oscillators

Autor: Emilien Dubuc, Wilhelm T. S. Huck, Ardjan J. van der Linden, Tom F. A. de Greef, Maaruthy Yelleswarapu, Pascal A. Pieters, Bob van Sluijs

Publikováno v: ACS Synthetic Biology
ACS Synthetic Biology, 7(12), 2879-2887. American Chemical Society

Cell-free transcription-translation provides a simplified prototyping environment to rapidly design and study synthetic networks. Despite the presence of a well characterized toolbox of genetic elements, examples of genetic networks that exhibit comp

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ffe0f7fc2c50ca44b98aa6c41c28d85e

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Microfluidic Assembly of Monodisperse Vesosomes as Artificial Cell Models

Autor: Nan-Nan Deng, Lifei Zheng, Wilhelm T. S. Huck, Maaruthy Yelleswarapu

Publikováno v: Journal of the American Chemical Society, 139, 587-590
Journal of the American Chemical Society, 139, 2, pp. 587-590
Journal of the American Chemical Society

Vesosomes are nested liposomal structures with high potential as advanced drug delivery vehicles, bioreactors and artificial cells. However, to date no method has been reported to prepare monodisperse vesosomes of controlled size. Here we report on a

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::50c00e10aa82acd69fa44010c5c821e6

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