Guide-free Cas9 from pathogenic Campylobacter jejuni bacteria causes severe damage to DNA

Autor: Deborah Horst-Kreft, Joyce H.G. Lebbink, Wiggert A. van Cappellen, Dior M. J. M. Beerens, Youri Hoogstrate, Johan W. Mouton, Gert-Jan Kremers, Rob Joosten, John van der Oost, Charlie Laffeber, Duncan J. H. Gaskin, Gaurav Dugar, Rogier Louwen, Jeroen Demmers, Serena T. Bruens, Andrew P. Stubbs, Chinmoy Saha, Maarten Klunder, Peter J. van der Spek, Dik C. van Gent, Prarthana Mohanraju, Peter van Baarlen

Publikováno v: Science Advances 6 (2020) 25
Science advances, 6(25):eaaz4849. American Association for the Advancement of Science
Science Advances, 6(25)

CRISPR-Cas9 systems are enriched in human pathogenic bacteria and have been linked to cytotoxicity by an unknown mechanism. Here, we show that upon infection of human cells, Campylobacter jejuni secretes its Cas9 (CjeCas9) nuclease into their cytopla

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5ac06800930fed35bc446b835458c41d
https://research.wur.nl/en/publications/guide-free-cas9-from-pathogenic-campylobacter-jejuni-bacteria-cau