Zobrazeno 1 - 10
of 43
pro vyhledávání: '"MS2-MCP"'
Publikováno v:
eLife, Vol 12 (2023)
It is well known that enhancers regulate the spatiotemporal expression of their target genes by recruiting transcription factors (TFs) to the cognate binding sites in the region. However, the role of multiple binding sites for the same TFs and their
Externí odkaz:
https://doaj.org/article/f871881b1f6844159f679c7137604c72
Autor:
Bohong Chen, Shengcheng Deng, Tianyu Ge, Miaoman Ye, Jianping Yu, Song Lin, Wenbin Ma, Zhou Songyang
Publikováno v:
Protein & Cell, Vol 11, Iss 9, Pp 641-660 (2020)
Abstract In mammalian cells, long noncoding RNAs (lncRNAs) form complexes with proteins to execute various biological functions such as gene transcription, RNA processing and other signaling activities. However, methods to track endogenous lncRNA dyn
Externí odkaz:
https://doaj.org/article/d25fcbe6094f4ba6bfdf13c5e6ab422d
Autor:
Mark Tingey, Steven J. Schnell, Wenlan Yu, Jason Saredy, Samuel Junod, Dhrumil Patel, Abdullah A. Alkurdi, Weidong Yang
Publikováno v:
Cells, Vol 11, Iss 19, p 3079 (2022)
The transient nature of RNA has rendered it one of the more difficult biological targets for imaging. This difficulty stems both from the physical properties of RNA as well as the temporal constraints associated therewith. These concerns are further
Externí odkaz:
https://doaj.org/article/4c5d090503524a7ba2f22229197a1587
Publikováno v:
Animal Models and Experimental Medicine, Vol 2, Iss 3, Pp 185-190 (2019)
Abstract Background Catalytic defect Cas9‐cytosine deaminase fusion is widely used in base editing. The Multiple copy numbers of the MS2 binding site (MBS) can recruit multiple MS2 coat proteins (MCPs), which are usually applied to amplify signals.
Externí odkaz:
https://doaj.org/article/912f3c2b7eaf43d29221e6668d2b9f13
The transient nature of RNA has rendered it one of the more difficult biological targets for imaging. This difficulty stems both from the physical properties of RNA as well as the temporal constraints associated therewith. These concerns are further
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::706a477ba37345aec3ab4853f2a0d60a
Akademický článek
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Publikováno v:
Animal Models and Experimental Medicine
Animal Models and Experimental Medicine, Vol 2, Iss 3, Pp 185-190 (2019)
Animal Models and Experimental Medicine, Vol 2, Iss 3, Pp 185-190 (2019)
Background Catalytic defect Cas9‐cytosine deaminase fusion is widely used in base editing. The Multiple copy numbers of the MS2 binding site (MBS) can recruit multiple MS2 coat proteins (MCPs), which are usually applied to amplify signals. Our stud
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::42b280524bfddf9a266435dece584fb7
http://europepmc.org/articles/PMC6762045
http://europepmc.org/articles/PMC6762045
Publikováno v:
Methods in Molecular Biology
Methods in Molecular Biology, pp.121-144, 2020, ⟨10.1007/978-1-0716-0712-1_7⟩
Pichon, X, Robert, M C, Bertrand, E, Singer, R H & Tutucci, E 2020, New Generations of MS2 Variants and MCP Fusions to Detect Single mRNAs in Living Eukaryotic Cells . in M Heinlein (ed.), RNA Tagging : Methods and Protocols . Methods in molecular biology (MIMB), vol. 2166, Humana, New York, NY, pp. 121-144 . https://doi.org/10.1007/978-1-0716-0712-1_7
Methods Mol Biol
Methods in Molecular Biology ISBN: 9781071607114
RNA Tagging: Methods and Protocols, 121-144
STARTPAGE=121;ENDPAGE=144;TITLE=RNA Tagging
Methods in Molecular Biology, pp.121-144, 2020, ⟨10.1007/978-1-0716-0712-1_7⟩
Pichon, X, Robert, M C, Bertrand, E, Singer, R H & Tutucci, E 2020, New Generations of MS2 Variants and MCP Fusions to Detect Single mRNAs in Living Eukaryotic Cells . in M Heinlein (ed.), RNA Tagging : Methods and Protocols . Methods in molecular biology (MIMB), vol. 2166, Humana, New York, NY, pp. 121-144 . https://doi.org/10.1007/978-1-0716-0712-1_7
Methods Mol Biol
Methods in Molecular Biology ISBN: 9781071607114
RNA Tagging: Methods and Protocols, 121-144
STARTPAGE=121;ENDPAGE=144;TITLE=RNA Tagging
Live imaging of single RNA from birth to death brought important advances in our understanding of the spatiotemporal regulation of gene expression. These studies have provided a comprehensive understanding of RNA metabolism by describing the process
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e85f071ce5ed337cc197411337c6a73f
https://hal.archives-ouvertes.fr/hal-03044742/document
https://hal.archives-ouvertes.fr/hal-03044742/document
Akademický článek
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Autor:
Robert H. Singer, Evelina Tutucci, Roy Parker, Jennifer F. Garcia, Maria Vera, Jeetayu Biswas
Publikováno v:
Nature methods
The MS2-MCP system enables researchers to image multiple steps of the mRNA life cycle with high temporal and spatial resolution. However, for short-lived mRNAs, the tight binding of the MS2 coat protein (MCP) to the MS2 binding sites (MBS) protects t
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4fcbdfc1bb468c0599b7989f7510eabb
http://europepmc.org/articles/PMC5843578
http://europepmc.org/articles/PMC5843578