Zobrazeno 1 - 10
of 36
pro vyhledávání: '"Maarten H. Geurts"'
Autor:
Martina Celotti, Lucca L.M. Derks, Johan van Es, Ruben van Boxtel, Hans Clevers, Maarten H. Geurts
Publikováno v:
STAR Protocols, Vol 5, Iss 3, Pp 103189- (2024)
Summary: Isogenic disease models, such as genetically engineered organoids, provide insight into the impact of genetic variants on organ function. Here, we present a protocol to create isogenic disease models from adult stem cell-derived organoids us
Externí odkaz:
https://doaj.org/article/4aa9ad949e6c44b6be56e5953d17244b
One-step generation of tumor models by base editor multiplexing in adult stem cell-derived organoids
Autor:
Maarten H. Geurts, Shashank Gandhi, Matteo G. Boretto, Ninouk Akkerman, Lucca L. M. Derks, Gijs van Son, Martina Celotti, Sarina Harshuk-Shabso, Flavia Peci, Harry Begthel, Delilah Hendriks, Paul Schürmann, Amanda Andersson-Rolf, Susana M. Chuva de Sousa Lopes, Johan H. van Es, Ruben van Boxtel, Hans Clevers
Publikováno v:
Nature Communications, Vol 14, Iss 1, Pp 1-18 (2023)
Abstract Optimization of CRISPR/Cas9-mediated genome engineering has resulted in base editors that hold promise for mutation repair and disease modeling. Here, we demonstrate the application of base editors for the generation of complex tumor models
Externí odkaz:
https://doaj.org/article/118466422c7b4a548268957a251cfb11
Autor:
Joep Beumer, Maarten H. Geurts, Mart M. Lamers, Jens Puschhof, Jingshu Zhang, Jelte van der Vaart, Anna Z. Mykytyn, Tim I. Breugem, Samra Riesebosch, Debby Schipper, Petra B. van den Doel, Wim de Lau, Cayetano Pleguezuelos-Manzano, Georg Busslinger, Bart L. Haagmans, Hans Clevers
Publikováno v:
Nature Communications, Vol 12, Iss 1, Pp 1-12 (2021)
Rapid identification of host genes essential for virus replication may expedite the generation of therapeutic interventions. Here the authors generate mutant clonal intestinal organoids for 19 host genes previously implicated in coronavirus biology a
Externí odkaz:
https://doaj.org/article/1db487b66712463886d067b96ac47dba
Autor:
Adriana Martinez-Silgado, Fjodor A. Yousef Yengej, Jens Puschhof, Veerle Geurts, Charelle Boot, Maarten H. Geurts, Maarten B. Rookmaaker, Marianne C. Verhaar, Joep Beumer, Hans Clevers
Publikováno v:
STAR Protocols, Vol 3, Iss 3, Pp 101639- (2022)
Summary: Intestinal organoids are three-dimensional cultures that resemble key aspects of the epithelium of origin. Here, we describe how to differentiate human small intestinal organoids by combining growth media variations and genetic engineering.
Externí odkaz:
https://doaj.org/article/dcf1297473074b90b8864176f730bcd6
Autor:
Maarten H. Geurts, Hans Clevers
Publikováno v:
Nature Reviews Bioengineering. 1:32-45
Publikováno v:
Journal of Visualized Experiments.
Autor:
Delilah Hendriks, Jos F. Brouwers, Karien Hamer, Maarten H. Geurts, Léa Luciana, Simone Massalini, Carmen López-Iglesias, Peter J. Peters, Maria J. Rodríguez-Colman, Susana Chuva de Sousa Lopes, Benedetta Artegiani, Hans Clevers
Publikováno v:
Nature Biotechnology. Nature Publishing Group
The lack of registered drugs for nonalcoholic fatty liver disease (NAFLD) is partly due to the paucity of human-relevant models for target discovery and compound screening. Here we use human fetal hepatocyte organoids to model the first stage of NAFL
Autor:
Victor Tirolle, Adrien Krug, Emma Bokobza, Mattijs Bulcaen, Marjolein M. Ensinck, Maarten H. Geurts, Delilah Hendriks, François Vermeulen, Frédéric Larbret, Alejandra Gutierrez-Guerrero, Louise Medaer, Rik Gijsbers, Philippe E. Mangeot, Hans Clevers, Marianne S. Carlon, Frederic Bost, Els Verhoeyen
Genome engineering has become more accessible thanks to the RNA programmable endonucleases such as the CRISPR/Cas9 system. However, using this editing technology in synthetic organs called ‘organoids’ is still very inefficient. This is due to the
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::bd862d8b5bbe9ad027009690e7538a3b
https://doi.org/10.1101/2022.08.04.502859
https://doi.org/10.1101/2022.08.04.502859
Autor:
Rosemary Millen, Willem W.B. De Kort, Mandy Koomen, Gijs J.F. van Son, Roán Gobits, Bas Penning de Vries, Harry Begthel, Maurice Zandvliet, Patricia Doornaert, Cornelis P.J. Raaijmakers, Maarten H. Geurts, Sjoerd G. Elias, Robert J.J. van Es, Remco de Bree, Lot A. Devriese, Stefan M. Willems, Onno Kranenburg, Else Driehuis, Hans Clevers
Publikováno v:
Med, 4(5), 290-310.e12
Background: Organoids are in vitro three-dimensional structures that can be grown from patient tissue. Head and neck cancer (HNC) is a collective term used for multiple tumor types including squamous cell carcinomas and salivary gland adenocarcinomas
Autor:
Johanna F. Dekkers, Maria Alieva, Astrid Cleven, Farid Keramati, Amber K. L. Wezenaar, Esmée J. van Vliet, Jens Puschhof, Peter Brazda, Inez Johanna, Angelo D. Meringa, Heggert G. Rebel, Maj-Britt Buchholz, Mario Barrera Román, Amber L. Zeeman, Sam de Blank, Domenico Fasci, Maarten H. Geurts, Annelisa M. Cornel, Else Driehuis, Rosemary Millen, Trudy Straetemans, Mara J. T. Nicolasen, Tineke Aarts-Riemens, Hendrikus C. R. Ariese, Hannah R. Johnson, Ravian L. van Ineveld, Froso Karaiskaki, Oded Kopper, Yotam E. Bar-Ephraim, Kai Kretzschmar, Alexander M. M. Eggermont, Stefan Nierkens, Ellen J. Wehrens, Henk G. Stunnenberg, Hans Clevers, Jürgen Kuball, Zsolt Sebestyen, Anne C. Rios
Publikováno v:
Nature Biotechnology. Nature Publishing Group
Extending the success of cellular immunotherapies against blood cancers to the realm of solid tumors will require improved in vitro models that reveal therapeutic modes of action at the molecular level. Here we describe a system, called BEHAV3D, deve